Prospective, International, Multisite Comparison of Platelet Isolation Techniques for Genome-Wide Transcriptomics: Communication from the SSC of the ISTH.

Platelets leukocytes next generation RNA Seq platelet transcriptomics

Journal

Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836
Titre abrégé: J Thromb Haemost
Pays: England
ID NLM: 101170508

Informations de publication

Date de publication:
03 Jul 2024
Historique:
received: 09 05 2024
revised: 17 06 2024
accepted: 19 06 2024
medline: 6 7 2024
pubmed: 6 7 2024
entrez: 5 7 2024
Statut: aheadofprint

Résumé

Genome-wide platelet transcriptomics is increasingly used to uncover new aspects of platelet biology and as a diagnostic and prognostic tool. Nevertheless, platelet isolation methods for transcriptomic studies are not standardized, introducing challenges for cross-study comparisons, data integration, and replication. In this prospective multicenter study, called "Standardizing Platelet Transcriptomics for Discovery, Diagnostics, and Therapeutics in the Thrombosis and Hemostasis Community (STRIDE)" by the ISTH SSCs, we assessed how three of the most commonly used platelet isolation protocols influence metrics from next-generation bulk RNA sequencing and functional assays. Compared with washing alone, more stringent removal of leukocytes by anti-CD45 beads or PALL

Identifiants

DOI: 10.1016/j.jtha.2024.06.017 PMID: 38969303
pubmed: 38969303
pii: S1538-7836(24)00376-3
doi: 10.1016/j.jtha.2024.06.017
pii:
doi:

Types de publication

Journal Article Practice Guideline

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Auteurs

Meenakshi Banerjee (M)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA.

Jesse W Rowley (JW)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA; Department of Internal Medicine, University of Utah Health, Salt Lake City, Utah, USA.

Chris J Stubben (CJ)

Bioinformatics Shared Resource, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA.

Neal D Tolley (ND)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA.

Kathleen Freson (K)

Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, KULeuven, Leuven, Belgium.

Benjamin Nelson (B)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA.

Béla Nagy (B)

Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Zsolt Fejes (Z)

Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Antoinette M Blair (AM)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA.

Ernest Turro (E)

Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Paolo Gresele (P)

Department of Medicine and Surgery, University of Perugia, Italy.

Giulia Ciarrocca Taranta (GC)

Department of Medicine and Surgery, University of Perugia, Italy.

Loredana Bury (L)

Department of Medicine and Surgery, University of Perugia, Italy.

Emanuela Falcinelli (E)

Department of Medicine and Surgery, University of Perugia, Italy.

Marie Lordkipanidzé (M)

Research Center, Montreal Heart Institute, Montreal, Quebec, Canada, H1T 1C8; Faculty of Pharmacy, Université de Montréal, Montreal, Quebec, Canada, H3T 1J4.

Marie-Christine Alessi (MC)

Cardiovascular and Nutrition Centre, C2VN, Aix Marseille Univ, INSERM, INRAE, Marseille, France.

Andrew D Johnson (AD)

Population Sci9ences Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, Framingham, Massachusetts, USA; The Framingham Heart Study, Framingham, Massachusetts, USA.

Tamam Bakchoul (T)

Transfusion Medicine, Medical Faculty of Tubingen, University of Tubingen, Tubingen, Germany.

Sofia Ramstrom (S)

Cardiovascular Research Centre, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Mattia Frontini (M)

Department of Clinical and Biomedical Sciences, University of Exeter Medical School, Faculty of Health and Life Sciences, RILD Building, Barrack Road, Exeter, EX2 5DW.

Marina Camera (M)

Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS, Milan, Italy; Department of Pharmaceutical Sciences, Università Degli Studi Di Milano, Milan, Italy.

Marta Brambilla (M)

Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS, Milan, Italy.

Robert A Campbell (RA)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA; Department of Internal Medicine, University of Utah Health, Salt Lake City, Utah, USA.

Matthew T Rondina (MT)

University of Utah Molecular Medicine Program, Eccles Institute of Human Genetics, Salt Lake City, Utah, USA; Department of Internal Medicine, University of Utah Health, Salt Lake City, Utah, USA; George E. Wahlen Veterans Affairs Medical Center & GRECC, Salt Lake City, Utah, USA.

Classifications MeSH