The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production.
BacMam
Baculovirus
Cell culture
Insect cells
Mammalian cells
Protein expression
Protein purification
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
26
6
2024
Statut:
ppublish
Résumé
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production in various types of mammalian cell lines. We decided to establish BacMam in our lab in order to streamline our workflows for gene expression in insect and mammalian cells, as it is straightforward to parallelize the baculovirus generation for both types of eukaryotic cells. This chapter provides a step-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.
Identifiants
pubmed: 38926271
doi: 10.1007/978-1-0716-3878-1_3
doi:
Substances chimiques
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-53Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Smith GE, Summers MD, Fraser MJ (1983) Production of human beta interferon in insect cells infected with a Baculovirus expression vector. Mol Cell Biol 3:2156–2165. https://doi.org/10.1128/mcb.3.12.2156-2165.1983
doi: 10.1128/mcb.3.12.2156-2165.1983
pubmed: 6318086
pmcid: 370086
Stolt-Bergner P, Benda C, Bergbrede T, Besir H, Celie PHN, Chang C, Drechsel D, Fischer A, Geerlof A, Giabbai B, Van Den Heuvel J, Huber G, Knecht W, Lehner A, Lemaitre R, Nordén K, Pardee G, Racke I, Remans K, Sander A, Scholz J, Stadnik M, Storici P, Weinbruch D, Zaror I, Lua LHL, Suppmann S (2018) Baculovirus-driven protein expression in insect cells: a benchmarking study. J Struct Biol 203:71–80. https://doi.org/10.1016/j.jsb.2018.03.004
doi: 10.1016/j.jsb.2018.03.004
pubmed: 29545204
Sari D, Gupta K, Raj DBTG, Aubert A, Drncová P, Garzoni F, Fitzgerald D, Berger I (2016) The MultiBac Baculovirus/insect cell expression vector system for producing complex protein biologics. In: Vega MC (ed) Advanced technologies for protein complex production and characterization. Springer International Publishing, Cham, pp 199–215
doi: 10.1007/978-3-319-27216-0_13
Van Oers MM, Pijlman GP, Vlak JM (2015) Thirty years of baculovirus–insect cell protein expression: from dark horse to mainstream technology. J Gen Virol 96:6–23. https://doi.org/10.1099/vir.0.067108-0
doi: 10.1099/vir.0.067108-0
pubmed: 25246703
Chambers AC, Aksular M, Graves LP, Irons SL, Possee RD, King LA (2018) Overview of the baculovirus expression system. Curr Protoc Protein Sci 91:5.4.1-5.4.6. https://doi.org/10.1002/cpps.47
doi: 10.1002/cpps.47
pubmed: 29516481
Luckow VA, Lee SC, Barry GF, Olins PO (1993) Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol 67:4566–4579. https://doi.org/10.1128/jvi.67.8.4566-4579.1993
doi: 10.1128/jvi.67.8.4566-4579.1993
pubmed: 8392598
pmcid: 237841
Luckow VA (1993) Baculovirus systems for the expression of human gene products. Curr Opin Biotechnol 4:564–572. https://doi.org/10.1016/0958-1669(93)90078-B
doi: 10.1016/0958-1669(93)90078-B
pubmed: 7764207
Berger I, Fitzgerald DJ, Richmond TJ (2004) Baculovirus expression system for heterologous multiprotein complexes. Nat Biotechnol 22:1583–1587. https://doi.org/10.1038/nbt1036
doi: 10.1038/nbt1036
pubmed: 15568020
Possee RD, Hitchman RB, Richards KS, Mann SG, Siaterli E, Nixon CP, Irving H, Assenberg R, Alderton D, Owens RJ, King LA (2008) Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnol Bioeng 101:1115–1122. https://doi.org/10.1002/bit.22002
doi: 10.1002/bit.22002
pubmed: 18781697
Lemaitre RP, Bogdanova A, Borgonovo B, Woodruff JB, Drechsel DN (2019) FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing. BMC Biotechnol 19:20. https://doi.org/10.1186/s12896-019-0512-z
doi: 10.1186/s12896-019-0512-z
pubmed: 30925874
pmcid: 6441187
O’Reilly DR, Miller L, Luckow VA (1994) Baculovirus expression vectors: a laboratory manual. Oxford University Press, New York
Hofmann C, Sandig V, Jennings G, Rudolph M, Schlag P, Strauss M (1995) Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA 92:10099–10103. https://doi.org/10.1073/pnas.92.22.10099
doi: 10.1073/pnas.92.22.10099
pubmed: 7479733
pmcid: 40743
Boyce FM, Bucher NL (1996) Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA 93:2348–2352. https://doi.org/10.1073/pnas.93.6.2348
doi: 10.1073/pnas.93.6.2348
pubmed: 8637876
pmcid: 39799
Jäger V, Büssow K, Schirrmann T (2015) Transient recombinant protein expression in mammalian cells. In: Al-Rubeai M (ed) Animal Cell Culture. Springer International Publishing, Cham, pp 27–64
doi: 10.1007/978-3-319-10320-4_2
Almo SC, Love JD (2014) Better and faster: improvements and optimization for mammalian recombinant protein production. Curr Opin Struct Biol 26:39–43. https://doi.org/10.1016/j.sbi.2014.03.006
doi: 10.1016/j.sbi.2014.03.006
pubmed: 24721463
pmcid: 4766836
Hunter M, Yuan P, Vavilala D, Fox M (2019) Optimization of protein expression in mammalian cells. Curr Protoc Protein Sci 95:e77. https://doi.org/10.1002/cpps.77
doi: 10.1002/cpps.77
pubmed: 30265450
Kost TA, Condreay JP (2002) Recombinant baculoviruses as mammalian cell gene-delivery vectors. Trends Biotechnol 20:173–180. https://doi.org/10.1016/S0167-7799(01)01911-4
doi: 10.1016/S0167-7799(01)01911-4
pubmed: 11906750
Condreay JP, Witherspoon SM, Clay WC, Kost TA (1999) Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA 96:127–132. https://doi.org/10.1073/pnas.96.1.127
doi: 10.1073/pnas.96.1.127
pubmed: 9874783
pmcid: 15104
Miyamura T, Aizaki H, Tani H, Shoji I, Matsuura Y, Ishii K, Chiba T, Saito I (1997) Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol 78:2657–2664. https://doi.org/10.1099/0022-1317-78-10-2657
doi: 10.1099/0022-1317-78-10-2657
pubmed: 9349488
Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SCF, Taylor V, Ballmer-Hofer K, Berger I, Berger P (2016) Highly efficient baculovirus-mediated multigene delivery in primary cells. Nat Commun 7:11529. https://doi.org/10.1038/ncomms11529
doi: 10.1038/ncomms11529
pubmed: 27143231
pmcid: 4857464
Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC (2008) BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies. Protein Expr Purif 62:160–170. https://doi.org/10.1016/j.pep.2008.08.004
doi: 10.1016/j.pep.2008.08.004
pubmed: 18782620
pmcid: 2637115
Pearce KH, Overton LK, Gampe RT, Barrett GB, Taylor JD, McKee DD, Campobasso N, Nolte RT, Reid RA (2019) BacMam production and crystal structure of nonglycosylated apo human furin at 1.89 Å resolution. Acta Crystallogr F Struct Biol Commun 75:239–245. https://doi.org/10.1107/S2053230X19001419
doi: 10.1107/S2053230X19001419
pubmed: 30950824
pmcid: 6450522
Scott MJ, Modha SS, Rhodes AD, Broadway NM, Hardwicke PI, Zhao HJ, Kennedy-Wilson KM, Sweitzer SM, Martin SL (2007) Efficient expression of secreted proteases via recombinant BacMam virus. Protein Expr Purif 52:104–116. https://doi.org/10.1016/j.pep.2006.09.017
doi: 10.1016/j.pep.2006.09.017
pubmed: 17129735
Mahajan P, Ellis K, Mukhopadhyay S, Fernandez-Cid A, Chi G, Man H, Dürr KL, Burgess-Brown NA (2021) Expression screening of human integral membrane proteins using BacMam. In: Chen YW, Yiu C-PB (eds) Structural genomics. Springer US, New York, pp 95–115
doi: 10.1007/978-1-0716-0892-0_6
Puthenveetil R, Lee C, Banerjee A (2020) Production of recombinant transmembrane proteins from mammalian cells for biochemical and structural analyses. Curr Protoc Cell Biol 87:e106. https://doi.org/10.1002/cpcb.106
doi: 10.1002/cpcb.106
pubmed: 32515556
pmcid: 7373347
Guo W, Wang M, Chen L (2022) A co-expression vector for baculovirus-mediated protein expression in mammalian cells. Biochem Biophys Res Commun 594:69–73. https://doi.org/10.1016/j.bbrc.2022.01.056
doi: 10.1016/j.bbrc.2022.01.056
pubmed: 35074588
Miller-Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS (2020) Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. eLife 9:e62611. https://doi.org/10.7554/eLife.62611
doi: 10.7554/eLife.62611
pubmed: 33236988
pmcid: 7785296
Goehring A, Lee C-H, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E (2014) Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Nat Protoc 9:2574–2585. https://doi.org/10.1038/nprot.2014.173
doi: 10.1038/nprot.2014.173
pubmed: 25299155
pmcid: 4291175
Fornwald JA, Lu Q, Boyce FM, Ames RS (2016) Gene expression in mammalian cells using BacMam, a modified Baculovirus system. In: Murhammer DW (ed) Baculovirus and insect cell expression protocols. Springer, New York, pp 95–116
doi: 10.1007/978-1-4939-3043-2_5
Ames RS, Kost TA, Condreay JP (2007) BacMam technology and its application to drug discovery. Expert Opin Drug Discov 2:1669–1681. https://doi.org/10.1517/17460441.2.12.1669
doi: 10.1517/17460441.2.12.1669
pubmed: 23488908
Wright PD, Veale EL, McCoull D, Tickle DC, Large JM, Ococks E, Gothard G, Kettleborough C, Mathie A, Jerman J (2017) Terbinafine is a novel and selective activator of the two-pore domain potassium channel TASK3. Biochem Biophys Res Commun 493:444–450. https://doi.org/10.1016/j.bbrc.2017.09.002
doi: 10.1016/j.bbrc.2017.09.002
pubmed: 28882594
Takata Y, Kishine H, Sone T, Andoh T, Nozaki M, Poderycki M, Chesnut JD, Imamoto F (2011) Generation of iPS cells using a BacMam multigene expression system. Cell Struct Funct 36:209–222. https://doi.org/10.1247/csf.11008
doi: 10.1247/csf.11008
pubmed: 21979235
Hindriksen S, Bramer AJ, Truong MA, Vromans MJM, Post JB, Verlaan-Klink I, Snippert HJ, Lens SMA, Hadders MA (2017) Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells. PLoS One 12:e0179514. https://doi.org/10.1371/journal.pone.0179514
doi: 10.1371/journal.pone.0179514
pubmed: 28640891
pmcid: 5480884
Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren P-O, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I (2022) Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus. Nucleic Acids Res 50:7783–7799. https://doi.org/10.1093/nar/gkac587
doi: 10.1093/nar/gkac587
pubmed: 35801912
pmcid: 9303279
Puente-Massaguer E, Cajamarca-Berrezueta B, Volart A, González-Domínguez I, Gòdia F (2022) Transduction of HEK293 cells with BacMam Baculovirus is an efficient system for the production of HIV-1 virus-like particles. Viruses 14:636. https://doi.org/10.3390/v14030636
doi: 10.3390/v14030636
pubmed: 35337043
pmcid: 8954388
Thompson CM, Petiot E, Mullick A, Aucoin MG, Henry O, Kamen AA (2015) Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems. BMC Biotechnol 15:31. https://doi.org/10.1186/s12896-015-0152-x
doi: 10.1186/s12896-015-0152-x
pubmed: 25981500
pmcid: 4432999
Schaly S, Ghebretatios M, Prakash S (2021) Baculoviruses in gene therapy and personalized medicine. Biologics 15:115–132. https://doi.org/10.2147/BTT.S292692
doi: 10.2147/BTT.S292692
pubmed: 33953541
pmcid: 8088983
Graves L, Aksular M, Alakeely R, Ruiz Buck D, Chambers A, Murguia-Meca F, Plata-Muñoz J-J, Hughes S, Johnson P, Possee R, King L (2018) Improved Baculovirus vectors for transduction and gene expression in human pancreatic islet cells. Viruses 10:574. https://doi.org/10.3390/v10100574
doi: 10.3390/v10100574
pubmed: 30347797
pmcid: 6213606
Irons SL, Chambers AC, Lissina O, King LA, Possee RD (2018) Protein production using the Baculovirus expression system. Curr Protoc Protein Sci. https://doi.org/10.1002/cpps.45
Lawrence A-M, Besir H (2009) Staining of proteins in gels with Coomassie G-250 without organic solvent and acetic acid. JoVE:1350. https://doi.org/10.3791/1350
Berger I, Garzoni F, Chaillet M, Haffke M, Gupta K, Aubert A (2013) The MultiBac protein complex production platform at the EMBL. JoVE:50159. https://doi.org/10.3791/50159
Gorda B, Toelzer C, Aulicino F, Berger I (2021) The MultiBac BEVS: basics, applications, performance and recent developments. Methods Enzymol 660:129–154
doi: 10.1016/bs.mie.2021.06.018
pubmed: 34742385
Thermo Fischer Scientific (2018) Bac-to-Bac™ Baculovirus expression system USER GUIDE
Mahajan P, Strain-Damerell C, Mukhopadhyay S, Fernandez-Cid A, Gileadi O, Burgess-Brown NA (2021) Screening and production of recombinant human proteins: protein production in insect cells. In: Chen YW, Yiu C-PB (eds) Structural genomics. Springer US, New York, pp 67–94
doi: 10.1007/978-1-0716-0892-0_5