Application of Stem Cells Shows Antiinflammatory Effect in an Irradiated Random Pattern Flap Model.
ASC
HUVEC
MSC
irradiation
random pattern flaps
Journal
Journal of personalized medicine
ISSN: 2075-4426
Titre abrégé: J Pers Med
Pays: Switzerland
ID NLM: 101602269
Informations de publication
Date de publication:
23 May 2024
23 May 2024
Historique:
received:
27
04
2024
revised:
17
05
2024
accepted:
21
05
2024
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
27
6
2024
Statut:
epublish
Résumé
In reconstructive surgery, local flaps might develop tissue necrosis or partial flap loss especially after previous irradiation, which may be necessary in many tumor entities. The application of stem cells seems promising to improve flap perfusion and might be a possible solution to optimize flap survival. Twenty rats received harvesting of bilateral random pattern fasciocutaneous flaps. The right flaps received 20 Gy ionizing radiation 4 weeks prior to the surgery, while the left flaps served as the non-irradiated control. After flap harvest, four different stem cell mixtures (5 × 10 Application of ASCs, ASCs-HUVECs and MSCs resulted in a lower number of CD68-stained cells compared to the no cell group. The expression of Hif1α was higher in the ASC group compared to those in the MSC and previously treated no cell groups. Treatment with MSCs and MSCs-HUVECs prevented shrinking of the flaps in this series. Application of ASCs, MSCs and ASCs-HUVECs was shown to have an antiinflammatory effect. Treatment with MSCs and MSCs-HUVECs can prevent early shrinking of the flaps.
Sections du résumé
BACKGROUND
BACKGROUND
In reconstructive surgery, local flaps might develop tissue necrosis or partial flap loss especially after previous irradiation, which may be necessary in many tumor entities. The application of stem cells seems promising to improve flap perfusion and might be a possible solution to optimize flap survival.
METHODS
METHODS
Twenty rats received harvesting of bilateral random pattern fasciocutaneous flaps. The right flaps received 20 Gy ionizing radiation 4 weeks prior to the surgery, while the left flaps served as the non-irradiated control. After flap harvest, four different stem cell mixtures (5 × 10
RESULTS
RESULTS
Application of ASCs, ASCs-HUVECs and MSCs resulted in a lower number of CD68-stained cells compared to the no cell group. The expression of Hif1α was higher in the ASC group compared to those in the MSC and previously treated no cell groups. Treatment with MSCs and MSCs-HUVECs prevented shrinking of the flaps in this series.
CONCLUSION
CONCLUSIONS
Application of ASCs, MSCs and ASCs-HUVECs was shown to have an antiinflammatory effect. Treatment with MSCs and MSCs-HUVECs can prevent early shrinking of the flaps.
Identifiants
pubmed: 38929774
pii: jpm14060554
doi: 10.3390/jpm14060554
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : ELAN Fund of the Medical Faculty of the Friedrich-Alexander University Erlangen-Nurnberg (FAU
ID : (ELAN P094 / 21-04-13-1-Müller-Seubert)
Organisme : Borhild Marshall fund as well as the Forschungsstiftung Medizin of the University Hospital Erlangen at Friedrich Alexander University Erlangen-Nuernberg FAU
ID : 34496666 HP-ZW_Forschungsstiftung Medizin