Implications of silver nanoparticles for
Helicobacter pylori
/ drug effects
Bacterial Proteins
/ metabolism
Antigens, Bacterial
/ metabolism
Silver
/ pharmacology
Humans
Metal Nanoparticles
Helicobacter Infections
/ microbiology
Signal Transduction
/ drug effects
Host-Pathogen Interactions
Epithelial Cells
/ microbiology
Virulence Factors
/ metabolism
Cell Line
Anti-Bacterial Agents
/ pharmacology
Cell Line, Tumor
AGS
CagA
H. pylori
IL-8
infection
minimum inhibitory concentration
silver nanoparticles
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2024
2024
Historique:
received:
18
04
2024
accepted:
10
06
2024
medline:
10
7
2024
pubmed:
10
7
2024
entrez:
10
7
2024
Statut:
epublish
Résumé
Helicobacter pylori infection poses a significant health burden worldwide, and its virulence factor CagA plays a pivotal role in its pathogenesis. In this study, the interaction between H. pylori-infected AGS cells and silver nanoparticles (AgNPs) was investigated, with a focus on the modulation of CagA-mediated responses, investigated by western blotting. Both, the dose-dependent efficacy against H. pylori (growth curves, CFU assay) and the impact of the nanoparticles on AGS cells (MTT assay) were elucidated. AGS cells infected with H. pylori displayed dramatic morphological changes, characterized by elongation and a migratory phenotype, attributed to CagA activity. Preincubation of H. pylori with AgNPs affected these morphological changes in a concentration-dependent manner, suggesting a correlation between AgNPs concentration and CagA function. Our study highlights the nuanced interplay between host-pathogen interactions and the therapeutic potential of AgNPs in combating H. pylori infection and offers valuable insights into the multifaceted dynamics of CagA mediated responses.
Sections du résumé
Background
UNASSIGNED
Helicobacter pylori infection poses a significant health burden worldwide, and its virulence factor CagA plays a pivotal role in its pathogenesis.
Methods
UNASSIGNED
In this study, the interaction between H. pylori-infected AGS cells and silver nanoparticles (AgNPs) was investigated, with a focus on the modulation of CagA-mediated responses, investigated by western blotting. Both, the dose-dependent efficacy against H. pylori (growth curves, CFU assay) and the impact of the nanoparticles on AGS cells (MTT assay) were elucidated.
Results
UNASSIGNED
AGS cells infected with H. pylori displayed dramatic morphological changes, characterized by elongation and a migratory phenotype, attributed to CagA activity. Preincubation of H. pylori with AgNPs affected these morphological changes in a concentration-dependent manner, suggesting a correlation between AgNPs concentration and CagA function.
Conclusion
UNASSIGNED
Our study highlights the nuanced interplay between host-pathogen interactions and the therapeutic potential of AgNPs in combating H. pylori infection and offers valuable insights into the multifaceted dynamics of CagA mediated responses.
Identifiants
pubmed: 38983115
doi: 10.3389/fcimb.2024.1419568
pmc: PMC11231068
doi:
Substances chimiques
cagA protein, Helicobacter pylori
0
Bacterial Proteins
0
Antigens, Bacterial
0
Silver
3M4G523W1G
Virulence Factors
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1419568Informations de copyright
Copyright © 2024 Hochvaldova, Posselt, Wessler, Kvítek and Panáček.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.