Extracellular vesicle isolation methods identify distinct HIV-1 particles released from chronically infected T-cells.
HIV‐1
NRTIs
amphisomes
cART
exomeres
exosomes
extracellular particles
extracellular vesicles
smHIV‐1
small HIV
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
received:
20
09
2023
accepted:
16
06
2024
medline:
9
7
2024
pubmed:
9
7
2024
entrez:
9
7
2024
Statut:
ppublish
Résumé
The current study analyzed the intersecting biophysical, biochemical, and functional properties of extracellular particles (EPs) with the human immunodeficiency virus type-1 (HIV-1) beyond the currently accepted size range for HIV-1. We isolated five fractions (Frac-A through Frac-E) from HIV-infected cells by sequential differential ultracentrifugation (DUC). All fractions showed a heterogeneous size distribution with median particle sizes greater than 100 nm for Frac-A through Frac-D but not for Frac-E, which contained small EPs with an average size well below 50 nm. Synchronized and released cultures contained large infectious EPs in Frac-A, with markers of amphisomes and viral components. Additionally, Frac-E uniquely contained EPs positive for CD63, HSP70, and HIV-1 proteins. Despite its small average size, Frac-E contained membrane-protected viral integrase, detectable only after SDS treatment, indicating that it is enclosed in vesicles. Single particle analysis with dSTORM further supported these findings as CD63, HIV-1 integrase, and the viral surface envelope (Env) glycoprotein (gp) colocalized on the same Frac-E particles. Surprisingly, Frac-E EPs were infectious, and infectivity was significantly reduced by immunodepleting Frac-E with anti-CD63, indicating the presence of this protein on the surface of infectious small EPs in Frac-E. To our knowledge, this is the first time that extracellular vesicle (EV) isolation methods have identified infectious small HIV-1 particles (
Substances chimiques
Tetraspanin 30
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12476Subventions
Organisme : NIH HHS
ID : AI043894
Pays : United States
Organisme : NIH HHS
ID : AI074410
Pays : United States
Organisme : NIH HHS
ID : AI078859
Pays : United States
Organisme : NIH HHS
ID : AI127351-01
Pays : United States
Organisme : NIH HHS
ID : MH134389
Pays : United States
Organisme : NIH HHS
ID : NS099029
Pays : United States
Organisme : Henry M. Jackson Foundation
ID : W81XWH-11-2-0174
Informations de copyright
© 2024 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
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