Infection and re-infection of
Leptospira spp
cat
dog
microagglutination test
phylogenetic analysis
polymerase chain reaction
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
May 2024
May 2024
Historique:
received:
18
12
2023
accepted:
11
04
2024
medline:
24
6
2024
pubmed:
24
6
2024
entrez:
24
6
2024
Statut:
ppublish
Résumé
Leptospirosis is a re-emerging zoonosis that is under-reported in tropical countries, and canines can be a potential reservoir of the disease. The objective of this study was to diagnose A sample of 200 animals, including dogs and cats from the animal protection programs of Bogota, Colombia, were used in this study. Blood was collected from these animals for serum and DNA analysis. Conventional polymerase chain reaction (PCR) was performed using the 16s rRNA primer set, and higher-quality amplification products were sequenced by Sanger. For serodiagnosis, a group of PCR-positive samples was tested using the microagglutination test (MAT). The overall PCR positivity of stray dogs and cats was 56%, 52.9%, and 65.3% in dogs and cats, respectively. The MAT seropositivity was 77.3%, and only dogs showed titers higher than 1:400. These results showed that stray dogs and cats were previously exposed to different serovars of
Sections du résumé
Background and Aim
UNASSIGNED
Leptospirosis is a re-emerging zoonosis that is under-reported in tropical countries, and canines can be a potential reservoir of the disease. The objective of this study was to diagnose
Materials and Methods
UNASSIGNED
A sample of 200 animals, including dogs and cats from the animal protection programs of Bogota, Colombia, were used in this study. Blood was collected from these animals for serum and DNA analysis. Conventional polymerase chain reaction (PCR) was performed using the 16s rRNA primer set, and higher-quality amplification products were sequenced by Sanger. For serodiagnosis, a group of PCR-positive samples was tested using the microagglutination test (MAT).
Results
UNASSIGNED
The overall PCR positivity of stray dogs and cats was 56%, 52.9%, and 65.3% in dogs and cats, respectively. The MAT seropositivity was 77.3%, and only dogs showed titers higher than 1:400.
Conclusion
UNASSIGNED
These results showed that stray dogs and cats were previously exposed to different serovars of
Identifiants
pubmed: 38911095
doi: 10.14202/vetworld.2024.973-980
pii: Vetworld-17-973
pmc: PMC11188880
doi:
Types de publication
Journal Article
Langues
eng
Pagination
973-980Informations de copyright
Copyright: © Puentes, et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.