Mmp14-dependent remodeling of the pericellular-dermal collagen interface governs fibroblast survival.


Journal

The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356

Informations de publication

Date de publication:
02 Sep 2024
Historique:
received: 18 12 2023
revised: 10 05 2024
accepted: 30 05 2024
medline: 11 7 2024
pubmed: 11 7 2024
entrez: 11 7 2024
Statut: ppublish

Résumé

Dermal fibroblasts deposit type I collagen, the dominant extracellular matrix molecule found in skin, during early postnatal development. Coincident with this biosynthetic program, fibroblasts proteolytically remodel pericellular collagen fibrils by mobilizing the membrane-anchored matrix metalloproteinase, Mmp14. Unexpectedly, dermal fibroblasts in Mmp14-/- mice commit to a large-scale apoptotic program that leaves skin tissues replete with dying cells. A requirement for Mmp14 in dermal fibroblast survival is recapitulated in vitro when cells are embedded within, but not cultured atop, three-dimensional hydrogels of crosslinked type I collagen. In the absence of Mmp14-dependent pericellular proteolysis, dermal fibroblasts fail to trigger β1 integrin activation and instead actuate a TGF-β1/phospho-JNK stress response that leads to apoptotic cell death in vitro as well as in vivo. Taken together, these studies identify Mmp14 as a requisite cell survival factor that maintains dermal fibroblast viability in postnatal dermal tissues.

Identifiants

pubmed: 38990714
pii: 276851
doi: 10.1083/jcb.202312091
pii:
doi:

Substances chimiques

Matrix Metalloproteinase 14 EC 3.4.24.80
Mmp14 protein, mouse 0
Collagen Type I 0
Integrin beta1 0
Transforming Growth Factor beta1 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIH HHS
ID : R01-CA-071699-16-A1
Pays : United States
Organisme : Breast Cancer Research Foundation
Organisme : Margolies Family Discovery Fund for Cancer Research

Informations de copyright

© 2024 Sabeh et al.

Auteurs

Farideh Sabeh (F)

Division of Genetic Medicine, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Xiao-Yan Li (XY)

Division of Genetic Medicine, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Adam W Olson (AW)

Division of Genetic Medicine, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Elliot Botvinick (E)

The Henry Samueli School of Engineering, University of California , Irvine, CA, USA.

Abhishek Kurup (A)

The Henry Samueli School of Engineering, University of California , Irvine, CA, USA.

Luis E Gimenez (LE)

Life Sciences Institute, University of Michigan , Ann Arbor, MI, USA.

Jung-Sun Cho (JS)

Division of Genetic Medicine, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Stephen J Weiss (SJ)

Division of Genetic Medicine, Department of Internal Medicine, Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

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Classifications MeSH