Doping control analysis of myo-inositol trispyrophosphate and 10 bisphosphonates in equine plasma by ion chromatography-mass spectrometry and its application to clodronic acid horse administration.
IC‐HRMS
bisphosphonates
clodronic acid
doping control
myo‐inositol trispyrophosphate (ITPP)
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
25 Jun 2024
25 Jun 2024
Historique:
revised:
03
06
2024
received:
31
03
2024
accepted:
06
06
2024
medline:
26
6
2024
pubmed:
26
6
2024
entrez:
26
6
2024
Statut:
aheadofprint
Résumé
Bisphosphonates and myo-inositol trispyrophosphate (ITPP) are two classes of difficult-to-detect polar drugs that are prohibited under the rules of racing. ITPP is a drug capable of increasing the amount of oxygen in hypoxic tissues, and studies have shown that administration of ITPP increases the maximal exercise capacity in mice. The properties of ITPP make it an ideal candidate as a doping agent to enhance performance in racehorses. In recent years, ITPP had indeed been detected in racehorses and confiscated items. As for bisphosphonates, it is especially critical to control their use as since February 2019, the International Agreement on Breeding, Racing and Wagering (IABRW) by the International Federation of Horseracing Authorities (IFHA) had identified specific conditions on which bisphosphonates should not be administered to a racehorse. A recent review of literature shows that there is yet a simultaneous screening method for detecting ITPP and bisphosphonates in equine samples. This paper describes an efficient ion chromatography high-resolution mass spectrometry (IC-HRMS) method for the simultaneous detection of ITPP and 10 bisphosphonates at sub-parts-per-billion (ppb) to low-ppb levels in equine plasma after solid-phase extraction (SPE) and its application to an administration study of clodronic acid in horses.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024 John Wiley & Sons Ltd.
Références
Drake MT, Clarke BL, Khosla S. Bisphosphonates: mechanism of action and role in clinical practice. Mayo Clin Proc. 2008;83(9):1032‐1045. doi:10.4065/83.9.1032
International Federation of Horseracing Authorities (IFHA). Article 6 in the International Agreement on Breeding. Racing and Wagering (IABRW). https://www.ifhaonline.org/default.asp?section=IABRW&AREA=2#article6. Accessed Jun 28, 2023
Watanabe T, Takeda T, Omiya S, et al. Reduction in hemoglobin‐oxygen affinity results in the improvement of exercise capacity in mice with chronic heart failure. J am Coll Cardiol. 2008;52(9):779‐786. doi:10.1016/j.jacc.2008.06.003
Biolo A, Greferath R, Siwik DA, et al. Enhanced exercise capacity in mice with severe heart failure treated with an allosteric effector of hemoglobin, myo‐inositol trispyrophosphate. P Natl Acad Sci USA. 2009;106(6):1926‐1929. doi:10.1073/pnas.0812381106
Wong ASY, Ho ENM, Wan TSM. Detection of myo‐inositol trispyrophosphate in equine urine and plasma by hydrophilic interaction chromatography‐tandem mass spectrometry. Drug Test Anal. 2012;4(5):355‐361. doi:10.1002/dta.397
Wong ASY, Ho ENM, Wan TSM, Lam KKH, Stewart BD. Liquid chromatography‐mass spectrometry analysis of five bisphosphonates in equine urine and plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;998‐999:1‐7. doi:10.1016/j.jchromb.2015.06.020
Lam G, Zhao S, Sandhu J, Yi R, Loganathan D, Morrissey B. Detection of myo‐inositol tris pyrophosphate (ITPP) in equine following an administration of ITPP. Drug Test Anal. 2014;6(3):268‐276. doi:10.1002/dta.1473
Görgens C, Guddat S, Schänzer W, Thevis M. Screening and confirmation of myo‐inositol trispyrophosphate (ITPP) in human urine by hydrophilic interaction liquid chromatography high resolution/high accuracy mass spectrometry for doping control purposes. Drug Test Anal. 2014;6(11–12):1102‐1107. doi:10.1002/dta.1700
Görgens C, Guddat S, Orlovius AK, et al. “Dilute‐and‐inject” multi‐target screening assay for highly polar doping agents using hydrophilic interaction liquid chromatography high resolution/high accuracy mass spectrometry for sports drug testing. Anal Bioanal Chem. 2015;407(18):5365‐5379. doi:10.1007/s00216‐015‐8699‐x
Zacharis CK, Tzanavaras PD. Determination of bisphosphonate active pharmaceutical ingredients in pharmaceuticals and biological material: a review of analytical methods. J Pharm Biomed Anal. 2008;48(3):483‐496. doi:10.1016/j.jpba.2008.05.028
Manousi N, Tzanavaras PD, Zacharis CK. Determination of bisphosphonate active pharmaceutical ingredients in pharmaceuticals and biological materials: an updated review. J Pharm Biomed Anal. 2022;219:114921. doi:10.1016/j.jpba.2022.114921
Knych HK, McKemie DS, Yim S, Stanley SD, Arthur RM. Long‐term monitoring of clodronate in equine hair using liquid chromatography‐tandem mass spectrometry. J Chromatogr B. 2023;1226:123789. doi:10.1016/j.jchromb.2023.123789
Garcia P, Pinètre J, Morel S, et al. An innovative derivatization‐free IC‐MS/MS method for the detection of bisphosphonates in horse plasma. Drug Test Anal. 2020;12(10):1452‐1461. doi:10.1002/dta.2892
Knych HK, Janes J, Kennedy L, et al. Detection and residence time of bisphosphonates in bone of horses. J Vet Diagn Invest. 2022;34(1):23‐27. doi:10.1177/10406387211050049
Riggs CM, Thompson SL, So YM, et al. Tiludronic acid can be detected in blood and urine samples from Thoroughbred racehorses over 3 years after last administration. Equine Vet J. 2021;53(6):1287‐1295. doi:10.1111/evj.13395
AORC. AORC Guidelines for the Minimum Criteria for Identification by Chromatography and Mass Spectrometry. Accessed April 25, 2024. https://www.aorc-online.org/documents/aorc-ms-criteria-ilacg7/1aorc-ms-criteria-mar-24.pdf
Brooks L, Loganathan D, Cooper C, et al. Elimination profile of clodronate in plasma and urine after a single intramuscular (IM) administration of 720 ng clodronate (Osphos®) to Standardbred mares. In: Proceedings of the 22nd International Conference of Racing Analysts and Veterinarians, Dubai, Emirates. Regent Press Co. Ltd.; 2018:385‐393.
de Oliveira K, Fachiolli DF, Watanabe MJ, et al. Dimethylglycine supplementation in horses performing incremental treadmill exercise. Comp Exerc Physiol. 2015;11:167‐172.
Kalman DS, Feldman S, Scheinberg AR, Krieger DR, Bloomer RJ. Influence of methylsulfonylmethane on markers of exercise recovery and performance in healthy men: a pilot study. J Int Soc Sports Nutr. 2012;9(1):46. doi:10.1186/1550‐2783‐9‐46