Metabolomics analysis of the lactobacillus plantarum ATCC 14917 response to antibiotic stress.
Lactobacillus plantarum
/ metabolism
Metabolomics
Anti-Bacterial Agents
/ pharmacology
Ampicillin
/ pharmacology
Doxycycline
/ pharmacology
Reactive Oxygen Species
/ metabolism
Purines
/ metabolism
Stress, Physiological
/ drug effects
Metabolic Networks and Pathways
/ drug effects
Adenosine Diphosphate
/ metabolism
Humans
Lactobacillus plantarum ATCC14917
Antibiotics
Metabolomics
Probiotic protection
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
28 Jun 2024
28 Jun 2024
Historique:
received:
18
12
2023
accepted:
18
06
2024
medline:
29
6
2024
pubmed:
29
6
2024
entrez:
29
6
2024
Statut:
epublish
Résumé
Lactobacillus plantarum has been found to play a significant role in maintaining the balance of intestinal flora in the human gut. However, it is sensitive to commonly used antibiotics and is often incidentally killed during treatment. We attempted to identify a means to protect L. plantarum ATCC14917 from the metabolic changes caused by two commonly used antibiotics, ampicillin, and doxycycline. We examined the metabolic changes under ampicillin and doxycycline treatment and assessed the protective effects of adding key exogenous metabolites. Using metabolomics, we found that under the stress of ampicillin or doxycycline, L. plantarum ATCC14917 exhibited reduced metabolic activity, with purine metabolism a key metabolic pathway involved in this change. We then screened the key biomarkers in this metabolic pathway, guanine and adenosine diphosphate (ADP). The exogenous addition of each of these two metabolites significantly reduced the lethality of ampicillin and doxycycline on L. plantarum ATCC14917. Because purine metabolism is closely related to the production of reactive oxygen species (ROS), the results showed that the addition of guanine or ADP reduced intracellular ROS levels in L. plantarum ATCC14917. Moreover, the killing effects of ampicillin and doxycycline on L. plantarum ATCC14917 were restored by the addition of a ROS accelerator in the presence of guanine or ADP. The metabolic changes of L. plantarum ATCC14917 under antibiotic treatments were determined. Moreover, the metabolome information that was elucidated can be used to help L. plantarum cope with adverse stress, which will help probiotics become less vulnerable to antibiotics during clinical treatment.
Sections du résumé
BACKGROUND
BACKGROUND
Lactobacillus plantarum has been found to play a significant role in maintaining the balance of intestinal flora in the human gut. However, it is sensitive to commonly used antibiotics and is often incidentally killed during treatment. We attempted to identify a means to protect L. plantarum ATCC14917 from the metabolic changes caused by two commonly used antibiotics, ampicillin, and doxycycline. We examined the metabolic changes under ampicillin and doxycycline treatment and assessed the protective effects of adding key exogenous metabolites.
RESULTS
RESULTS
Using metabolomics, we found that under the stress of ampicillin or doxycycline, L. plantarum ATCC14917 exhibited reduced metabolic activity, with purine metabolism a key metabolic pathway involved in this change. We then screened the key biomarkers in this metabolic pathway, guanine and adenosine diphosphate (ADP). The exogenous addition of each of these two metabolites significantly reduced the lethality of ampicillin and doxycycline on L. plantarum ATCC14917. Because purine metabolism is closely related to the production of reactive oxygen species (ROS), the results showed that the addition of guanine or ADP reduced intracellular ROS levels in L. plantarum ATCC14917. Moreover, the killing effects of ampicillin and doxycycline on L. plantarum ATCC14917 were restored by the addition of a ROS accelerator in the presence of guanine or ADP.
CONCLUSIONS
CONCLUSIONS
The metabolic changes of L. plantarum ATCC14917 under antibiotic treatments were determined. Moreover, the metabolome information that was elucidated can be used to help L. plantarum cope with adverse stress, which will help probiotics become less vulnerable to antibiotics during clinical treatment.
Identifiants
pubmed: 38943061
doi: 10.1186/s12866-024-03385-3
pii: 10.1186/s12866-024-03385-3
doi:
Substances chimiques
Anti-Bacterial Agents
0
Ampicillin
7C782967RD
Doxycycline
N12000U13O
Reactive Oxygen Species
0
Purines
0
Adenosine Diphosphate
61D2G4IYVH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
229Informations de copyright
© 2024. The Author(s).
Références
König H, Fröhlich J. Biology of microorganisms on grapes, in must and in wine. Chapter 1 Lactic acid bacteria. Heidelberg: Springer. 2017:p. 3–41.
De Vries MC, Vaughan EE, Kleerebezem M, de Vos WM. Lactobacillus plantarum - survival, functional and potential probiotic properties in the human intestinal tract. Int Dairy J. 2006;16(9):1018–28.
doi: 10.1016/j.idairyj.2005.09.003
Filippis FD, Pasolli E, Ercolini D. The food-gut axis: lactic acid bacteria and their link to food, the gut microbiome and human health. FEMS Microbiol Rev. 2020;44(4):454–89.
pubmed: 32556166
pmcid: 7391071
doi: 10.1093/femsre/fuaa015
Arasu MV, Al-Dhabi NA, Ilavenil S, Choi KC, Srigopalram S. In vitro importance of probiotic Lactobacillus plantarum related to medical field. Saudi journal of biological sciences. 2016;23(1):S6–10.
pubmed: 26858567
doi: 10.1016/j.sjbs.2015.09.022
Yan ZW, Liu ZZ, Ma Y, Yang Z, Liu G, Fang J. Effects of Lactobacillus plantarum and Weissella viridescens on the gut microbiota and serum metabolites of mice with antibiotic-associated diarrhea. Nutrients. 2023;15(21):4603.
pubmed: 37960257
pmcid: 10648191
doi: 10.3390/nu15214603
Yue Y, He ZJ, Zhou YH, Ross RP, Stanton C, Zhao JX, et al. Lactobacillus plantarum relieves diarrhea caused by enterotoxin-producing Escherichia coli through inflammation modulation and gut microbiota regulation. Food Funct. 2020;11(12):10362–74.
pubmed: 33220669
doi: 10.1039/D0FO02670K
Zhao K, Qiu L, He Y, Tao XY, Zhang ZH, Wei H. Alleviation syndrome of high-cholesterol-diet-induced hypercholesterolemia in mice by intervention with Lactiplantibacillus plantarum WLPL21 via regulation of cholesterol metabolism and transportation as well as gut microbiota. Nutrients. 2023;15(11):2600.
pubmed: 37299563
pmcid: 10255518
doi: 10.3390/nu15112600
Ren R, Zhao AQ, Chen L, Wu S, Hung WL, Wang B. Therapeutic effect of Lactobacillus plantarum JS19 on mice with dextran sulfate sodium induced acute and chronic ulcerative colitis. J Sci Food Agric. 2023;103(8):4143–56.
pubmed: 36573836
doi: 10.1002/jsfa.12414
Qin SK, Wang YL, Yang MJ, Wang PP, Iqbal MI, Li JQ, et al. Lactobacillus plantarum A3 attenuates ulcerative colitis by modulating gut microbiota and metabolism. Animal diseases. 2023;3(1):16.
doi: 10.1186/s44149-023-00073-z
Sun MY, Liu WW, Song YL, Tuo YF, Mu GQ, Ma FL. The effects of Lactobacillus plantarum-12 crude exopolysaccharides on the cell proliferation and apoptosis of human colon cancer (HT-29) cells. Probiotics and antimicrobial proteins. 2021;13:413–21.
pubmed: 32844363
doi: 10.1007/s12602-020-09699-8
Stefańska I, Kwiecień E, Jóźwiak-Piasecka K, Garbowska M, Binek M, Rzewuska M. Antimicrobial susceptibility of lactic acid bacteria strains of potential use as feed additives-the basic safety and usefulness criterion. Frontiers in veterinary science. 2021;8: 687071.
pubmed: 34277757
pmcid: 8281277
doi: 10.3389/fvets.2021.687071
Anisimova EA, Yarullina DR. Antibiotic resistance of Lactobacillus strains. Curr Microbiol. 2019;76(12):1407–16.
pubmed: 31555856
doi: 10.1007/s00284-019-01769-7
Çataloluk O, Gogebakan B. Presence of drug resistance in intestinal lactobacilli of dairy and human origin in Turkey. FEMS Microbiol Lett. 2004;236(1):7–12.
pubmed: 15212784
doi: 10.1111/j.1574-6968.2004.tb09620.x
Huys G, D’Haene K, Collard JM, Swings J. Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl Environ Microbiol. 2004;70(3):1555–62.
pubmed: 15006778
pmcid: 368340
doi: 10.1128/AEM.70.3.1555-1562.2004
Lam AK, Panlilio H, Pusavat J, Wouters CL, Moen EL, Neel AJ, et al. Low-molecular-weight branched polyethylenimine potentiates ampicillin against MRSA biofilms. ACS Med Chem Lett. 2020;11(4):473–8.
pubmed: 32292552
pmcid: 7153015
doi: 10.1021/acsmedchemlett.9b00595
Akbar N, Aslam Z, Siddiqui R, Shah MR, Khan NA. Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules. AMB Express. 2021;11:1–16.
doi: 10.1186/s13568-021-01261-1
Rusu A, Buta EL. The development of third-generation tetracycline antibiotics and new perspectives. Pharmaceutics. 2021;13(12):2085.
pubmed: 34959366
pmcid: 8707899
doi: 10.3390/pharmaceutics13122085
LaPlante KL, Dhand A, Wright K, Lauterio M. Re-establishing the utility of tetracycline-class antibiotics for current challenges with antibiotic resistance. Ann Med. 2022;54(1):1686–700.
pubmed: 35723082
pmcid: 9225766
doi: 10.1080/07853890.2022.2085881
Hidron AI, Edwards JR, Patel J, Horan TC, Sievert DM, Pollock DA, et al. Antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006–2007. Infect Control Hosp Epidemiol. 2008;29(11):996–1011.
pubmed: 18947320
doi: 10.1086/591861
Willems RJ, Van Schaik W. Transition of Enterococcus faecium from commensal organism to nosocomial pathogen. Future Microbiol. 2009;4(9):1125–35.
pubmed: 19895216
doi: 10.2217/fmb.09.82
Zhang XL, Paganelli FL, Bierschenk D, Kuipers A, Bonten MJ, Willems RJ, et al. Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium. PLoS Genet. 2012;8(6): e1002804.
pubmed: 22761597
pmcid: 3386183
doi: 10.1371/journal.pgen.1002804
Li M, Liu Q, Teng Y, Ou L, Xi Y, Chen S, et al. The resistance mechanism of Escherichia coli induced by ampicillin in laboratory. Infection and drug resistance. 2019;12:2853–63.
pubmed: 31571941
pmcid: 6750165
doi: 10.2147/IDR.S221212
Gu L, Li SL, He Y, Chen Y, Jiang YZ, Peng Y, et al. Bismuth, rabeprazole, amoxicillin, and doxycycline as first-line Helicobacter pylori therapy in clinical practice: A pilot study. Helicobacter. 2019;24(4): e12594.
pubmed: 31119830
doi: 10.1111/hel.12594
Lange K, Buerger M, Stallmach A, Bruns T. Effects of antibiotics on gut microbiota. Dig Dis. 2016;34(3):260–8.
pubmed: 27028893
doi: 10.1159/000443360
Darmastuti A, Hasan PN, Wikandari R, Utami T, Rahayu ES, Suroto DA. Adhesion properties of Lactobacillus plantarum Dad-13 and Lactobacillus plantarum Mut-7 on Sprague Dawley rat intestine. Microorganisms. 2021;9(11):2336.
pubmed: 34835461
pmcid: 8625926
doi: 10.3390/microorganisms9112336
Dörries K, Schlueter R, Lalk M. Impact of antibiotics with various target sites on the metabolome of Staphylococcus aureus. Antimicrob Agents Chemother. 2014;58(12):7151–63.
pubmed: 25224006
pmcid: 4249544
doi: 10.1128/AAC.03104-14
Vincent IM, Ehmann DE, Mills SD, Perros M, Barrett MP. Untargeted metabolomics to ascertain antibiotic modes of action. Antimicrob Agents Chemother. 2016;60(4):2281–91.
pubmed: 26833150
pmcid: 4808186
doi: 10.1128/AAC.02109-15
Schelli K, Zhong F, Zhu J. Comparative metabolomics revealing Staphylococcus aureus metabolic response to different antibiotics. Microb Biotechnol. 2017;10(6):1764–74.
pubmed: 28815967
pmcid: 5658637
doi: 10.1111/1751-7915.12839
Peng B, Li H, Peng XX. Call for next-generation drugs that remove the uptake barrier to combat antibiotic resistance. Drug Discovery Today. 2023;28(10): 103753.
pubmed: 37640151
doi: 10.1016/j.drudis.2023.103753
Stokes JM, Lopatkin AJ, Lobritz MA, Collins JJ. Bacterial metabolism and antibiotic efficacy. Cell Metab. 2019;30(2):251–9.
pubmed: 31279676
pmcid: 6990394
doi: 10.1016/j.cmet.2019.06.009
Su YB, Peng B, Han Y, Li H, Peng XX. Fructose restores susceptibility of multidrug-resistant Edwardsiella tarda to kanamycin. J Proteome Res. 2015;14(3):1612–20.
pubmed: 25675328
doi: 10.1021/pr501285f
Hassan A, Luqman A, Zhang K, Ullah M, Din AU, Liao XL, et al. Impact of probiotic Lactobacillus plantarum ATCC 14917 on atherosclerotic plaque and its mechanism. World Journal of Microbiology Biotechnology. 2024;40(7):198.
pubmed: 38727952
doi: 10.1007/s11274-024-04010-1
Hassan A, Din AU, Zhu Y, Zhang K, Li TH, Wang Y, et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE−/−mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020;104:6337–50.
pubmed: 32472174
doi: 10.1007/s00253-020-10693-x
Mantzourani I, Kazakos S, Terpou A, Alexopoulos A, Bezirtzoglou E, Bekatorou A, et al. Potential of the probiotic Lactobacillus plantarum ATCC 14917 strain to produce functional fermented pomegranate juice. Foods. 2018;8(1):4.
pubmed: 30583502
pmcid: 6352242
doi: 10.3390/foods8010004
Klare I, Konstabel C, Müller-Bertling S, Reissbrodt R, Huys G, Vancanneyt M, et al. Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria. Appl Environ Microbiol. 2005;71(12):8982–6.
pubmed: 16332905
pmcid: 1317481
doi: 10.1128/AEM.71.12.8982-8986.2005
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163–75.
pubmed: 18274517
doi: 10.1038/nprot.2007.521
Knudsen GM, Fromberg A, Ng Y, Gram L. Sublethal concentrations of antibiotics cause shift to anaerobic metabolism in Listeria monocytogenes and induce phenotypes linked to antibiotic tolerance. Front Microbiol. 2016;7:1091.
pubmed: 27462313
pmcid: 4940397
doi: 10.3389/fmicb.2016.01091
Peng B, Su YB, Li H, Han Y, Guo C, Tian YM, et al. Exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. Cell Metab. 2015;21(2):249–62.
pubmed: 25651179
doi: 10.1016/j.cmet.2015.01.008
Ye JZ, Su YB, Peng XX, Li H. Reactive oxygen species-related ceftazidime resistance is caused by the pyruvate cycle perturbation and reverted by Fe
pubmed: 33995314
pmcid: 8113649
doi: 10.3389/fmicb.2021.654783
Smith CA, Want EJ, O’Maille G, Abagyan R, Siuzdak G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Anal Chem. 2006;78(3):779–87.
pubmed: 16448051
doi: 10.1021/ac051437y
Peng B, Ma YM, Zhang JY, Li H. Metabolome strategy against Edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias. Fish Shellfish Immunol. 2015;45(2):869–76.
pubmed: 26057462
doi: 10.1016/j.fsi.2015.06.004
Heunis T, Deane S, Smit S, Dicks LM. Proteomic profiling of the acid stress response in Lactobacillus plantarum 423. J Proteome Res. 2014;13(9):4028–39.
pubmed: 25068841
doi: 10.1021/pr500353x
Wilson WR, Cockerill FR. Tetracyclines, chloramphenicol, erythromycin, and clindamycin. Mayo Clin Proc. 1987;62(10):906–15.
pubmed: 3657308
doi: 10.1016/S0025-6196(12)65047-2
Kapoor G, Saigal S, Elongavan A. Action and resistance mechanisms of antibiotics: A guide for clinicians. J Anaesthesiol Clin Pharmacol. 2017;33(3):300.
pubmed: 29109626
pmcid: 5672523
doi: 10.4103/joacp.JOACP_349_15
Kowalska-Krochmal B, Dudek-Wicher R. The minimum inhibitory concentration of antibiotics: Methods, interpretation, clinical relevance. Pathogens. 2021;10(2):165.
pubmed: 33557078
pmcid: 7913839
doi: 10.3390/pathogens10020165
Lebeaux D, Ghigo JM, Beloin C. Biofilm-related infections: bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics. Microbiol Mol Biol Rev. 2014;78(3):510–43.
pubmed: 25184564
pmcid: 4187679
doi: 10.1128/MMBR.00013-14
Laverty G, Gorman SP, Gilmore BF. Biomolecular mechanisms of staphylococcal biofilm formation. Future Microbiol. 2013;8(4):509–24.
pubmed: 23534362
doi: 10.2217/fmb.13.7
Nalbant D, Reeder JA, Li P, O’Sullivan CT, Rogers WK, An G. Development and validation of a simple and sensitive LC-MS/MS method for quantification of ampicillin and sulbactam in human plasma and its application to a clinical pharmacokinetic study. J Pharm Biomed Anal. 2021;196: 113899.
pubmed: 33508765
doi: 10.1016/j.jpba.2021.113899
Singh S, Khanna D, Kalra S. Minocycline and doxycycline: more than antibiotics. Curr Mol Pharmacol. 2021;14(6):1046–65.
pubmed: 33568043
doi: 10.2174/1874467214666210210122628
Lee H, Choi YY, Sohn YJ, Kim YK, Han MS, Yun KW, et al. Clinical efficacy of doxycycline for treatment of macrolide-resistant Mycoplasma pneumoniae pneumonia in children. Antibiotics. 2021;10(2):192.
pubmed: 33671151
pmcid: 7921960
doi: 10.3390/antibiotics10020192
Zhou XJ, Zhang ZF, Suo YJ, Cui Y, Zhang F, Shi CL, et al. Effect of sublethal concentrations of ceftriaxone on antibiotic susceptibility of multiple antibiotic-resistant Salmonella strains. FEMS microbiology letters. 2019;366(2):283.
doi: 10.1093/femsle/fny283
Byun KH, Han SH, Choi MW, Park SH, Ha SD. Effect of sublethal concentrations of bactericidal antibiotics on mutation frequency and stress response of Listeria monocytogenes. Food Res Int. 2022;151: 110903.
pubmed: 34980420
doi: 10.1016/j.foodres.2021.110903
Zhang Q, Cheng JP, Xin Q. Effects of tetracycline on developmental toxicity and molecular responses in zebrafish (Danio rerio) embryos. Ecotoxicology. 2015;24:707–19.
pubmed: 25588674
doi: 10.1007/s10646-015-1417-9
Bush K, Bradford PA. β-Lactams and β-lactamase inhibitors: an overview. Cold Spring Harb Perspect Med. 2016;6(8): a025247.
pubmed: 27329032
pmcid: 4968164
doi: 10.1101/cshperspect.a025247
Griffin MO, Fricovsky E, Ceballos G, Villarrea F. Tetracyclines: a pleitropic family of compounds with promising therapeutic properties. Review of the literature. American Journal of Physiology-Cell Physiology. 2010;299(3):539–48.
doi: 10.1152/ajpcell.00047.2010
Zhao XL, Drlica K. Reactive oxygen species and the bacterial response to lethal stress. Curr Opin Microbiol. 2014;21:1–6.
pubmed: 25078317
doi: 10.1016/j.mib.2014.06.008
Van Acker H, Coenye T. The role of reactive oxygen species in antibiotic-mediated killing of bacteria. Trends Microbiol. 2017;25(6):456–66.
pubmed: 28089288
doi: 10.1016/j.tim.2016.12.008
Lu J, Jin M, Nguyen SH, Mao LK, Li J, Coin LJM, et al. Non-antibiotic antimicrobial triclosan induces multiple antibiotic resistance through genetic mutation. Environ Int. 2018;118:257–65.
pubmed: 29902774
doi: 10.1016/j.envint.2018.06.004
Pullaguri N, Umale A, Bhargava A. Neurotoxic mechanisms of triclosan: The antimicrobial agent emerging as a toxicant. J Biochem Mol Toxicol. 2023;37(2): e23244.
pubmed: 36353933
doi: 10.1002/jbt.23244
Kaźmierczak-Siedlecka K, Daca A, Folwarski M, Witkowski JM, Bryl E, Makarewicz W. The role of Lactobacillus plantarum 299v in supporting treatment of selected diseases. Central european journal of immunology. 2020;45(4):488–93.
pubmed: 33613097
doi: 10.5114/ceji.2020.101515
Mbye M, Baig MA, AbuQamar SF, El-Tarabily KA, Obaid RS, Osaili TM, et al. Updates on understanding of probiotic lactic acid bacteria responses to environmental stresses and highlights on proteomic analyses. Comprehensive Reviews in Food Science and Food Safety. 2020;19(3):1110–24.
pubmed: 33331686
doi: 10.1111/1541-4337.12554
Pieterse B, Leer RJ, Schuren FH, van der Werf MJ. Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling. Microbiology. 2005;151(12):3881–94.
pubmed: 16339934
doi: 10.1099/mic.0.28304-0
Huang RH, Pan MF, Wan CX, Shah NP, Tao XY, Wei H. Physiological and transcriptional responses and cross protection of Lactobacillus plantarum ZDY2013 under acid stress. J Dairy Sci. 2016;99(2):1002–10.
pubmed: 26627851
doi: 10.3168/jds.2015-9993
Wang WW, He JY, Pan DD, Wu Z, Guo YX, Zeng XQ, et al. Metabolomics analysis of Lactobacillus plantarum ATCC 14917 adhesion activity under initial acid and alkali stress. PLoS ONE. 2018;13(5): e0196231.
pubmed: 29795550
pmcid: 5967736
doi: 10.1371/journal.pone.0196231
Wu LY, Wang WW, Wu Z, Pan DD, Zeng XQ, Guo YX, et al. Effect of acid and alkali stress on extracellular metabolite profile of Lactobacillus plantarum ATCC 14917. J Basic Microbiol. 2020;60(8):722–9.
pubmed: 32452552
doi: 10.1002/jobm.202000203
Mantena RK, Wijbur OL, Vindurampulle C, Bennett‐Wood VR, Walduc A, Drummond GR, et al. Reactive oxygen species are the major antibacterials against Salmonella Typhimurium purine auxotrophs in the phagosome of RAW 264.7 cells. Cellular microbiology. 2008;10(5):1058–73.
pubmed: 18067606
doi: 10.1111/j.1462-5822.2007.01105.x
Shaffer CL, Zhang EW, Dudley AG, Guckes KR, Breland EJ, et al. Purine biosynthesis metabolically constrains intracellular survival of uropathogenic Escherichia coli. Infection and immunity. 2017;85(1):00471–16.
doi: 10.1128/IAI.00471-16
Yang JH, Wright SN, Hamblin M, McCloskey D, Alcantar MA, Schrübbers L, et al. A white-box machine learning approach for revealing antibiotic mechanisms of action. Cell. 2019;177(6):1649–61.
pubmed: 31080069
pmcid: 6545570
doi: 10.1016/j.cell.2019.04.016
Chen L, Liu R, Li SY, Wu M, Yu H, Ge QF. Metabolism of hydrogen peroxide by Lactobacillus plantarum NJAU-01: A proteomics study. Food Microbiol. 2023;112: 104246.
pubmed: 36906310
doi: 10.1016/j.fm.2023.104246
Luo X, Li M, Zhang HN, Yan DL, Ji SQ, Wu R, et al. Comparative proteomic analysis of three Lactobacillus plantarum strains under salt stress by iTRAQ. J Sci Food Agric. 2021;101(8):3457–71.
pubmed: 33270231
doi: 10.1002/jsfa.10976
Zhai QX, Xiao Y, Narbad A, Chen W. Comparative metabolomic analysis reveals global cadmium stress response of Lactobacillus plantarum strains. Metallomics. 2018;10(8):1065–77
pubmed: 29998247
doi: 10.1039/C8MT00095F
Jiang M, Su YB, Ye JZ, Li H, Kuang SF, Wu JH, et al. Ampicillin-controlled glucose metabolism manipulates the transition from tolerance to resistance in bacteria. Science advances. 2023;9(10):8582.
doi: 10.1126/sciadv.ade8582
Hong YZ, Zeng J, Wang XH, Drlica K, Zhao XL. Post-stress bacterial cell death mediated by reactive oxygen species. Proc Natl Acad Sci. 2019;116(20):10064–71.
pubmed: 30948634
pmcid: 6525477
doi: 10.1073/pnas.1901730116
Liu Y, Tong ZW, Shi JR, Jia YQ, Deng T, Wang ZQ. Reversion of antibiotic resistance in multidrug-resistant pathogens using non-antibiotic pharmaceutical benzydamine. Communications biology. 2021;4(1):1328.
pubmed: 34824393
pmcid: 8616900
doi: 10.1038/s42003-021-02854-z
Wang YH, Su JF, Zhou ZY, Yang J, Liu WJ, Zhang YF, et al. Baicalein resensitizes multidrug-resistant Gram-negative pathogens to doxycycline. Microbiology spectrum. 2023;11(3):e04702-e4722.
pubmed: 37070985
pmcid: 10269726
Zhao XL, Chen ZG, Yang TC, Jiang M, Wang J, Cheng XZ, et al. Glutamine promotes antibiotic uptake to kill multidrug-resistant uropathogenic bacteria. Science Translational Medicine. 2021;13(625):0716.
doi: 10.1126/scitranslmed.abj0716
Cheng ZX, Guo C, Chen ZG, Yang TC, Zhang JY, Wang J, et al. Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing. Nat Commun. 2019;10(1):3325.
pubmed: 31346171
pmcid: 6658569
doi: 10.1038/s41467-019-11129-5