Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

Journal

Nature methods

ISSN: 1548-7105

Titre abrégé: Nat Methods

Pays: United States

ID NLM: 101215604

Informations de publication

Date de publication:
05 Jul 2024

Historique:

received: 20 11 2023

accepted: 10 06 2024

medline: 6 7 2024

pubmed: 6 7 2024

entrez: 5 7 2024

Statut: aheadofprint

Résumé

Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.

Identifiants

DOI: 10.1038/s41592-024-02344-0 PMID: 38969722

pubmed: 38969722

doi: 10.1038/s41592-024-02344-0

pii: 10.1038/s41592-024-02344-0

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : NSF | Directorate for Biological Sciences (BIO)

ID : 1943448

Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)

ID : 101004728

Informations de copyright

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