Exploration of Trichoderma reesei as an alternative host for erythritol production.
Trichoderma reesei
Design of experiments
Erythritol
Polyols
Journal
Biotechnology for biofuels and bioproducts
ISSN: 2731-3654
Titre abrégé: Biotechnol Biofuels Bioprod
Pays: England
ID NLM: 9918300888906676
Informations de publication
Date de publication:
27 Jun 2024
27 Jun 2024
Historique:
received:
16
03
2024
accepted:
18
06
2024
medline:
28
6
2024
pubmed:
28
6
2024
entrez:
27
6
2024
Statut:
epublish
Résumé
Erythritol, a natural polyol, is a low-calorie sweetener synthesized by a number of microorganisms, such as Moniliella pollinis. Yet, a widespread use of erythritol is limited by high production costs due to the need for cultivation on glucose-rich substrates. This study explores the potential of using Trichoderma reesei as an alternative host for erythritol production, as this saprotrophic fungus can be cultivated on lignocellulosic biomass residues. The objective of this study was to evaluate whether such an alternative host would lead to a more sustainable and economically viable production of erythritol by identifying suitable carbon sources for erythritol biosynthesis, the main parameters influencing erythritol biosynthesis and evaluating the feasibility of scaling up the defined process. Our investigation revealed that T. reesei can synthesize erythritol from glucose but not from other carbon sources like xylose and lactose. T. reesei is able to consume erythritol, but it does not in the presence of glucose. Among nitrogen sources, urea and yeast extract were more effective than ammonium and nitrate. A significant impact on erythritol synthesis was observed with variations in pH and temperature. Despite successful shake flask experiments, the transition to bioreactors faced challenges, indicating a need for further scale-up optimization. While T. reesei shows potential for erythritol production, reaching a maximum concentration of 1 g/L over an extended period, its productivity could be improved by optimizing the parameters that affect erythritol production. In any case, this research contributes valuable insights into the polyol metabolism of T. reesei, offering potential implications for future research on glycerol or mannitol production. Moreover, it suggests a potential metabolic association between erythritol production and glycolysis over the pentose phosphate pathway.
Sections du résumé
BACKGROUND
BACKGROUND
Erythritol, a natural polyol, is a low-calorie sweetener synthesized by a number of microorganisms, such as Moniliella pollinis. Yet, a widespread use of erythritol is limited by high production costs due to the need for cultivation on glucose-rich substrates. This study explores the potential of using Trichoderma reesei as an alternative host for erythritol production, as this saprotrophic fungus can be cultivated on lignocellulosic biomass residues. The objective of this study was to evaluate whether such an alternative host would lead to a more sustainable and economically viable production of erythritol by identifying suitable carbon sources for erythritol biosynthesis, the main parameters influencing erythritol biosynthesis and evaluating the feasibility of scaling up the defined process.
RESULTS
RESULTS
Our investigation revealed that T. reesei can synthesize erythritol from glucose but not from other carbon sources like xylose and lactose. T. reesei is able to consume erythritol, but it does not in the presence of glucose. Among nitrogen sources, urea and yeast extract were more effective than ammonium and nitrate. A significant impact on erythritol synthesis was observed with variations in pH and temperature. Despite successful shake flask experiments, the transition to bioreactors faced challenges, indicating a need for further scale-up optimization.
CONCLUSIONS
CONCLUSIONS
While T. reesei shows potential for erythritol production, reaching a maximum concentration of 1 g/L over an extended period, its productivity could be improved by optimizing the parameters that affect erythritol production. In any case, this research contributes valuable insights into the polyol metabolism of T. reesei, offering potential implications for future research on glycerol or mannitol production. Moreover, it suggests a potential metabolic association between erythritol production and glycolysis over the pentose phosphate pathway.
Identifiants
pubmed: 38937852
doi: 10.1186/s13068-024-02537-x
pii: 10.1186/s13068-024-02537-x
doi:
Types de publication
Journal Article
Langues
eng
Pagination
90Subventions
Organisme : Christian Doppler Forschungsgesellschaft
ID : CD-lab CAZy
Informations de copyright
© 2024. The Author(s).
Références
Rice T, Zannini E, Arendt EK, Coffey A. A review of polyols - biotechnological production, food applications, regulation, labeling and health effects. Crit Rev Food Sci Nutr. 2020;60(12):2034–51. https://doi.org/10.1080/10408398.2019.1625859 .
doi: 10.1080/10408398.2019.1625859
pubmed: 31210053
Kunduru KR, Hogerat R, Ghosal K, Shaheen-Mualim M, Farah S. Renewable polyol-based biodegradable polyesters as greener plastics for industrial applications. Chem Eng J. 2023;459: 141211. https://doi.org/10.1016/j.cej.2022.141211 .
doi: 10.1016/j.cej.2022.141211
Uses - European Association of Polyol Producers. https://polyols-eu.org/uses/ . Accessed May 2 2024.
Martău GA, Coman V, Vodnar DC. Recent advances in the biotechnological production of erythritol and mannitol. Crit Rev Biotechnol. 2020;40(5):608–22. https://doi.org/10.1080/07388551.2020.1751057 .
doi: 10.1080/07388551.2020.1751057
pubmed: 32299245
Regnat K, Mach RL, Mach-Aigner AR. Erythritol as sweetener-wherefrom and whereto? Appl Microbiol Biotechnol. 2018;102(2):587–95. https://doi.org/10.1007/s00253-017-8654-1 .
doi: 10.1007/s00253-017-8654-1
pubmed: 29196787
Embuscado ME. Chapter 8: polyols. In: Spillane WJ, editor. Optimising sweet taste in foods. Sawston: Woodhead Publishing; 2006. p. 153–74. https://doi.org/10.1533/9781845691646.2.153 .
doi: 10.1533/9781845691646.2.153
Liang P, Cao M, Li J, Wang Q, Dai Z. Expanding sugar alcohol industry: microbial production of sugar alcohols and associated chemocatalytic derivatives. Biotechnol Adv. 2023;64: 108105. https://doi.org/10.1016/j.biotechadv.2023.108105 .
doi: 10.1016/j.biotechadv.2023.108105
pubmed: 36736865
Khatape AB, Dastager SG, Rangaswamy V. An overview of erythritol production by yeast strains. FEMS Microbiol Lett. 2022;369(1): fnac107. https://doi.org/10.1093/femsle/fnac107 .
doi: 10.1093/femsle/fnac107
pubmed: 36354105
Rzechonek DA, Dobrowolski A, Rymowicz W, Mirończuk AM. Recent advances in biological production of erythritol. Crit Rev Biotechnol. 2018;38(4):620–33. https://doi.org/10.1080/07388551.2017.1380598 .
doi: 10.1080/07388551.2017.1380598
pubmed: 28954540
Daza-Serna L, Serna-Loaiza S, Masi A, Mach RL, Mach-Aigner AR, Friedl A. From the culture broth to the erythritol crystals: an opportunity for circular economy. Appl Microbiol Biotechnol. 2021;105(11):4467–86. https://doi.org/10.1007/s00253-021-11355-2 .
doi: 10.1007/s00253-021-11355-2
pubmed: 34043080
pmcid: 8195806
Jeffries TW, Jin YS. Metabolic engineering for improved fermentation of pentoses by yeasts. Appl Microbiol Biotechnol. 2004;63(5):495–509. https://doi.org/10.1007/s00253-003-1450-0 .
doi: 10.1007/s00253-003-1450-0
pubmed: 14595523
Directive - 2009/41/EC. http://data.europa.eu/eli/dir/2009/41/oj . Accessed 4 May 2024.
EFSA Panel on Genetically Modified Organisms (GMO). Scientific Opinion on Guidance on the risk assessment of genetically modified microorganisms and their products intended for food and feed use. EFSA J. 2011;9(6):2193. https://doi.org/10.2903/j.efsa.2011.2193 .
doi: 10.2903/j.efsa.2011.2193
Vázquez-Vuelvas O, Cervantes-Chávez JA, Delgado-Virgen FJ, Valdez-Velázquez LL, Osuna-Cisneros RJ. Chapter 7: fungal bioprocessing of lignocellulosic materials for biorefinery. In: De Mandal S, Passari AK, editors. Recent advancement in microbial technology. Cambridge: Academic Press; 2021. p. 171–208.
doi: 10.1016/B978-0-12-822098-6.00009-4
Sánchez C. Lignocellulosic residues: biodegradation and bioconversion by fungi. Biotechnol Adv. 2009;27(2):185–94. https://doi.org/10.1016/j.biotechadv.2008.11.00 .
doi: 10.1016/j.biotechadv.2008.11.00
pubmed: 19100826
de Passos DF, Pereira N, de Castro AM. A comparative review of recent advances in cellulases production by Aspergillus, Penicillium and Trichoderma strains and their use for lignocellulose deconstruction. Curr Opin Green Sustain Chem. 2018;14:60–6.
doi: 10.1016/j.cogsc.2018.06.003
Wang MY, Hou J. Biorefinery of Lignocellulosics for Biofuels and Biochemicals. Quality Living through Chemurgy and Green Chemistry. 2016;143–91. https://doi.org/10.1016/j.cogsc.2018.06.003 .
Bischof RH, Ramoni J, Seiboth B. Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei. Microb Cell Fact. 2016;15(1):106. https://doi.org/10.1186/s12934-016-0507-6 .
doi: 10.1186/s12934-016-0507-6
pubmed: 27287427
pmcid: 4902900
Fischer AJ, Maiyuran S, Yaver DS. Industrial relevance of Trichoderma reesei as an enzyme producer. Methods Mol Biol. 2021;2234:23–43. https://doi.org/10.1007/978-1-0716-1048-0_223 .
doi: 10.1007/978-1-0716-1048-0_223
pubmed: 33165776
Meyer V, Basenko EY, Benz JP, Braus GH, Caddick MX, Csukai M, et al. Growing a circular economy with fungal biotechnology: a white paper. Fungal Biol Biotechnol. 2020;7:5. https://doi.org/10.1186/s40694-020-00095-z .
doi: 10.1186/s40694-020-00095-z
pubmed: 32280481
pmcid: 7140391
Jovanović B, Mach RL, Mach-Aigner AR. Erythritol production on wheat straw using Trichoderma reesei. AMB Express. 2014;4:34. https://doi.org/10.1186/s13568-014-0034-y .
doi: 10.1186/s13568-014-0034-y
pubmed: 24949268
pmcid: 4052684
Gryshyna A, Kautto L, Peterson R, Nevalainen H. On the safety of filamentous fungi with special emphasis on Trichoderma reesei and products made by recombinant means. In: Schmoll M, Dattenböck C, editors. Gene expression systems in fungi: advancements and applications fungal biology. Cham: Springer; 2016. https://doi.org/10.1007/978-3-319-27951-0_20 .
doi: 10.1007/978-3-319-27951-0_20
Frisvad JC, Møller LLH, Larsen TO, Kumar R, Arnau J. Safety of the fungal workhorses of industrial biotechnology: update on the mycotoxin and secondary metabolite potential of Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei. Appl Microbiol Biotechnol. 2018;102(22):9481–515. https://doi.org/10.1007/s00253-018-9354-1 .
doi: 10.1007/s00253-018-9354-1
pubmed: 30293194
pmcid: 6208954
Metz B, de Vries RP, Polak S, Seidl V, Seiboth B. The Hypocrea jecorina (syn. Trichoderma reesei) lxr1 gene encodes a D-mannitol dehydrogenase and is not involved in L-arabinose catabolism. FEBS Lett. 2009;583(8):1309–13. https://doi.org/10.1016/j.febslet.2009.03.027 .
doi: 10.1016/j.febslet.2009.03.027
pubmed: 19303876
Dashtban M, Kepka G, Seiboth B, Qin W, Dashtban M, Kepka G, et al. Xylitol production by genetically engineered Trichoderma reesei strains using barley straw as feedstock. Appl Biochem Biotechnol. 2013;169(2):554–69. https://doi.org/10.1007/s12010-012-0008-y .
doi: 10.1007/s12010-012-0008-y
pubmed: 23247825
Jovanović B. Batch cultivation of Trichoderma reesei. In: Mach-Aigner AR, Martzy R, editors. Trichoderma reesei methods in molecular biology, vol. 2234. Humana: New york; 2021. p. 113–8.
doi: 10.1007/978-1-0716-1048-0_10
Carly F, Fickers P. Erythritol production by yeasts: a snapshot of current knowledge. Yeast. 2018;35(7):455–63. https://doi.org/10.1002/yea.3306 .
doi: 10.1002/yea.3306
pubmed: 29322598
Sar T, Harirchi S, Ramezani M, Bulkan G, Akbas MY, Pandey A, et al. Potential utilization of dairy industries by-products and wastes through microbial processes: a critical review. Sci Total Environ. 2022;810: 152253. https://doi.org/10.1016/j.scitotenv.2021.152253 .
doi: 10.1016/j.scitotenv.2021.152253
pubmed: 34902412
Knežević K, Daza-Serna L, Mach-Aigner AR, Mach RL, Friedl A, Krampe J, et al. Investigation of ion-exchange membranes and erythritol concentration for the desalination of erythritol culture broth by electrodialysis. Chem Eng Process Process Intensif. 2023;192: 109494. https://doi.org/10.1016/j.cep.2023.109494 .
doi: 10.1016/j.cep.2023.109494
Moon HJ, Jeya M, Kim IW, Lee JK. Biotechnological production of erythritol and its applications. Appl Microbiol Biotechnol. 2010;86(4):1017–25. https://doi.org/10.1007/s00253-010-2496-4 .
doi: 10.1007/s00253-010-2496-4
pubmed: 20186409
Lin SJ, Wen CY, Liau JC, Chu WS. Screening and production of erythritol by newly isolated osmophilic yeast-like fungi. Process Biochem. 2001;36:1249–58. https://doi.org/10.1016/S0032-9592(01)00169-8 .
doi: 10.1016/S0032-9592(01)00169-8
Carly F, Vandermies M, Telek S, Steels S, Thomas S, Nicaud JM, et al. Enhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering. Metab Eng. 2017;42:19–24. https://doi.org/10.1016/j.ymben.2017.05.002 .
doi: 10.1016/j.ymben.2017.05.002
pubmed: 28545807
Carly F, Steels S, Telek S, Vandermies M, Nicaud JM, Fickers P. Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose. Bioresour Technol. 2018;247:963–9. https://doi.org/10.1016/j.biortech.2017.09.168 .
doi: 10.1016/j.biortech.2017.09.168
pubmed: 30060436
Rymowicz W, Rywińska A, Marcinkiewicz M. High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica. Biotechnol Lett. 2009;31(3):377–80. https://doi.org/10.1007/s10529-008-9884-1 .
doi: 10.1007/s10529-008-9884-1
pubmed: 19037599
Guo J, Li J, Chen Y, Guo X, Xiao D. Improving erythritol production of Aureobasidium pullulans from xylose by mutagenesis and medium optimization. Appl Biochem Biotechnol. 2016;180(4):717–27. https://doi.org/10.1007/s12010-016-2127-3 .
doi: 10.1007/s12010-016-2127-3
pubmed: 27402193
Yu JH, Lee DH, Oh YJ, Han KC, Ryu YW, Seo JH. Selective utilization of fructose to glucose by Candida magnoliae, an erythritol producer. Appl Biochem Biotechnol. 2006;131(1–3):870–9. https://doi.org/10.1385/ABAB:131:1:870 .
doi: 10.1385/ABAB:131:1:870
pubmed: 18563661
Soerjawinata W, Kockler I, Wommer L, Frank R, Schüffler A, Schirmeister T, et al. Novel bioreactor internals for the cultivation of spore-forming fungi in pellet form. Eng Life Sci. 2022;22(7):474–83. https://doi.org/10.1002/elsc.202100094 .
doi: 10.1002/elsc.202100094
pubmed: 35865648
pmcid: 9288991
Jordan P, Choe JY, Boles E, Oreb M. Hxt13, Hxt15, Hxt16 and Hxt17 from Saccharomyces cerevisiae represent a novel type of polyol transporters. Sci Rep. 2016;6:23502. https://doi.org/10.1038/srep23502 .
doi: 10.1038/srep23502
pubmed: 26996892
pmcid: 4800717
Geddes BA, Pickering BS, Poysti NJ, Collins H, Yudistira H, Oresnik IJ. A locus necessary for the transport and catabolism of erythritol in Sinorhizobium meliloti. Microbiology. 2010;156(Pt 10):2970–81. https://doi.org/10.1099/mic.0.041905-0 .
doi: 10.1099/mic.0.041905-0
pubmed: 20671019
Ortiz SR, Heinz A, Hiller K, Field MS. Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells. Front Nutr. 2022;9: 953056. https://doi.org/10.3389/fnut.2022.953056 .
doi: 10.3389/fnut.2022.953056
pubmed: 36276829
pmcid: 9582529
Battaglia E, Zhou M, de Vries RP. The transcriptional activators AraR and XlnR from Aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes. Res Microbiol. 2014;165:531–40. https://doi.org/10.1016/j.resmic.2014.07.013 .
doi: 10.1016/j.resmic.2014.07.013
pubmed: 25086261
Masi A, Mach RL, Mach-Aigner AR. The pentose phosphate pathway in industrially relevant fungi: crucial insights for bioprocessing. Appl Microbiol Biotechnol. 2021;105:4017–31. https://doi.org/10.1007/s00253-021-11314-x .
doi: 10.1007/s00253-021-11314-x
pubmed: 33950280
pmcid: 8140973
Hankinson O, Cove DJ. Regulation of pentose phosphate pathway in fungus Aspergillus nidulans. Biochem J. 1972;127:18–9. https://doi.org/10.1042/bj1270018pb .
doi: 10.1042/bj1270018pb
Hankinson O. Mutants of the pentose phosphate pathway in Aspergillus nidulans. J Bacteriol. 1974;117:1121–30.
doi: 10.1128/jb.117.3.1121-1130.1974
pubmed: 4591946
pmcid: 246592
Osmond CB, Ap RT. Control of the pentose-phosphate pathway in yeast. Biochim Biophys Acta. 1969;184:35–42. https://doi.org/10.1016/0304-4165(69)90095-6 .
doi: 10.1016/0304-4165(69)90095-6
pubmed: 5791114
Witkowski M, Nemet I, Alamri H, Wilcox J, Gupta N, Nimer N, et al. The artificial sweetener erythritol and cardiovascular event risk. Nat Med. 2023;29(3):710–8. https://doi.org/10.1038/s41591-023-02223-9 .
doi: 10.1038/s41591-023-02223-9
pubmed: 36849732
pmcid: 10334259
Hootman KC, Trezzi JP, Kraemer L, Burwell LS, Dong X, Guertin KA, et al. Erythritol is a pentose-phosphate pathway metabolite and associated with adiposity gain in young adults. Proc Natl Acad Sci USA. 2017;114(21):E4233–40. https://doi.org/10.1073/pnas.1620079114 .
doi: 10.1073/pnas.1620079114
pubmed: 28484010
pmcid: 5448202
Rebholz CM, Yu B, Zheng Z, Chang P, Tin A, Köttgen A, et al. Serum metabolomic profile of incident diabetes. Diabetologia. 2018;61(5):1046–54. https://doi.org/10.1007/s00125-018-4573-7 .
doi: 10.1007/s00125-018-4573-7
pubmed: 29556673
pmcid: 5878141