A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain.
Animals
Perilipin-2
/ metabolism
Lipid Droplets
/ metabolism
Brain
/ metabolism
Mice
Neurons
/ metabolism
Gene Knock-In Techniques
Mice, Transgenic
Female
Luminescent Proteins
/ metabolism
Male
Astrocytes
/ metabolism
Diet, High-Fat
Mice, Inbred C57BL
Neural Stem Cells
/ metabolism
Microglia
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 Jun 2024
28 Jun 2024
Historique:
received:
20
07
2022
accepted:
03
06
2024
medline:
29
6
2024
pubmed:
29
6
2024
entrez:
28
6
2024
Statut:
epublish
Résumé
Lipid droplets (LDs) are dynamic lipid storage organelles. They are tightly linked to metabolism and can exert protective functions, making them important players in health and disease. Most LD studies in vivo rely on staining methods, providing only a snapshot. We therefore developed a LD-reporter mouse by labelling the endogenous LD coat protein perilipin 2 (PLIN2) with tdTomato, enabling staining-free fluorescent LD visualisation in living and fixed tissues and cells. Here we validate this model under standard and high-fat diet conditions and demonstrate that LDs are highly abundant in various cell types in the healthy brain, including neurons, astrocytes, ependymal cells, neural stem/progenitor cells and microglia. Furthermore, we also show that LDs are abundant during brain development and can be visualized using live imaging of embryonic slices. Taken together, our tdTom-Plin2 mouse serves as a novel tool to study LDs and their dynamics under both physiological and diseased conditions in all tissues expressing Plin2.
Identifiants
pubmed: 38942786
doi: 10.1038/s41467-024-49449-w
pii: 10.1038/s41467-024-49449-w
doi:
Substances chimiques
Perilipin-2
0
Plin2 protein, mouse
0
Luminescent Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5489Subventions
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 31003A_175570
Informations de copyright
© 2024. The Author(s).
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