Fluorescent DNA tetrahedral probe with catalytic hairpin self-assembly reaction for imaging of miR-21 and miR-155 in living cells.
Catalytic hairpin assembly
Fluorescence resonance energy transfer
Fluorescent DNA tetrahedral probe
Living cell imaging
MicroRNAs
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
11 Jul 2024
11 Jul 2024
Historique:
received:
29
03
2024
accepted:
26
06
2024
medline:
11
7
2024
pubmed:
11
7
2024
entrez:
11
7
2024
Statut:
epublish
Résumé
A CHA-based fluorescent DNA tetrahedral probe (FDTp) has been designed to detect the microRNAs miR-21 and miR-155 sensitively and specifically in living cells. The design consisted of functional elements (H1, H2, and Protector) connected to a DNA tetrahedron modified with two pairs of fluorophores and quenching groups. In the presence of miR-21, the chain displacement effect was triggered and Cy3 fluorescence was emitted. In the presence of miR-155, the signal of the catalytic hairpin assembly (CHA) between H1 and H2 on FDTp was amplified, making the fluorescence of FAM sensitive to miR-155. Using this method, the detection limit for miR-155 was 5 pM. The FDTp successfully imaged miR-21 and miR-155 in living cells and distinguished a variety of cell lines based on their expression levels of miR-21 and miR-155. The detection and imaging of dual targets in this design ensured the accuracy of tumor diagnosis and provided a new method for early tumor diagnosis.
Identifiants
pubmed: 38990374
doi: 10.1007/s00604-024-06529-4
pii: 10.1007/s00604-024-06529-4
doi:
Substances chimiques
MicroRNAs
0
MIRN21 microRNA, human
0
MIRN155 microRNA, human
0
Fluorescent Dyes
0
DNA Probes
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
462Subventions
Organisme : National Natural Science Foundation of China
ID : 82230070
Organisme : National Natural Science Foundation of China
ID : 82072058
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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