Alternative cryoprotective agent for corneal stroma-derived mesenchymal stromal cells for clinical applications.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 Jul 2024
09 Jul 2024
Historique:
received:
22
12
2023
accepted:
20
06
2024
medline:
10
7
2024
pubmed:
10
7
2024
entrez:
9
7
2024
Statut:
epublish
Résumé
Cryopreservation of human corneal stroma-derived mesenchymal stromal cells (hCS-MSCs) with dimethylsulfoxide (DMSO) as a cryoprotective agent (CPA) has not been previously compared to that with glycerol under standard conditions. The hCS-MSCs were hereby cryopreserved with both compounds using a freezing rate of 1 °C/minute. The CPAs were tested by different concentrations in complete Minimum Essential Medium (MEM) approved for good manufacturing practice, and a medium frequently used in cell laboratory culturing-Dulbecco's modified eagle serum. The hCS-MSCs were isolated from cadaveric human corneas obtained from the Norwegian Eye Bank, and immunophenotypically characterized by flow cytometry before and after cryopreservation. The survival rate, the cellular adhesion, proliferation and cell surface coverage after cryopreservation of hCS-MSCs has been studied. The hCS-MSCs were immunofluorescent stained and examined for their morphology microscopically. The results showed that cryopreservation of hCS-MSCs in MEM with 10% glycerol gives a higher proliferation rate compared to other cryopreserving media tested. Based on the results, hCS-MSCs can safely be cryopreserved using glycerol instead of the traditional use of DMSO.
Identifiants
pubmed: 38982099
doi: 10.1038/s41598-024-65469-4
pii: 10.1038/s41598-024-65469-4
doi:
Substances chimiques
Cryoprotective Agents
0
Glycerol
PDC6A3C0OX
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15788Subventions
Organisme : Norwegian Research Council
ID : E667269
Organisme : European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement
ID : 801133
Informations de copyright
© 2024. The Author(s).
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