Characterization of neutralizing chimeric heavy-chain antibodies against tetanus toxin.
Tetanus toxin
heavy chain antibody
nanobody
neutralizing antibody
phage display library
Journal
Human vaccines & immunotherapeutics
ISSN: 2164-554X
Titre abrégé: Hum Vaccin Immunother
Pays: United States
ID NLM: 101572652
Informations de publication
Date de publication:
31 Dec 2024
31 Dec 2024
Historique:
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
27
6
2024
Statut:
ppublish
Résumé
Tetanus toxin (TeNT) is one of the most toxic proteins. Neutralizing antibodies against TeNT are effective in prevention and treatment. In this study, 14 anti-tetanus nanobodies were obtained from a phage display nanobody library by immunizing a camel with the C-terminal receptor-binding domain of TeNT (TeNT-Hc) as the antigen. After fusion with the human Fc fragment, 11 chimeric heavy-chain antibodies demonstrated nanomolar binding toward TeNT-Hc. The results of toxin neutralization experiments showed that T83-7, T83-8, and T83-13 completely protected mice against 20 × the median lethal dose (LD
Identifiants
pubmed: 38934499
doi: 10.1080/21645515.2024.2366641
doi:
Substances chimiques
Tetanus Toxin
0
Antibodies, Neutralizing
0
Single-Domain Antibodies
0
Immunoglobulin Heavy Chains
0
Antibodies, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM