Quantitative urinary proteome analysis reveals potential biomarkers for disease activity of Behcet's disease uveitis.
Behcet’s disease uveitis
Biomarkers
Data-independent acquisition
Tandem mass tags
Urine proteome
Journal
BMC ophthalmology
ISSN: 1471-2415
Titre abrégé: BMC Ophthalmol
Pays: England
ID NLM: 100967802
Informations de publication
Date de publication:
09 Jul 2024
09 Jul 2024
Historique:
received:
30
07
2023
accepted:
03
07
2024
medline:
10
7
2024
pubmed:
10
7
2024
entrez:
9
7
2024
Statut:
epublish
Résumé
Behçet's disease-associated uveitis (BDU) is a severe, recurrent inflammatory condition affecting the eye and is part of a systemic vasculitis with unknown etiology, making biomarker discovery essential for disease management. In this study, we intend to investigate potential urinary biomarkers to monitor the disease activity of BDU. Firstly, label-free data-dependent acquisition (DDA) and tandem mass tag (TMT)-labeled quantitative proteomics methods were used to profile the proteomes of urine from active and quiescent BDU patients, respectively. For further exploration, the remaining fifty urine samples were analyzed by a data-independent acquisition (DIA) quantitative proteomics method. Twenty-nine and 21 differential proteins were identified in the same urine from BDU patients by label-free DDA and TMT-labeled analyses, respectively. Seventy-nine differentially expressed proteins (DEPs) were significantly changed in other active BDU urine samples compared to those in quiescent BDU urine samples by IDA analysis. Gene Ontology (GO) and protein-protein interaction (PPI) analyses revealed that the DEPs were associated with multiple functions, including the immune and neutrophil activation responses. Finally, seven proteins were identified as candidate biomarkers for BDU monitoring and recurrence prediction, namely, CD38, KCRB, DPP4, FUCA2, MTPN, S100A8 and S100A9. Our results showed that urine can be a good source of biomarkers for BDU. These dysregulated proteins provide potential urinary biomarkers for BDU activity monitoring and provide valuable clues for the analysis of the pathogenic mechanisms of BDU.
Identifiants
pubmed: 38982370
doi: 10.1186/s12886-024-03557-9
pii: 10.1186/s12886-024-03557-9
doi:
Substances chimiques
Biomarkers
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
277Subventions
Organisme : National Natural Science Foundation of China
ID : 82000881
Organisme : Beijing Natural Science Foundation
ID : 7192174
Organisme : Beijing Natural Science Foundation
ID : 7192174
Organisme : Clinical Research Fund of Beijing Municipal Science and Technology Commission
ID : Z171100001017217
Informations de copyright
© 2024. The Author(s).
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