Connexin 30 locally controls actin cytoskeleton and mechanical remodeling in motile astrocytes.
actin
astrocyte
connexin
motility
physical surface properties
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
09 Jul 2024
09 Jul 2024
Historique:
revised:
04
06
2024
received:
07
06
2022
accepted:
25
06
2024
medline:
10
7
2024
pubmed:
10
7
2024
entrez:
10
7
2024
Statut:
aheadofprint
Résumé
During brain maturation, astrocytes establish complex morphologies unveiling intense structural plasticity. Connexin 30 (Cx30), a gap-junction channel-forming protein expressed postnatally, dynamically regulates during development astrocyte morphological properties by controlling ramification and extension of fine processes. However, the underlying mechanisms remain unexplored. Here, we found in vitro that Cx30 interacts with the actin cytoskeleton in astrocytes and inhibits its structural reorganization and dynamics during cell migration. This translates into an alteration of local physical surface properties, as assessed by correlative imaging using stimulated emission depletion (STED) super resolution imaging and atomic force microscopy (AFM). Specifically, Cx30 impaired astrocyte cell surface topology and cortical stiffness in motile astrocytes. As Cx30 alters actin organization, dynamics, and membrane physical properties, we assessed whether it controls astrocyte migration. We found that Cx30 reduced persistence and directionality of migrating astrocytes. Altogether, these data reveal Cx30 as a brake for astrocyte structural and mechanical plasticity.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : MR/K015850/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K02292X/1
Pays : United Kingdom
Organisme : Engineering and Physical Sciences Research Council
ID : EP/H018301/1
Organisme : Engineering and Physical Sciences Research Council
ID : EP/L015889/1
Organisme : The Wellcome Trust
ID : 089703/Z/09/Z
Organisme : The Wellcome Trust
ID : 3-3249/Z/16/Z
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 722053
Organisme : FP7 Ideas: European Research Council
ID : 683154
Informations de copyright
© 2024 The Author(s). GLIA published by Wiley Periodicals LLC.
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