Effects of low-intensity pulsed ultrasound on the microorganisms of expressed prostatic secretion in patients with IIIB prostatitis.
Expressed prostatic secretion
High throughput
IIIB prostatitis
Low-intensity pulsed ultrasound
Microorganism
Second-generation sequencing
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 Jul 2024
04 Jul 2024
Historique:
received:
06
12
2023
accepted:
01
07
2024
medline:
5
7
2024
pubmed:
5
7
2024
entrez:
4
7
2024
Statut:
epublish
Résumé
To detect and analyze the changes of microorganisms in expressed prostatic secretion (EPS) of patients with IIIB prostatitis before and after low-intensity pulsed ultrasound (LIPUS) treatment, and to explore the mechanism of LIPUS in the treatment of chronic prostatitis (CP). 25 patients (study power was estimated using a Dirichlet-multinomial approach and reached 96.5% at α = 0.05 using a sample size of 25) with IIIB prostatitis who were effective in LIPUS treatment were divided into two groups before and after LIPUS treatment. High throughput second-generation sequencing technique was used to detect and analyze the relative abundance of bacterial 16 s ribosomal variable regions in EPS before and after treatment. The data were analyzed by bioinformatics software and database, and differences with P < 0.05 were considered statistically significant. Beta diversity analysis showed that there was a significant difference between groups (P = 0.046). LEfSe detected four kinds of characteristic microorganisms in the EPS of patients with IIIB prostatitis before and after LIPUS treatment. After multiple comparisons among groups by DESeq2 method, six different microorganisms were found. LIPUS may improve patients' clinical symptoms by changing the flora structure of EPS, stabilizing and affecting resident bacteria or opportunistic pathogens.
Identifiants
pubmed: 38965410
doi: 10.1038/s41598-024-66329-x
pii: 10.1038/s41598-024-66329-x
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15368Subventions
Organisme : National Natural Science Foundation of China
ID : 82101694
Informations de copyright
© 2024. The Author(s).
Références
Wang, Y. B., He, D. H. & Yang, H. T. Clinical observation of low energy extracorporeal shock wave in the treatment of type IIIB prostatitis. Chin. Community Phys. 34(22), 77–79 (2018).
Song, W. J., Liu, X. H. & He, L. Y. Research progress on the relationship between prostate disease and erectile dysfunction. J. Urol. 11(04), 35–38 (2019).
Banyra, O., Ivanenko, O., Nikitin, O. & Shulyak, A. Mental status in patients with chronic bacterial prostatitis. Cent. Eur. J. Urol. 66(1), 93–100 (2013).
doi: 10.5173/ceju.2013.01.art29
Yu, X. J. & Gao, Q. H. Guidelines for multidisciplinary diagnosis and treatment of chronic prostatitis with integrated traditional Chinese and western medicine. Chin. J. Androl. 26(04), 82–89 (2020).
Mo, X. et al. Prevalence and correlates of Mycoplasma genitalium infection among prostatitis patients in Shanghai, China. Sex. Health 13, 474–479 (2016).
doi: 10.1071/SH15155
Xiao, J. Q. et al. Study on pathogenic microorganisms of prostatic fluid in patients with refractory chronic prostatitis. Chin. J. Androl. 10, 5 (2010).
Wu, Y. et al. Screening for chronic prostatitis pathogens using high-throughput next-generation sequencing. Prostate 80, 577–587 (2020).
doi: 10.1002/pros.23971
pubmed: 32162709
pmcid: 7187444
Franco, J. V. et al. Non-pharmacological interventions for treating chronic prostatitis/chronic pelvic pain syndrome. Cochrane Database Syst. Rev. 1, CD012551 (2018).
pubmed: 29372565
Franco, J. V. et al. Pharmacological interventions for treating chronic prostatitis/chronic pelvic pain syndrome. Cochrane Database Syst. Rev. 10, CD012552 (2019).
pubmed: 31587256
Yuan, P. et al. Efficacy of low-intensity extracorporeal shock wave therapy for the treatment of chronic prostatitis/chronic pelvic pain syndrome: A systematic review and meta-analysis. Neurourol. Urodyn. 38, 1457–1466 (2019).
doi: 10.1002/nau.24017
pubmed: 31037757
Zhu, X. D. et al. Clinical analysis of low energy shock wave in the treatment of intractable type III B prostatitis. J. Clin. Urol. 10, 4 (2020).
Mykoniatis, I. et al. Evaluation of a low-intensity shockwave therapy for chronic prostatitis type IIIb/chronic pelvic pain syndrome: A double-blind randomized sham-controlled clinical trial. Prostate Cancer Prostatic Dis. 24, 370–379 (2021).
doi: 10.1038/s41391-020-00284-2
pubmed: 32989261
La, R. P. S. et al. Hypothesis testing and power calculations for taxonomic-based human microbiome data. PLoS One 7(12), e52078 (2012).
Callahan, B. J. et al. DADA2: High-resolution sample inference from Illumina amplicon data. Nat. Methods. 13(7), 581–583. https://doi.org/10.1038/nmeth.3869 (2016). PMID: 27214047; PMCID: PMC4927377.
Bokulich, N. A. et al. Optimizing taxonomic classification of marker gene amplicon sequences. Microbiome https://doi.org/10.1186/s40168-018-0470-z (2018).
doi: 10.1186/s40168-018-0470-z
pubmed: 29773078
pmcid: 5956843
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15(12), 550 (2014).
doi: 10.1186/s13059-014-0550-8
pubmed: 25516281
pmcid: 4302049
Vázquez-Baeza, Y. et al. EMPeror: A tool for visualizing high-throughput microbial community data. Gigascience 2, 2047 (2019).
Rohart, F. et al. mixOmics: An R package for ‘omics feature selection and multiple data integration. PLoS Comput. Biol. 13(11), e1005752 (2017).
doi: 10.1371/journal.pcbi.1005752
pubmed: 29099853
pmcid: 5687754
Mandal, S. et al. Analysis of composition of microbiomes: A novel method for studying microbial composition. Microb. Ecol. Health Dis. 26, 27663 (2015).
pubmed: 26028277
DeSantis, T. Z. et al. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl. Environ. Microbiol. 72, 5069–5072 (2006).
doi: 10.1128/AEM.03006-05
pubmed: 16820507
pmcid: 1489311
Videcnik, Z. J. et al. Diagnosis and treatment of bacterial prostatitis. Acta Dermatovenerol. Alp Pannonica Adriat. 24(2), 25–29 (2015).
Nickel, J. C. Prostatitis. Can. Urol. Assoc. J. 5(5), 306–315 (2011).
doi: 10.5489/cuaj.686
pubmed: 22031609
pmcid: 3202001
Arora, H. C., Eng, C. & Shoskes, D. A. Gut microbiome and chronic prostatitis/chronic pelvic pain syndrome. Ann. Transl. Med. 5(2), 30 (2017).
doi: 10.21037/atm.2016.12.32
pubmed: 28217695
pmcid: 5300850
Skerk, V. et al. Chronic prostatitis caused by Trichomonas vaginalis—Diagnosis and treatment. J. Chemother. 14(5), 537–538 (2002).
doi: 10.1179/joc.2002.14.5.537
pubmed: 12462437
Irajian, G. et al. Molecular detectionof Ureaplasma urealyticum from prostate tissues using PCR-RFLP, Tehran, Iran. Iran. J. Pathol. 11(2), 138–143 (2016).
pubmed: 27499775
pmcid: 4939644
Zimmermann, R., Cumpanas, A., Miclea, F. & Janetschek, G. Extracorporeal shock wave therapy for the treatment of chronic pelvic pain syndrome in males: A randomised, double-blind, placebocontrolled study. Eur. Urol. 56, 418–424 (2009).
doi: 10.1016/j.eururo.2009.03.043
pubmed: 19372000
Marszalek, M., Berger, I. & Madersbacher, S. Low-energy extracorporeal shock wave therapy for chronic pelvic pain syndrome: Finally, the magic bullet?. Eur. Urol. 56, 425–426 (2009).
doi: 10.1016/j.eururo.2009.03.075
pubmed: 19362412
Cavarretta, I. et al. The microbiome of the prostate tumor microenvironment. Eur Urol 72(4), 625–631 (2017).
doi: 10.1016/j.eururo.2017.03.029
pubmed: 28434677
Fang, D. B. Study on the Diversity of Microflora in Semen and Prostate Massage Fluid of Patients With Type III Prostatitis (Zhejiang University, 2014).
Alfano, M. et al. Testicular microbiomein azoospermic men-first evidence of the impact of an altered microenvironment. Hum. Reprod. 33(7), 1212–1217 (2018).
doi: 10.1093/humrep/dey116
pubmed: 29850857
pmcid: 6012977
Li, Y., Zhou, Y. C. & Shang, X. J. Effects of reproductive system microflora on male health and related diseases. J. Clin. Urol. 27(11), 1030–1034 (2021).
Davidsson, S. et al. Frequency and typing of Propionibacterium acnes in prostate tissue obtained from men with and without prostate cancer. Infect. Agents Cancer 11, 26 (2016).
doi: 10.1186/s13027-016-0074-9
Feng, Y. et al. Metagenomic and metatranscriptomic analysis of human prostate microbiota from patients with prostate cancer. BMC Genom. 20(1), 146 (2019).
doi: 10.1186/s12864-019-5457-z
Lee, G. Chronic prostatitis: A possible cause of hematospermia. World J Mens Health 33(2), 103–108 (2015).
doi: 10.5534/wjmh.2015.33.2.103
pubmed: 26331127
pmcid: 4550592
Wood, R. W. & Loomis, A. L. The physical and biological effects of high-frequency sound-waves of great intensity. Lond. Edinb. Dublin Philos. Mag. J. Sci. 4, 417–436 (1927).
doi: 10.1080/14786440908564348
Zhang, Y. I. et al. Hematoporphyrin monomethyl ether mediated sonodynamic antimicrobial chemotherapy on porphyromonas gingivalis in vitro. Microb. Pathog. 144, 104192 (2020).
doi: 10.1016/j.micpath.2020.104192
pubmed: 32272214
Bhavya, M. L. & Hebbar, H. U. Sono-photodynamic inactivation of Escherichia coli and Staphylococcus aureus in orange juice. Ultrason. Sonochem. 57, 108–115 (2019).
doi: 10.1016/j.ultsonch.2019.05.002
pubmed: 31208605
Fan, L. et al. Sonodynamic antimicrobial chemotherapy: An emerging alternative strategy for microbial inactivation. Ultrason. Sonochem. 75(3), 105591 (2021).
doi: 10.1016/j.ultsonch.2021.105591
pubmed: 34082219
pmcid: 8182071