HIV Subtypes and Drug-resistance-associated Mutations in US Blood Donors, 2015-2020.
HIV
blood donors
blood screening
genetic sequence analysis
phylogenetics
Journal
Open forum infectious diseases
ISSN: 2328-8957
Titre abrégé: Open Forum Infect Dis
Pays: United States
ID NLM: 101637045
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
received:
02
04
2024
accepted:
14
06
2024
medline:
12
7
2024
pubmed:
12
7
2024
entrez:
12
7
2024
Statut:
epublish
Résumé
Monitoring genotypes of HIV infections in blood donors may provide insights into infection trends in the general population. HIV RNA was extracted from plasma samples of blood donors confirmed as HIV positive by blood screening nucleic acid and antibody tests. HIV genome target regions were amplified using nested real time-polymerase chain reaction followed by next-generation sequencing. Sequences were compared to those in the Los Alamos National Laboratory (LANL) database. Sequences were also assessed for drug resistance mutations (DRM) using the Stanford HIV DRM Database. From available HIV-positive donations collected between 1 September 2015 and 31 December 2020, 563 of 743 (75.8%) were successfully sequenced; 4 were subtype A, 543 subtype B, 5 subtype C, 1 subtype G, 5 circulating recombinant forms (CRF), and 2 were subtype B and D recombinants. Overall, no significant differences between blood donor and available LANL genotypes were found, and the genotypes of newly acquired versus prevalent HIV infections in donors were similar. The proportion of non-B subtypes and CRF remained a small fraction, with no other subtype or CRF representing more than 1% of the total. DRM were identified in 122 (21.6%) samples with protease inhibitor, nucleoside reverse transcriptase inhibitor and non-nucleoside reverse transcriptase inhibitor DRMs identified in 4.9%, 4.6% and 14.0% of samples, respectively. HIV genetic diversity and DRM in blood donors appear representative of circulating HIV infections in the US general population and may provide more information on infection diversity than sequences reported to LANL, particularly for recently transmitted infections.
Sections du résumé
Background
UNASSIGNED
Monitoring genotypes of HIV infections in blood donors may provide insights into infection trends in the general population.
Methods
UNASSIGNED
HIV RNA was extracted from plasma samples of blood donors confirmed as HIV positive by blood screening nucleic acid and antibody tests. HIV genome target regions were amplified using nested real time-polymerase chain reaction followed by next-generation sequencing. Sequences were compared to those in the Los Alamos National Laboratory (LANL) database. Sequences were also assessed for drug resistance mutations (DRM) using the Stanford HIV DRM Database.
Results
UNASSIGNED
From available HIV-positive donations collected between 1 September 2015 and 31 December 2020, 563 of 743 (75.8%) were successfully sequenced; 4 were subtype A, 543 subtype B, 5 subtype C, 1 subtype G, 5 circulating recombinant forms (CRF), and 2 were subtype B and D recombinants. Overall, no significant differences between blood donor and available LANL genotypes were found, and the genotypes of newly acquired versus prevalent HIV infections in donors were similar. The proportion of non-B subtypes and CRF remained a small fraction, with no other subtype or CRF representing more than 1% of the total. DRM were identified in 122 (21.6%) samples with protease inhibitor, nucleoside reverse transcriptase inhibitor and non-nucleoside reverse transcriptase inhibitor DRMs identified in 4.9%, 4.6% and 14.0% of samples, respectively.
Conclusions
UNASSIGNED
HIV genetic diversity and DRM in blood donors appear representative of circulating HIV infections in the US general population and may provide more information on infection diversity than sequences reported to LANL, particularly for recently transmitted infections.
Identifiants
pubmed: 38994445
doi: 10.1093/ofid/ofae343
pii: ofae343
pmc: PMC11237352
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ofae343Investigateurs
E Notari
(E)
S Stramer
(S)
R Dodd
(R)
G Conti
(G)
R Fayed
(R)
D Nelson
(D)
R Townsend
(R)
G Foster
(G)
J Haynes
(J)
E Crawford
(E)
E Huseynova
(E)
D Krysztof
(D)
D Burke
(D)
M Lanteri
(M)
V Green
(V)
S Cyrus
(S)
P Williamson
(P)
D Kessler
(D)
J Gorlin
(J)
L Milan-Benson
(L)
C DelValle
(C)
P Chien
(P)
T Brown
(T)
R Reik
(R)
C Shea
(C)
M Lopez
(M)
K Richards
(K)
T Foster
(T)
J Brodsky
(J)
M Barr
(M)
T Rains
(T)
B Custer
(B)
R Bruhn
(R)
E Grebe
(E)
M Busch
(M)
M Stone
(M)
C Di Germanio
(C)
D Hindes
(D)
Z Kaidarova
(Z)
K Zurita
(K)
A Tadena
(A)
L Montalvo
(L)
A Dayana
(A)
S Hughes
(S)
M Townsend
(M)
M Bravo
(M)
J Vannoy
(J)
S Fallon
(S)
S Anderson
(S)
B Whitaker
(B)
H Yang
(H)
A Belov
(A)
A Eder
(A)
B Hailu
(B)
S Zou
(S)
J Berger
(J)
Brian Custer
(B)
Susan Stramer
(S)
Debra Kessler
(D)
Rita Reik
(R)
Phillip Williamson
(P)
Steven A Anderson
(SA)
Benyam Hailu
(B)
Informations de copyright
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
Déclaration de conflit d'intérêts
Potential conflicts of interest. The authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest.