Phenotypic characterisation of bovine alveolar macrophages reveals two major subsets with differential expression of CD163.
Animals
Cattle
Macrophages, Alveolar
/ metabolism
Antigens, Differentiation, Myelomonocytic
/ metabolism
Antigens, CD
/ metabolism
Receptors, Cell Surface
/ metabolism
Phenotype
Mycobacterium bovis
/ immunology
Flow Cytometry
Tuberculosis, Bovine
/ metabolism
Immunophenotyping
Bronchoalveolar Lavage Fluid
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 06 2024
28 06 2024
Historique:
received:
12
03
2024
accepted:
25
06
2024
medline:
2
7
2024
pubmed:
2
7
2024
entrez:
1
7
2024
Statut:
epublish
Résumé
Bovine alveolar macrophages (AMs) defend the lungs against pathogens such as Mycobacterium bovis (M. bovis), the causative agent of bovine tuberculosis. However, little is known about the surface molecules expressed by bovine AMs and whether there is heterogeneity within the population. The purpose of this study was to characterise the bovine AM cell surface phenotype using flow cytometry. Bronchoalveolar lavage samples from four different calves were stained with a combination of antibodies against immune cell molecules prior to flow cytometric analysis. To assess the degree of expression, we considered the distribution and relative intensities of stained and unstained cells. We demonstrated that bovine AMs have high expression of CD172a, ADGRE1, CD206, and CD14, moderate expression of CD80, MHC II, CD1b, and CD40, low expression of CX3CR1 and CD86, and little or no expression of CD16 and CD26. Two distinct subsets of bovine AMs were identified based on CD163 expression. Subsequent analysis showed that the CD163
Identifiants
pubmed: 38951667
doi: 10.1038/s41598-024-65868-7
pii: 10.1038/s41598-024-65868-7
doi:
Substances chimiques
CD163 antigen
0
Antigens, Differentiation, Myelomonocytic
0
Antigens, CD
0
Receptors, Cell Surface
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
14974Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T00875X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/CCG2270/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/RL/230002B
Pays : United Kingdom
Informations de copyright
© 2024. The Author(s).
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