Breast Tumor Cell Survival and Morphology in a Brain-like Extracellular Matrix Depends on Matrix Composition and Mechanical Properties.

Matrigel PCL scaffolds breast tumor cells co‐cultures hyaluronic acid

Journal

Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319

Informations de publication

Date de publication:
06 Jul 2024
Historique:
revised: 22 05 2024
received: 15 04 2024
medline: 7 7 2024
pubmed: 7 7 2024
entrez: 7 7 2024
Statut: aheadofprint

Résumé

Triple-negative breast cancer (TNBC) is the most invasive type of breast cancer with high risk of brain metastasis. To better understand interactions between breast tumors with the brain extracellular matrix (ECM), a 3D cell culture model is implemented using a thiolated hyaluronic acid (HA-SH) based hydrogel. The latter is used as HA represents a major component of brain ECM. Melt-electrowritten (MEW) scaffolds of box- and triangular-shaped polycaprolactone (PCL) micro-fibers for hydrogel reinforcement are utilized. Two different molecular weight HA-SH materials (230 and 420 kDa) are used with elastic moduli of 148 ± 34 Pa (soft) and 1274 ± 440 Pa (stiff). Both hydrogels demonstrate similar porosities. The different molecular weight of HA-SH, however, significantly changes mechanical properties, e.g., stiffness, nonlinearity, and hysteresis. The breast tumor cell line MDA-MB-231 forms mainly multicellular aggregates in both HA-SH hydrogels but sustains high viability (75%). Supplementation of HA-SH hydrogels with ECM components does not affect gene expression but improves cell viability and impacts cellular distribution and morphology. The presence of other brain cell types further support numerous cell-cell interactions with tumor cells. In summary, the present 3D cell culture model represents a novel tool establishing a disease cell culture model in a systematic way.

Identifiants

pubmed: 38971965
doi: 10.1002/adbi.202400184
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2400184

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : 326998133

Informations de copyright

© 2024 The Author(s). Advanced Biology published by Wiley‐VCH GmbH.

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Auteurs

Esra Türker (E)

Institute for Clinical Neurobiology, University Hospital Würzburg, Versbacherstr. 5, 97078, Würzburg, Germany.

Mateo S Andrade Mier (MS)

Institute for Clinical Neurobiology, University Hospital Würzburg, Versbacherstr. 5, 97078, Würzburg, Germany.

Jessica Faber (J)

Institute of Continuum Mechanics and Biomechanics, FAU Erlangen-Nürnberg, Egerlandstr. 5, 91058, Erlangen, Germany.

Selma J Padilla Padilla (SJ)

Department of Biomaterials, Engineering Faculty, University of Bayreuth, Prof.-Rüdiger-Bormann-Straße 1, 95447, Bayreuth, Germany.

Nicoletta Murenu (N)

Institute for Clinical Neurobiology, University Hospital Würzburg, Versbacherstr. 5, 97078, Würzburg, Germany.

Philipp Stahlhut (P)

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

Gregor Lang (G)

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

Zan Lamberger (Z)

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

Jeanette Weigelt (J)

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

Natascha Schaefer (N)

Institute for Clinical Neurobiology, University Hospital Würzburg, Versbacherstr. 5, 97078, Würzburg, Germany.

Jörg Tessmar (J)

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

Pamela L Strissel (PL)

Institute of Pathology, Krankenhausstrasse 8-10, 91054, Erlangen, Germany.
Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD, USA.
University Hospital Erlangen, Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, FAU Erlangen-Nürnberg, Universitätsstr. 21/23, 91054, Erlangen, Germany.

Torsten Blunk (T)

Department of Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital Würzburg, Oberdürrbacherstr. 6, 97080, Würzburg, Germany.

Silvia Budday (S)

Institute of Continuum Mechanics and Biomechanics, FAU Erlangen-Nürnberg, Egerlandstr. 5, 91058, Erlangen, Germany.

Reiner Strick (R)

University Hospital Erlangen, Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, FAU Erlangen-Nürnberg, Universitätsstr. 21/23, 91054, Erlangen, Germany.

Carmen Villmann (C)

Institute for Clinical Neurobiology, University Hospital Würzburg, Versbacherstr. 5, 97078, Würzburg, Germany.

Classifications MeSH