The effects of Quercetin on wound healing in the human umbilical vein endothelial cells.
FGF
Human umbilical vein endothelial cells (HUVECs)
Quercetin
VEGF
Wound healing
Journal
Cell and tissue banking
ISSN: 1573-6814
Titre abrégé: Cell Tissue Bank
Pays: Netherlands
ID NLM: 100965121
Informations de publication
Date de publication:
28 Jun 2024
28 Jun 2024
Historique:
received:
10
12
2022
accepted:
12
06
2024
medline:
30
6
2024
pubmed:
30
6
2024
entrez:
29
6
2024
Statut:
aheadofprint
Résumé
An injury that affects the integrity of the skin, either inside or externally, is called a wound. Damaged tissue is repaired by a set of cellular and molecular mechanisms known as wound healing. Quercetin, a naturally occurring flavonoid, may hasten the healing of wounds. The study's objective was to investigate any potential impacts of quercetin on the wound-healing process. Human umbilical vein endothelial cells (HUVECs) were treated to varying dose ranges of quercetin (5-320 nM) for 24 and 48 h. Cultured cells were evaluated by using the MTT analysis, wound scratch assay and vascular tube formation. Furthermore the gene expression of VEGF and FGF were evaluated by qRT-PCR to determine the effects of quercetin on angiogenezis and wound repair. Positive effects of quercetin on cellular viability were demonstrated by the MTT experiment. In HUVECs quercetin promoted tube formation, migration, and proliferation while also averting wound breakage. Moreover, quercetin increased the expression of the FGF and VEGF genes, which aid in the healing of wounds in HUVECs. Quercetin may be bioactive molecule that successfully speeds up wound healing by regulating the vasculogenezis and healing cells.
Identifiants
pubmed: 38944663
doi: 10.1007/s10561-024-10144-1
pii: 10.1007/s10561-024-10144-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Ahmad A, Nawaz MI (2022) Molecular mechanism of VEGF and its role in pathological angiogenesis. J Cell Biochem 123(12):1938–1965. https://doi.org/10.1002/jcb.30344
doi: 10.1002/jcb.30344
pubmed: 36288574
Anand David AV, Arulmoli R, Parasuraman S (2016) Overviews of biological importance of quercetin: a bioactive flavonoid. Phcog Rev 10:84–89
doi: 10.4103/0973-7847.194044
pubmed: 28082789
pmcid: 5214562
Ansell DM, Izeta A (2015) Pericytes in wound healing: friend or foe? Exp Dermatol 24(11):833–834. https://doi.org/10.1111/exd.12782
doi: 10.1111/exd.12782
pubmed: 26121283
Arango Duque G, Descoteaux A (2014) Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol 7(5):491. https://doi.org/10.3389/fimmu.2014.00491
doi: 10.3389/fimmu.2014.00491
Armulik A, Genové G, Betsholtz C (2011) Pericytes: developmental, physiological, and pathological perspectives, problems, and promises. Dev Cell 21(2):193–215. https://doi.org/10.1016/j.devcel.2011.07.001
doi: 10.1016/j.devcel.2011.07.001
pubmed: 21839917
Azimi-Nezhad M, Stathopoulou MG, Bonnefond A, Rancier M, Saleh A, Lamont J, Fitzgerald P, Ndiaye NC, Visvikis-Siest S (2013) Associations of vascular endothelial growth factor (VEGF) with adhesion and inflammation molecules in a healthy population. Cytokine 61(2):602–607. https://doi.org/10.1016/j.cyto.2012.10.024
doi: 10.1016/j.cyto.2012.10.024
pubmed: 23201487
Beken B, Serttas R, Yazicioglu M, Turkekul K, Erdogan S (2020) Quercetin improves inflammation, oxidative stress, and impaired wound healing in atopic dermatitis model of human keratinocytes. Pediatr Allergy Immunol Pulmonol 33(2):69–79. https://doi.org/10.1089/ped.2019.1137
doi: 10.1089/ped.2019.1137
pubmed: 34678092
pmcid: 8443267
Bhagwat S, Haytowitz DB, Holden JM (2011) USDA database for the flavonoid content of selected foods, Release 3. US Department of Agriculture, Beltsville, p 159
Bowers SLK, Kemp SS, Aguera KN, Koller GM, Forgy JC, Davis GE (2020) Defining an upstream VEGF (vascular endothelial growth factor) priming signature for downstream factor-induced endothelial cell-pericyte tube network coassembly. Arterioscler Thromb Vasc Biol 40(12):2891–2909
doi: 10.1161/ATVBAHA.120.314517
pubmed: 33086871
pmcid: 7939123
Chittasupho C, Manthaisong A, Okonogi S, Tadtong S, Samee W (2021) Effects of quercetin and curcumin combination on antibacterial, antioxidant, ın vitro wound healing and migration of human dermal fibroblast cells. Int J Mol Sci 23(1):142. https://doi.org/10.3390/ijms23010142
doi: 10.3390/ijms23010142
pubmed: 35008566
pmcid: 8745450
Doersch KM, Newell-Rogers MK (2017) The impact of quercetin on wound healing relates to changes in αV and β1 integrin expression. Exp Biol Med 242(14):1424–1431. https://doi.org/10.1177/1535370217712961
doi: 10.1177/1535370217712961
Eilken HM, Adams RH (2010) Dynamics of endothelial cell behavior in sprouting angiogenesis. Curr Opin Cell Biol 22(5):617–625. https://doi.org/10.1016/j.ceb.2010.08.010
doi: 10.1016/j.ceb.2010.08.010
pubmed: 20817428
Farooq M, Khan AW, Kim MS, Choi S (2021) The role of fibroblast growth factor (FGF) signaling in tissue repair and regeneration. Cells 10(11):3242. https://doi.org/10.3390/cells10113242
doi: 10.3390/cells10113242
pubmed: 34831463
pmcid: 8622657
Fu J, Huang J, Lin M, Xie T, You T (2020) Quercetin promotes diabetic wound healing via switching macrophages from M1 to M2 polarization. J Surg Res 246:213–223. https://doi.org/10.1016/j.jss.2019.09.011
doi: 10.1016/j.jss.2019.09.011
pubmed: 31606511
Gál P, Toporcer T, Grendel T, Vidová Z, Smetana K Jr, Dvoránková B, Gál T, Mozes S, Lenhardt L, Longauer F, Sabol M, Sabo J, Backor M (2009) Effect of Atropa belladonna L. on skin wound healing: biomechanical and histological study in rats and in vitro study in keratinocytes, 3T3 fibroblasts, and human umbilical vein endothelial cells. Wound Repair Regen 17(3):378–86. https://doi.org/10.1111/j.1524-475X.2009.00475.x
Gentile MT, Pastorino O, Bifulco M, Colucci-D’Amato L (2019) HUVEC tube-formation assay to evaluate the impact of natural products on angiogenesis. J Vis Exp. https://doi.org/10.3791/58591
doi: 10.3791/58591
pubmed: 31282895
Gopalakrishnan A, Ram M, Kumawat S, Tandan S, Kumar D (2016) Quercetin accelerated cutaneous wound healing in rats by increasing levels of VEGF and TGF-β1. Indian J Exp Biol 54(3):187–195
pubmed: 27145632
Guo S, Dipietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–29. https://doi.org/10.1177/0022034509359125
doi: 10.1177/0022034509359125
pubmed: 20139336
pmcid: 2903966
Gurtner GC, Werner S, Barrandon Y, Longaker MT (2008) Wound repair and regeneration. Nature 453(7193):314–21. https://doi.org/10.1038/nature07039
doi: 10.1038/nature07039
pubmed: 18480812
Huang CY, Ng MY, Lin T, Liao YW, Huang WS, Hsieh CW, Yu CC, Chen CJ (2024) Quercetin ameliorates advanced glycation end product-induced wound healing impairment and inflammaging in human gingival fibroblasts. J Dent Sci 19(1):268–275. https://doi.org/10.1016/j.jds.2023.04.014
doi: 10.1016/j.jds.2023.04.014
pubmed: 38303825
Hosseini A, Razavi BM, Banach M, Hosseinzadeh H (2021) Quercetin and metabolic syndrome: a review. Phytother Res 35(10):5352–5364. https://doi.org/10.1002/ptr.7144
doi: 10.1002/ptr.7144
pubmed: 34101925
Jee J-P, Pangeni R, Jha S, Byun Y, Park J (2019) Preparation and in vivo evaluation of a topical hydrogel system incorporating highly skin-permeable growth factors, quercetin, and oxygen carriers for enhanced diabetic wound-healing therapy. Int J Nanomed 14:66. https://doi.org/10.2147/IJN.S213883
doi: 10.2147/IJN.S213883
Jeong SM, Kang MJ, Choi HN, Kim JH, Kim JI (2012) Quercetin ameliorates hyperglycemia and dyslipidemia and improves antioxidant status in type 2 diabetic db/db mice. Nutr Res Pract 6(3):201–207. https://doi.org/10.4162/nrp.2012.6.3.201
doi: 10.4162/nrp.2012.6.3.201
pubmed: 22808343
pmcid: 3395784
Kant V, Jangir BL, Kumar V, Nigam A, Sharma V (2020) Quercetin accelerated cutaneous wound healing in rats by modulation of different cytokines and growth factors. Growth Factors 38(2):105–119. https://doi.org/10.1080/08977194.2020.1822830
doi: 10.1080/08977194.2020.1822830
pubmed: 32957814
Koike Y, Yozaki M, Utani A, Murota H (2020) Fibroblast growth factor 2 accelerates the epithelial-mesenchymal transition in keratinocytes during wound healing process. Sci Rep 10(1):18545. https://doi.org/10.1038/s41598-020-75584-7
doi: 10.1038/s41598-020-75584-7
pubmed: 33122782
pmcid: 7596476
Lakhanpal Parul (2007) Quercetin: a versatile flavonoid. Internet J Med Update. https://doi.org/10.4314/ijmu.v2i2.39851
doi: 10.4314/ijmu.v2i2.39851
Lammert E, Axnick J (2012) Vascular lumen formation. Cold Spring Harb Perspect Med 2(4):a006619. https://doi.org/10.1101/cshperspect.a006619
doi: 10.1101/cshperspect.a006619
pubmed: 22474612
pmcid: 3312398
Lévigne D, Modarressi A, Krause KH, Krause B, Krause B (2016) NADPH oxidase 4 deficiency leads to impaired wound repair and reduced dityrosine-crosslinking, but does not affect myofi-broblast formation. Free Radic Biol Med. 96:374–384
doi: 10.1016/j.freeradbiomed.2016.04.194
pubmed: 27140231
Liu Y, Liu Y, Deng J, Li W, Nie X (2021) Fibroblast growth factor in diabetic foot ulcer: progress and therapeutic prospects. Front Endocrinol 12:744–868. https://doi.org/10.3389/fendo.2021.744868
doi: 10.3389/fendo.2021.744868
McKay TB, Kivanany PB, Nicholas SE, Nag OK, Elliott MH, Petroll WM, Karamichos D (2022) Quercetin decreases corneal haze in vivo and influences gene expression of TGF-Beta mediators in vitro. Metabolites 12(7):626. https://doi.org/10.3390/metabo12070626
doi: 10.3390/metabo12070626
pubmed: 35888751
pmcid: 9318747
McKay TB, Emmitte KA, German C, Karamichos D (2023) Quercetin and related analogs as therapeutics to promote tissue repair. Bioengineering 10(10):1127. https://doi.org/10.3390/bioengineering10101127
doi: 10.3390/bioengineering10101127
pubmed: 37892857
pmcid: 10604618
Mehrbod P, Abdalla MA, Fotouhi F, Heidarzadeh M, Aro AO, Eloff JN, McGaw LJ, Fasina FO (2018) Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus. BMC Complement Altern Med 18(1):184. https://doi.org/10.1186/s12906-018-2246-1
doi: 10.1186/s12906-018-2246-1
pubmed: 29903008
pmcid: 6003079
Miean KH, Mohamed S (2001) Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem 49(6):3106–3112. https://doi.org/10.1021/jf000892m
doi: 10.1021/jf000892m
pubmed: 11410016
Newman DJ, Cragg GM (2020) Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. J Nat Prod 83(3):770–803. https://doi.org/10.1021/acs.jnatprod.9b01285
doi: 10.1021/acs.jnatprod.9b01285
pubmed: 32162523
Patridge E, Gareiss P, Kinch MS, Hoyer D (2016) An analysis of FDA-approved drugs: Natural products and their derivatives. Drug Discov Today 21(2):204–207. https://doi.org/10.1016/j.drudis.2015.01.009
doi: 10.1016/j.drudis.2015.01.009
pubmed: 25617672
Peter O, Victor N, Zibao G, Ming G, Samuel B, Sandra B (2008) Variation of flavonoid content among sweetpotato accessions. J Am Soc Hortic Sci 133:19–824. https://doi.org/10.21273/JASHS.133.6.819
doi: 10.21273/JASHS.133.6.819
Prakash OM, Singh R, Singh N et al (2018) Exploring the potentials of Quercetin and Kaempferol combinations along with regular antibiotics for the effective management of Methicillin-resistant Staphylococcus aureus (MRSA). World J Microbiol Biotechnol 8:6–9
Sulaiman CT, Balachandran I (2012) Total phenolics and total flavonoids in selected Indian medicinal plants. Indian J Pharm Sci 74(3):258–260. https://doi.org/10.4103/0250-474X.106069
doi: 10.4103/0250-474X.106069
pubmed: 23439764
pmcid: 3574537
Talbott HE, Mascharak S, Griffin M, Wan DC, Longaker MT (2022) Wound healing, fibroblast heterogeneity, and fibrosis. Cell Stem Cell 29(8):1161–1180
doi: 10.1016/j.stem.2022.07.006
pubmed: 35931028
pmcid: 9357250
Tonnesen MG, Feng X, Clark RA (2000) Angiogenesis in wound healing. J Investig Dermatol Symp Proc 5(1):40–46. https://doi.org/10.1046/j.1087-0024.2000.00014.x
doi: 10.1046/j.1087-0024.2000.00014.x
pubmed: 11147674
Velnar T, Bailey T, Smrkolj V (2009) The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res 37(5):1528–1542. https://doi.org/10.1177/147323000903700531
doi: 10.1177/147323000903700531
pubmed: 19930861
Veith AP, Henderson K, Spencer A, Sligar AD, Baker AB (2019) Therapeutic strategies for enhancing angiogenesis in wound healing. Adv Drug Deliv Rev 146:97–125. https://doi.org/10.1016/j.addr.2018.09.010
doi: 10.1016/j.addr.2018.09.010
pubmed: 30267742
Wang R, Lechtenberg M, Sendker J, Petereit F, Deters A, Hensel A (2013) Wound-healing plants from TCM: in vitro investigations on selected TCM plants and their influence on human dermal fibroblasts and keratinocytes. Fitoterapia 84:308–317. https://doi.org/10.1016/j.fitote.2012.12.020
doi: 10.1016/j.fitote.2012.12.020
pubmed: 23266731
Wei Y, Fu J, Wu W, Ma P, Ren L, Yi Z, Wu J (2021) Quercetin prevents oxidative stress-induced injury of periodontal ligament cells and alveolar bone loss in periodontitis. Drug Des Devel Ther 15:3509–3522. https://doi.org/10.2147/DDDT.S315249
doi: 10.2147/DDDT.S315249
pubmed: 34408403
pmcid: 8366957
Wilkinson HN, Hardman MJ (2020) Wound healing: cellular mechanisms and pathological outcomes. Open Biol 10(9):200223. https://doi.org/10.1098/rsob.200223
doi: 10.1098/rsob.200223
pubmed: 32993416
pmcid: 7536089
Xu F, Cao S, Wang C, Wang K, Wei Y, Shao X, Wang H (2019) Antimicrobial activity of flavonoids from Sedum aizoon L. against Aeromonas in culture medium and in frozen pork. Food Sci Nutr 7(10):3224–3232. https://doi.org/10.1002/fsn3.1178
doi: 10.1002/fsn3.1178
pubmed: 31660136
pmcid: 6804768