Cytotoxic effects of the cigarette smoke extract of heated tobacco products on human oral squamous cell carcinoma: the role of reactive oxygen species and CaMKK2.
Humans
Reactive Oxygen Species
/ metabolism
Mouth Neoplasms
/ metabolism
Cell Line, Tumor
Smoke
/ adverse effects
Carcinoma, Squamous Cell
/ metabolism
Calcium-Calmodulin-Dependent Protein Kinase Kinase
/ metabolism
Tobacco Products
/ adverse effects
Apoptosis
/ drug effects
Nicotiana
/ chemistry
Calcium
/ metabolism
Cell Survival
/ drug effects
Calcium
Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)
Heated tobacco products (HTPs)
Oral cancer
Reactive oxygen species (ROS)
Journal
The journal of physiological sciences : JPS
ISSN: 1880-6562
Titre abrégé: J Physiol Sci
Pays: Japan
ID NLM: 101262417
Informations de publication
Date de publication:
25 Jun 2024
25 Jun 2024
Historique:
received:
26
03
2024
accepted:
08
06
2024
medline:
26
6
2024
pubmed:
26
6
2024
entrez:
25
6
2024
Statut:
epublish
Résumé
The increasing prevalence of heated tobacco products (HTPs) has heightened concerns regarding their potential health risks. Previous studies have demonstrated the toxicity of cigarette smoke extract (CSE) from traditional tobacco's mainstream smoke, even after the removal of nicotine and tar. Our study aimed to investigate the cytotoxicity of CSE derived from HTPs and traditional tobacco, with a particular focus on the role of reactive oxygen species (ROS) and intracellular Ca A human oral squamous cell carcinoma (OSCC) cell line, HSC-3 was utilized. To prepare CSE, aerosols from HTPs (IQOS) and traditional tobacco products (1R6F reference cigarette) were collected into cell culture media. A cell viability assay, apoptosis assay, western blotting, and Fluo-4 assay were conducted. Changes in ROS levels were measured using electron spin resonance spectroscopy and the high-sensitivity 2',7'-dichlorofluorescein diacetate assay. We performed a knockdown of calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) by shRNA lentivirus in OSCC cells. CSE from both HTPs and traditional tobacco exhibited cytotoxic effects in OSCC cells. Exposure to CSE from both sources led to an increase in intracellular Ca Our findings suggest that the CSE from both HTPs and traditional tobacco induce cytotoxicity. This toxicity is mediated by ROS, which are regulated through Ca
Sections du résumé
BACKGROUND
BACKGROUND
The increasing prevalence of heated tobacco products (HTPs) has heightened concerns regarding their potential health risks. Previous studies have demonstrated the toxicity of cigarette smoke extract (CSE) from traditional tobacco's mainstream smoke, even after the removal of nicotine and tar. Our study aimed to investigate the cytotoxicity of CSE derived from HTPs and traditional tobacco, with a particular focus on the role of reactive oxygen species (ROS) and intracellular Ca
METHODS
METHODS
A human oral squamous cell carcinoma (OSCC) cell line, HSC-3 was utilized. To prepare CSE, aerosols from HTPs (IQOS) and traditional tobacco products (1R6F reference cigarette) were collected into cell culture media. A cell viability assay, apoptosis assay, western blotting, and Fluo-4 assay were conducted. Changes in ROS levels were measured using electron spin resonance spectroscopy and the high-sensitivity 2',7'-dichlorofluorescein diacetate assay. We performed a knockdown of calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) by shRNA lentivirus in OSCC cells.
RESULTS
RESULTS
CSE from both HTPs and traditional tobacco exhibited cytotoxic effects in OSCC cells. Exposure to CSE from both sources led to an increase in intracellular Ca
CONCLUSION
CONCLUSIONS
Our findings suggest that the CSE from both HTPs and traditional tobacco induce cytotoxicity. This toxicity is mediated by ROS, which are regulated through Ca
Identifiants
pubmed: 38918702
doi: 10.1186/s12576-024-00928-1
pii: 10.1186/s12576-024-00928-1
doi:
Substances chimiques
Reactive Oxygen Species
0
Smoke
0
Calcium-Calmodulin-Dependent Protein Kinase Kinase
EC 2.7.11.17
CAMKK2 protein, human
EC 2.7.11.17
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35Informations de copyright
© 2024. The Author(s).
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