TGF-β Isoforms and Local Environments Greatly Modulate Biological Nature of Human Retinal Pigment Epithelium Cells.
3D culture
TGF-β isoforms
human retinal pigment epithelium
hypoxia
seahorse cellular metabolic functions
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
07 Jun 2024
07 Jun 2024
Historique:
received:
30
04
2024
revised:
02
06
2024
accepted:
05
06
2024
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
27
6
2024
Statut:
epublish
Résumé
To characterize transforming growth factor-β (TGF-β) isoform (TGF-β1~3)-b's biological effects on the human retinal pigment epithelium (RPE) under normoxia and hypoxia conditions, ARPE19 cells cultured by 2D (two-dimensional) and 3D (three-dimensional) conditions were subjected to various analyses, including (1) an analysis of barrier function by trans-epithelial electrical resistance (TEER) measurements; (2) qPCR analysis of major ECM molecules including
Identifiants
pubmed: 38927817
pii: bioengineering11060581
doi: 10.3390/bioengineering11060581
pii:
doi:
Types de publication
Journal Article
Langues
eng