An HSF1-JMJD6-HSP feedback circuit promotes cell adaptation to proteotoxic stress.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
16 Jul 2024
Historique:
medline: 10 7 2024
pubmed: 10 7 2024
entrez: 10 7 2024
Statut: ppublish

Résumé

Heat Shock Factor 1 (HSF1) is best known as the master transcriptional regulator of the heat-shock response (HSR), a conserved adaptive mechanism critical for protein homeostasis (proteostasis). Combining a genome-wide RNAi library with an HSR reporter, we identified Jumonji domain-containing protein 6 (JMJD6) as an essential mediator of HSF1 activity. In follow-up studies, we found that JMJD6 is itself a noncanonical transcriptional target of HSF1 which acts as a critical regulator of proteostasis. In a positive feedback circuit, HSF1 binds and promotes JMJD6 expression, which in turn reduces heat shock protein 70 (HSP70) R469 monomethylation to disrupt HSP70-HSF1 repressive complexes resulting in enhanced HSF1 activation. Thus, JMJD6 is intricately wired into the proteostasis network where it plays a critical role in cellular adaptation to proteotoxic stress.

Identifiants

pubmed: 38985769
doi: 10.1073/pnas.2313370121
doi:

Substances chimiques

Heat Shock Transcription Factors 0
HSF1 protein, human 0
Jumonji Domain-Containing Histone Demethylases EC 1.14.11.-
HSP70 Heat-Shock Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2313370121

Subventions

Organisme : Foundation for the National Institutes of Health (FNIH)
ID : F30CA264513
Organisme : NIGMS NIH HHS
ID : R01 GM144617
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA070085
Pays : United States
Organisme : HHS | NIH | National Cancer Institute (NCI)
ID : R50CA221848
Organisme : Foundation for the National Institutes of Health (FNIH)
ID : 1R01GM144617-01

Déclaration de conflit d'intérêts

Competing interests statement:The authors declare no competing interest.

Auteurs

Milad J Alasady (MJ)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Martina Koeva (M)

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Massachusetts Institute of Technology, Cambridge, MA 02142.
HHMI, Cambridge, MA 02139.

Seesha R Takagishi (SR)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Dmitri Segal (D)

Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.

David R Amici (DR)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Roger S Smith (RS)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Daniel J Ansel (DJ)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Susan Lindquist (S)

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Massachusetts Institute of Technology, Cambridge, MA 02142.
HHMI, Cambridge, MA 02139.

Luke Whitesell (L)

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Massachusetts Institute of Technology, Cambridge, MA 02142.

Elizabeth T Bartom (ET)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Mikko Taipale (M)

Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.

Marc L Mendillo (ML)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Simpson Querrey Center for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.
Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

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Classifications MeSH