Simultaneous genetic transformation and genome editing of mixed lines in soybean (

Robust genome editing technologies are becoming part of the crop breeding toolbox. Currently, genome editing is usually conducted either at a single locus, or multiple loci, in a variety at one time. Massively parallel genomics platforms, multifaceted genome editing capabilities, and flexible transformation systems enable targeted variation at nearly any locus, across the spectrum of genotypes within a species. We demonstrate here the simultaneous transformation and editing of many genotypes, by targeting mixed seed embryo explants with genome editing machinery, followed by re-identification through genotyping after plant regeneration. Transformation and Editing of Mixed Lines (TREDMIL) produced transformed individuals representing 101 of 104 (97%) mixed elite genotypes in soybean; and 22 of 40 (55%) and 9 of 36 (25%) mixed maize female and male elite inbred genotypes, respectively. Characterization of edited genotypes for the regenerated individuals identified over 800 distinct edits at the Determinate1 ( The online version contains supplementary material available at 10.1007/s42994-024-00173-5.

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© The Author(s) 2024.

Déclaration de conflit d'intérêts

Conflict of interestYC, BBT, DB, EC, JR, MV, TR, MH, SB and FA are current employees of Bayer CropScience, and YC, BBT, DB, EC, JR, MV, TR, MH, SB and FA were former employees of Monsanto Company, which was subsequently acquired by Bayer AG. Bayer CropScience develops and manufactures plant seeds and agricultural traits produced by conventional and biotechnology methods. Patent applications have been filed related to the subject matter of this manuscript and assigned to Monsanto or Bayer.