The root-knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism.
Meloidogyne incognita
Nicotiana benthamiana
Solanum lycopersicum
ER quality control
effector
endoplasmic reticulum (ER)
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
received:
07
06
2024
accepted:
17
06
2024
medline:
4
7
2024
pubmed:
4
7
2024
entrez:
4
7
2024
Statut:
ppublish
Résumé
Root-knot nematodes (RKNs) are microscopic parasitic worms able to infest the roots of thousands of plant species, causing massive crop yield losses worldwide. They evade the plant's immune system and manipulate plant cell physiology and metabolism to transform a few root cells into giant cells, which serve as feeding sites for the nematode. RKN parasitism is facilitated by the secretion in planta of effector molecules, mostly proteins that hijack host cellular processes. We describe here a conserved RKN-specific effector, effector 12 (EFF12), that is synthesized exclusively in the oesophageal glands of the nematode, and we demonstrate its function in parasitism. In the plant, MiEFF12 localizes to the endoplasmic reticulum (ER). A combination of RNA-sequencing analysis and immunity-suppression bioassays revealed the contribution of MiEFF12 to the modulation of host immunity. Yeast two-hybrid, split luciferase and co-immunoprecipitation approaches identified an essential component of the ER quality control system, the Solanum lycopersicum plant bap-like (PBL), and basic leucine zipper 60 (BZIP60) proteins as host targets of MiEFF12. Finally, silencing the PBL genes in Nicotiana benthamiana decreased susceptibility to Meloidogyne incognita infection. Our results suggest that EFF12 manipulates PBL function to modify plant immune responses to allow parasitism.
Substances chimiques
Helminth Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13491Subventions
Organisme : IDEX UCAJedi
ID : ANR-15-IDEX-0001
Organisme : ANR MASH
ID : ANR-21-CE20-0002
Organisme : LabEx Saclay Plant Sciences
ID : ANR-10-LABX-40
Organisme : National Key Research and Development Program of China
ID : 2023YFD1400400
Organisme : LabEx Signalife
ID : ANR-11-LABX-0028-01
Informations de copyright
© 2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
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