Single-cell sequencing unveils the impact of aging on the progenitor cell diversity in the telencephalon of the female killifish N. furzeri.
aging
killifish
neurogenesis
progenitor cells
single‐cell sequencing
telencephalon
Journal
Aging cell
ISSN: 1474-9726
Titre abrégé: Aging Cell
Pays: England
ID NLM: 101130839
Informations de publication
Date de publication:
01 Jul 2024
01 Jul 2024
Historique:
revised:
04
06
2024
received:
28
02
2024
accepted:
06
06
2024
medline:
1
7
2024
pubmed:
1
7
2024
entrez:
1
7
2024
Statut:
aheadofprint
Résumé
The African turquoise killifish (Nothobranchius furzeri) combines a short lifespan with spontaneous age-associated loss of neuro-regenerative capacity, an intriguing trait atypical for a teleost. The impact of aging on the cellular composition of the adult stem cell niches, leading to this dramatic decline in the postnatal neuro- and gliogenesis, remains elusive. Single-cell RNA sequencing of the telencephalon of young adult female killifish of the short-lived GRZ-AD strain unveiled progenitors of glial and non-glial nature, different excitatory and inhibitory neuron subtypes, as well as non-neural cell types. Sub-clustering of the progenitors identified four radial glia (RG) cell types, two non-glial progenitor (NGP) and four intermediate (intercell) cell states. Two astroglia-like, one ependymal, and one neuroepithelial-like (NE) RG subtype were found at different locations in the forebrain in line with their role, while proliferative, active NGPs were spread throughout. Lineage inference pointed to NE-RG and NGPs as start and intercessor populations for glio- and neurogenesis. Upon aging, single-cell RNA sequencing revealed major perturbations in the proportions of the astroglia and intercell states, and in the molecular signatures of specific subtypes, including altered MAPK, mTOR, Notch, and Wnt pathways. This cell catalog of the young regeneration-competent killifish telencephalon, combined with the evidence for aging-related transcriptomic changes, presents a useful resource to understand the molecular basis of age-dependent neuroplasticity. This data is also available through an online database (killifishbrain_scseq).
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14251Subventions
Organisme : Onderzoeksraad, KU Leuven
ID : C3/21/012
Organisme : Onderzoeksraad, KU Leuven
ID : KA/16/020
Organisme : Onderzoeksraad, KU Leuven
ID : KA/20/013
Organisme : Onderzoeksraad, KU Leuven
ID : VTI-23-00197
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 1S00318N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G0C2618N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G0C9922N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : I013018N
Informations de copyright
© 2024 The Author(s). Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.
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