Histological evaluation of different concentrations of hyaluronic-acid-added zinc oxide eugenol on rat molar pulp.
Dental pulp
Hyaluronic acid
In vitro/in vivo tests
Pulpotomy
Rat molars
Zinc oxide eugenol
Journal
Odontology
ISSN: 1618-1255
Titre abrégé: Odontology
Pays: Japan
ID NLM: 101134822
Informations de publication
Date de publication:
03 Jul 2024
03 Jul 2024
Historique:
received:
28
03
2024
accepted:
20
06
2024
medline:
3
7
2024
pubmed:
3
7
2024
entrez:
3
7
2024
Statut:
aheadofprint
Résumé
Hyaluronic acid (HA), known for diverse properties, was investigated for its potential in dental pulp therapy. This study investigated the potential of HA in dental pulp therapy by examining the physical properties and effects of zinc oxide eugenol (ZOE) pulpotomy materials containing varying HA concentrations on rat molar teeth. In vitro tests assessed compressive strength and hardness of ZOE materials blended with HA (0.5%, 1%, 3%) and HA gels (0.54%, 0.8%). 120 samples, encompassing the control group, underwent compressive strength testing, while 60 samples were designated for hardness assessment. In vivo experiments on rat molars studied histological effects of HA-containing ZOE on dental pulp over 1 week and 1 month. Gels with HA concentrations of 0.5%, 1%, and 0.54% were used in pulpotomy on 22 rats. Each rat underwent the procedure on four teeth, with one tooth serving as a control, totaling 88 teeth subjected to the intervention. In the analyses, SPSS 22.0 was used and the significance level was set at P = 0.05. Findings showed that HA at 0.5% maintained compressive strength, but higher concentrations decreased mechanical properties significantly (P = 0.001). Histological assessments indicated better outcomes with lower HA concentrations in terms of odontoblast layer continuity (P = 0.005 at 1 month) and pulp vitality (P = 0.001 at 1 week and P = 0.018 at 1 month). The study suggests HA holds promise for pulpotomy and regenerative endodontic treatments, but further research is needed to understand long-term clinical implications.
Identifiants
pubmed: 38958828
doi: 10.1007/s10266-024-00973-7
pii: 10.1007/s10266-024-00973-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Marmara Üniversitesi
ID : TDK-2022-10323
Informations de copyright
© 2024. The Author(s).
Références
Crystal YO, Fuks AB. Pulpotomy for primary teeth: techniques and materials. In: Fuks AB, Moskovitz M, Tickotsky N, editors. Contemporary endodontics for children and adolescents. Cham: Springer; 2023. p. 201–22.
doi: 10.1007/978-3-031-23980-9_13
Santos JM, Marques JA, Diogo P, Messias A, Sousa V, Sequeira D, et al. Influence of preoperative pulp inflammation in the outcome of full pulpotomy using a dog model. J Endod. 2021;47(9):1417–26. https://doi.org/10.1016/j.joen.2021.06.018 .
doi: 10.1016/j.joen.2021.06.018
pubmed: 34229033
Gonzalez-Lara A, Ruiz-Rodriguez MS, Pierdant-Perez M, Garrocho-Rangel JA, Pozos-Guillen AJ. Zinc oxide–eugenol pulpotomy in primary teeth: a 24 month follow-up. J Clin Pediatr Dent. 2016;40:107–12.
doi: 10.17796/1053-4628-40.2.107
pubmed: 26950810
Coll JA, Vargas K, Marghalani AA, Chen CY, AlShamali S, Dhar V, Crystal YO. A systematic review and meta-analysis of nonvital pulp therapy for primary teeth. Pediatr Dent. 2020;42:256–461.
pubmed: 32847665
Chircov C, Grumezescu AM, Bejenaru LE. Hyaluronic acid-based scaffolds for tissue engineering. Rom J Morphol Embryol. 2018;59:71–6.
pubmed: 29940614
Casale M, Moffa A, Vella P, Sabatino L, Capuano F, Salvinelli B, Lopez MA, Carinci F, Salvinelli F. Hyaluronic acid: perspectives in dentistry .A systematic review. Int J Immunopathol Pharmacol. 2016;29:572–82. https://doi.org/10.1177/0394632016652906 .
doi: 10.1177/0394632016652906
pubmed: 27280412
pmcid: 5806851
Selyanin M, Boykov P, Khabarov V, Polyak F. Chemical modifications, solid phase, radio-chemical and enzymatic transformations of hyaluronic acid. In: Hyaluronic acid: production, properties, application in biology and medicine. New Jersey: Wiley; 2015. p. 121–41.
doi: 10.1002/9781118695920.ch5
Sasaki T, Kawamata-Kido H. Providing an environment for reparative dentine induction in amputated rat molar pulp by high molecular-weight hyaluronic acid. Arch Oral Biol. 1995;40:209–19. https://doi.org/10.1016/0003-9969(95)98810-l .
doi: 10.1016/0003-9969(95)98810-l
pubmed: 7605248
Inuyama Y, Kitamura C, Nishihara T, Morotomi T, Nagayoshi M, Tabata Y, Matsuo K, Chen KK, Terashita M. Effects of hyaluronic acid sponge as a scaffold on odontoblastic cell line and amputated dental pulp. J Biomed Mater Res B Appl Biomater. 2010;92:120–8. https://doi.org/10.1002/jbm.b.31497 .
doi: 10.1002/jbm.b.31497
pubmed: 19802830
Ahmadian E, Eftekhari A, Dizaj SM, Sharifi S, Mokhtarpour M, Nasibova AN, Khalilov R, Samiei M. The effect of hyaluronic acid hydrogels on dental pulp stem cells behavior. Int J Biol Macromol. 2019;140:245–54. https://doi.org/10.1016/j.ijbiomac.2019.08.119 .
doi: 10.1016/j.ijbiomac.2019.08.119
pubmed: 31419560
Ildeş GÇ, Sezgin BI, Vieira AR, Mentes A. A randomized clinical trial of hyaluronic acid gel pulpotomy in primary molars with 1 year follow-up. Acta Odontol Scand. 2022;80:273–80. https://doi.org/10.1080/00016357.2021.1998612 .
doi: 10.1080/00016357.2021.1998612
pubmed: 34752723
Linde A. A study of the dental pulp glycosaminoglycans from permanent human teeth and rat and rabbit incisors. Arch Oral Biol. 1973;18:49–59. https://doi.org/10.1016/0003-9969(73)90019-8 .
doi: 10.1016/0003-9969(73)90019-8
pubmed: 4267480
Felszeghy S, Hyttinen M, Tammi R, Tammi M, Módis L. Quantitative image analysis of hyaluronan expression in human tooth germs. Eur J Oral Sci. 2000;108:320–6. https://doi.org/10.1034/j.1600-0722.2000.108004311.x .
doi: 10.1034/j.1600-0722.2000.108004311.x
pubmed: 10946767
Grewal N, Salhan R, Kaur N, Patel HB. Comparative evaluation of calcium silicate-based dentin substitute (Biodentine®) and calcium hydroxide (pulpdent) in the formation of reactive dentin bridge in regenerative pulpotomy of vital primary teeth: triple blind, randomized clinical trial. Contemp Clin Dent. 2016;7:457–63. https://doi.org/10.4103/0976-237x.194116 .
doi: 10.4103/0976-237x.194116
pubmed: 27994411
pmcid: 5141658
Kiranmayi T, Vemagiri CT, Rayala C, Chandrappa V, Bathula H, Challagulla A. In vivo comparison of bioceramic putty and mineral trioxide aggregate as pulpotomy medicament in primary molars. A 12 month follow-up randomized clinical trial. Dent Res J (Isfahan). 2022;19:84.
doi: 10.4103/1735-3327.359322
pubmed: 36426282
Galvão M, Caldas SGFR, Calabrez-Filho S, Campos EAD, Bagnato VS, Rastelli ANDS, Andrate MF. Compressive strength of dental composites photo-activated with different light tips. Laser Phys. 2013;23:045604. https://doi.org/10.1088/1054-660X/23/4/045604 .
doi: 10.1088/1054-660X/23/4/045604
Lee SY, Wang CC, Chen DC, Lai YL. Retentive and compressive strengths of modified zinc oxide-eugenol cements. J Dent. 2000;28:69–75.
doi: 10.1016/S0300-5712(99)00040-8
pubmed: 10666964
O’Brien WJ. Dental materials and their selection. 4th ed. Chicago: Quintessence; 2002. p. 12–25.
Shen C, Rawls HR, Esquivel-Upshaw JF. Mechanical properties of solids. In: Phillips’ science of dental materials e-book. 13th ed. St. Louis: Elsevier Health Sciences; 2021. p. 64–87.
Cengiz SB, Batirbaygil Y, Onur MA, Atilla P, Asan E, Altay N, Cehreli ZC. Histological comparison of alendronate, calcium hydroxide and formocresol in amputated rat molar. Dent Traumatol. 2005;21:281–8. https://doi.org/10.1111/j.1600-9657.2005.00325.x .
doi: 10.1111/j.1600-9657.2005.00325.x
pubmed: 16149924
Ajay Sharma L, Love RM, Ali MA, Sharma A, Macari S, Avadhani A, Dias GJ. Healing response of rat pulp treated with an injectable keratin hydrogel. J Appl Biomater Funct Mater. 2017;15:244–50. https://doi.org/10.5301/jabfm.5000346 .
doi: 10.5301/jabfm.5000346
pmcid: 6379890
Lopes CS, Junqueira MA, Cosme-Silva L, Pegoraro COR, Garbelini CCD, Oliveira TM, Martins NS, Neves JDS, Sakai VT. Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate. J Appl Oral Sci. 2019;27:e20180550. https://doi.org/10.1590/1678-7757-2018-0550 .
doi: 10.1590/1678-7757-2018-0550
pubmed: 31365709
pmcid: 6690728
Chen KL, Yeh YY, Lung J, Yang YC, Yuan K. Mineralization effect of hyaluronan on dental pulp cells via CD44. J Endod. 2016;42:711–6. https://doi.org/10.1016/j.joen.2016.01.010 .
doi: 10.1016/j.joen.2016.01.010
pubmed: 26975415
Silva CR, Babo PS, Gulino M, Costa L, Oliveira JM, Silva-Correia J, Domingues RMA, Reis RL, Gomes ME. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration. Acta Biomater. 2018;77:155–71. https://doi.org/10.1016/j.actbio.2018.07.035 .
doi: 10.1016/j.actbio.2018.07.035
pubmed: 30031163
Borzacchiello A, Russo L, Zaccaria S, Ambrosio L. Physical and chemical hyaluronic acid hydrogels and their biomedical applications. Polysacch Hydrogels Charact Biomed Appl. 2016. https://doi.org/10.1201/b19751-13 .
doi: 10.1201/b19751-13
Manappallil JJ. Basic dental materials. 4th ed. New Delhi: JP Medical Ltd; 2015. p. 84–128.
Anderson JR Jr, Myers GE. Physical properties of some zinc oxide-eugenol cements. J Dent Res. 1966;45:379–87. https://doi.org/10.1177/00220345660450022801 .
doi: 10.1177/00220345660450022801
pubmed: 5220625
Soltan Karimy V, Bayanzadeh S. Evaluation of the physical properties of the Iranian temporary cement, Tem Band, and the foreign one, Temp Bond, compared to ISO 3107. J Dent Med. 2006;19:84–90.
Bakhori SKM, Mahmud S, Mohamad D, Masudi SM, Seeni A. Surface morphological and mechanical properties of zinc oxide eugenol using different types of ZnO nanopowder. Mater Sci Eng C Mater Biol Appl. 2019;100:645–54. https://doi.org/10.1016/j.msec.2019.03.034 .
doi: 10.1016/j.msec.2019.03.034
pubmed: 30948101
Tanaka Y, Gong JP, Osada Y. Novel hydrogels with excellent mechanical performance. Prog Polym Sci. 2005;30:1–9. https://doi.org/10.1016/j.progpolymsci.2004.11.003 .
doi: 10.1016/j.progpolymsci.2004.11.003
Bogović A, Nižetić J, Galić N, Želježić D, Micek V, Mladinić M. The effects of hyaluronic acid, calcium hydroxide, and dentin adhesive on rat odontoblasts and fibroblasts. Arh Hig Rada Toksikol. 2011;62:155–61. https://doi.org/10.2478/10004-1254-62-2011-2076 .
doi: 10.2478/10004-1254-62-2011-2076
pubmed: 21705303
Umemura N, Ohkoshi E, Tajima M, Kikuchi H, Katayama T, Sakagami H. Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44. Stem Cell Res Ther. 2016;7:1–12. https://doi.org/10.1186/s13287-016-0399-8 .
doi: 10.1186/s13287-016-0399-8
Chrepa V, Austah O, Diogenes A. Evaluation of a commercially available hyaluronic acid hydrogel (Restylane) as injectable scaffold for dental pulp regeneration: an in vitro evaluation. J Endod. 2017;43:257–62. https://doi.org/10.1016/j.joen.2016.10.026 .
doi: 10.1016/j.joen.2016.10.026
pubmed: 28041686
Mahfouz SM, Abdallah A, El-desouky SS. Clinical and radiographic evaluation of hyaluronic acid as a vital pulpotomy medication in primary molars. Egypt Dent J. 2021;67:1843–55.
doi: 10.21608/edj.2021.74238.1611
Palma PJ, Ramos JC, Martins JB, Diogenes A, Figueiredo MH, Ferreira P, et al. Histologic evaluation of regenerative endodontic procedures with the use of chitosan scaffolds in immature dog teeth with apical periodontitis. J Endod. 2017;43(8):1279–87. https://doi.org/10.1016/j.joen.2017.03.005 .
doi: 10.1016/j.joen.2017.03.005
pubmed: 28577961
d’Souza R, Bachman T, Baumgardner K, Butler W, Litz M. Characterization of cellular responses involved in reparative dentinogenesis in rat molars. J Dent Res. 1995;74:702–9. https://doi.org/10.1177/00220345950740021301 .
doi: 10.1177/00220345950740021301
pubmed: 7722069
Dammaschke T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Lab Anim. 2010;44:1–6. https://doi.org/10.1258/la.2009.008120 .
doi: 10.1258/la.2009.008120
pubmed: 19854755
Berman DS, Massler M. Experimental pulpotomies in rat molars. J Dent Res. 1958;37(2):229–42. https://doi.org/10.1177/00220345580370020701 .
doi: 10.1177/00220345580370020701
pubmed: 13525508
Goldberg M, Six N, Decup F, Buch D, Soheili Majd E, Lasfargues J-J, et al. Application of bioactive molecules in pulp-capping situations. Adv Dent Res. 2001;15(1):91–5. https://doi.org/10.1177/08959374010150012401 .
doi: 10.1177/08959374010150012401
pubmed: 12640750
Palma PJ, Marques JA, Falacho RI, Vinagre A, Santos JM, Ramos JC. Does delayed restoration improve shear bond strength of different restorative protocols to calcium silicate-based cements? Materials. 2018;11(11):2216. https://doi.org/10.3390/ma11112216 .
doi: 10.3390/ma11112216
pubmed: 30413054
pmcid: 6265959