Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
10 Jul 2024
10 Jul 2024
Historique:
received:
12
01
2022
accepted:
31
05
2024
medline:
11
7
2024
pubmed:
11
7
2024
entrez:
10
7
2024
Statut:
aheadofprint
Résumé
Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools are designed to study neurons and are not suitable for controlling calcium signals in astrocytes. Here, we show that electrical stimulation of astrocytes using electrodes coated with graphene oxide and reduced graphene oxide induces respectively a slow response to calcium, mediated by external calcium influx, and a sharp one, exclusively due to calcium release from intracellular stores. Our results suggest that the different conductivities of the substrate influence the electric field at the cell-electrolyte or cell-material interfaces, favouring different signalling events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium imaging data support the proposed model. In summary, we provide evidence of a simple tool to selectively control distinct calcium signals in brain astrocytes for straightforward investigations in neuroscience and bioelectronic medicine.
Identifiants
pubmed: 38987650
doi: 10.1038/s41565-024-01711-4
pii: 10.1038/s41565-024-01711-4
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-20-1-0386
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-23-1-0736
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-21-1-0424
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-20-1-0386
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-23-1-0736
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-20-1-0386
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-21-1-0424
Organisme : United States Department of Defense | United States Air Force | AFMC | Air Force Office of Scientific Research (AF Office of Scientific Research)
ID : FA9550-20-1-0386
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca (Ministry of Education, University and Research)
ID : PRIN-PNRR-NANODYNE
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca (Ministry of Education, University and Research)
ID : PNRR MUR project ECS_00000033_ECOSISTER
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca (Ministry of Education, University and Research)
ID : PNRR MUR project ECS_00000033_ECOSISTER
Organisme : European Commission (EC)
ID : GA956325
Organisme : European Commission (EC)
ID : Graphene Flagship- GA 20-05770S
Organisme : European Commission (EC)
ID : Graphene Flagship
Organisme : European Commission (EC)
ID : GA956325
Organisme : European Commission (EC)
ID : GA956325
Organisme : European Commission (EC)
ID : GA956325
Organisme : European Commission (EC)
ID : GrapheneCore3 881603 - Graphene Flagship
Organisme : European Commission (EC)
ID : GrapheneCore3 881603 - Graphene Flagship
Organisme : Akademie Věd České Republiky (Academy of Sciences of the Czech Republic)
ID : 20-05770S
Informations de copyright
© 2024. The Author(s).
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