An efficient EPR spin-labeling method enables insights of conformational changes in DNA.

Electron Paramagnetic Resonance (EPR) is a powerful tool for elucidating both static and dynamic conformational alterations in macromolecules. However, to effectively utilize EPR for such investigations, the presence of paramagnetic enters, known as spin-labels, is required. The process of spin-labeling, particularly for nucleotides, typically demands intricate organic synthesis techniques. In this study, we introduce a unique addition-elimination reaction method with a simple spin-labeling process, facilitating the monitoring of structural changes within nucleotide sequence. Our investigation focuses on three distinct labeling positions with a DNA sequence, allowing the measurement of distance between two spin-labels. The experimental mean distances obtained agreed with the calculated distances, underscoring the efficacy of this straightforward spin-labelling approach in studying complex biological processes such as transcription mechanism using EPR measurements.

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