Oral Intake of Linseed Oil Inhibits Skin Barrier Dysfunction in Obese Mice.

Obesity is not only a risk factor for lifestyle-related diseases but also causes skin barrier dysfunction, which leads to a reduced quality of life due to dryness, itching, and scratching, and thus requires appropriate treatment. However, there are no studies on this issue. Therefore, this study aimed to examine whether oral intake of linseed oil is effective for skin barrier function in obesity and to confirm how the effect is demonstrated. TSOD mice received either sterile distilled water (Control group) or linseed oil (Omega group), containing a high level of omega-3 fatty acids, including α-linolenic acid, orally for eight weeks. Mice were then irradiated with ultraviolet B (UVB) and three days later, transepidermal water loss (TEWL), which is the primary outcome of skin barrier function, was measured and gross skin appearance was observed. Hematoxylin and eosin (HE) staining and Ki-67 immunostaining were performed on skin samples. mRNA expression levels of the inflammatory markers Compared with the Control group, the Omega group exhibited lower TEWL values and little skin erythema. Histological analysis revealed thinner epidermis and fewer Ki-67 positive cells. Additionally, in the Omega group, mRNA levels of four inflammation-related genes were lower, α-linolenic acid levels in both skin and erythrocytes were higher, and a lower n-6/n-3 ratio was observed. And α-linolenic acid levels in the skin were negatively correlated with the expression levels of inflammation-related genes. Oral intake of linseed oil was found to inhibit skin barrier dysfunction in obesity. This effect was mediated by α-linolenic acid, a major component of linseed oil with anti-inflammatory properties, which was taken up by erythrocytes and supplied to the skin. Therefore, oral intake of linseed oil is expected to be a useful therapeutic method for skin barrier dysfunction in obesity.

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Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Yokohama City University Animal Experiment Committee Issued protocol number F-A-23-051. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: This study was supported by the Japan Academy of Nursing Science (JANS) and the Association for Fordays Self-Reliance Support in Japan. Financial relationships: Yoshiko Horie declare(s) a grant from Japan Academy of Nursing Science (JANS) . Yoshiko Horie declare(s) a grant from Association for Fordays Self-Reliance Support in Japan. Toru Moriguchi (Chairman), Akiko Harauma (Director) declare(s) non-financial support from Japan Society for Lipid Nutrition. Toru Moriguchi, Akiko Harauma declare(s) a grant from Ota Oil Co., Ltd. Endowment course. Toru Moriguchi declare(s) non-financial support from Krill Association. Director. Toru Moriguchi declare(s) non-financial support from Society of Preventive Medicine for Animals and Humans. Director. Intellectual property info: Akiko Harauma and Toru Moriguchi have a patent planned, issued, or pending: 095P001JP1; Toru Moriguchi has a patent planned, issued, or pending: TPJ0508AZU. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.