High resolution imaging of human development: shedding light on contrast agents.

Contrast agents High-resolution imaging techniques Micro-CT Microvascular tissue imaging Vascular development

Journal

Neuroradiology
ISSN: 1432-1920
Titre abrégé: Neuroradiology
Pays: Germany
ID NLM: 1302751

Informations de publication

Date de publication:
12 Jul 2024
Historique:
received: 01 03 2024
accepted: 21 06 2024
medline: 12 7 2024
pubmed: 12 7 2024
entrez: 12 7 2024
Statut: aheadofprint

Résumé

Visualizing (micro)vascular structures remains challenging for researchers and clinicians due to limitations in traditional radiological imaging methods. Exploring the role of vascular development in craniofacial malformations in experimental settings can enhance understanding of these processes, with the effectiveness of high-resolution imaging techniques being crucial for successful research in this field. Micro-CT imaging offers 3D microstructural insights, but requires contrast-enhancing staining agents (CESAs) for visualizing (micro)-vascular tissues, known as contrast-enhanced micro-CT (CECT). As effective contrast agents are crucial for optimal visualization, this review focuses on comparative studies investigating such agents for micro-vascular tissue imaging using micro-CT. Furthermore, we demonstrate the utilization of B-Lugol solution as a promising contrast agent for acquiring high-quality micro-CT images of (micro)vascular structures in human embryonic samples. This scoping review followed Preferred Reporting Items for Systematic Reviews and Meta-analysis Protocols. PubMed database provided relevant articles, screened initially by title and abstract. Inclusion and exclusion criteria defined outcomes of interest. From an initial search, 273 records were identified, narrowed down to 9 articles after applying our criteria. Additionally, two articles were added through citation searching. This, a total of 11 articles were incorporated in this study. This micro-CT contrast agent review underscores the need for tailored choices based on research goals. Both Barium sulfate and Iodine-based agents showing excellent results, providing high resolution (micro) vascular content, especially in ex-vivo specimens. However, careful consideration of protocols and tissue characteristics remains imperative for optimizing the effectiveness of micro-CT imaging for the study of cranio-facial vascular development.

Sections du résumé

BACKGROUND BACKGROUND
Visualizing (micro)vascular structures remains challenging for researchers and clinicians due to limitations in traditional radiological imaging methods. Exploring the role of vascular development in craniofacial malformations in experimental settings can enhance understanding of these processes, with the effectiveness of high-resolution imaging techniques being crucial for successful research in this field. Micro-CT imaging offers 3D microstructural insights, but requires contrast-enhancing staining agents (CESAs) for visualizing (micro)-vascular tissues, known as contrast-enhanced micro-CT (CECT). As effective contrast agents are crucial for optimal visualization, this review focuses on comparative studies investigating such agents for micro-vascular tissue imaging using micro-CT. Furthermore, we demonstrate the utilization of B-Lugol solution as a promising contrast agent for acquiring high-quality micro-CT images of (micro)vascular structures in human embryonic samples.
METHOD METHODS
This scoping review followed Preferred Reporting Items for Systematic Reviews and Meta-analysis Protocols. PubMed database provided relevant articles, screened initially by title and abstract. Inclusion and exclusion criteria defined outcomes of interest.
RESULTS RESULTS
From an initial search, 273 records were identified, narrowed down to 9 articles after applying our criteria. Additionally, two articles were added through citation searching. This, a total of 11 articles were incorporated in this study.
CONCLUSION CONCLUSIONS
This micro-CT contrast agent review underscores the need for tailored choices based on research goals. Both Barium sulfate and Iodine-based agents showing excellent results, providing high resolution (micro) vascular content, especially in ex-vivo specimens. However, careful consideration of protocols and tissue characteristics remains imperative for optimizing the effectiveness of micro-CT imaging for the study of cranio-facial vascular development.

Identifiants

DOI: 10.1007/s00234-024-03413-z PMID: 38995394
pubmed: 38995394
doi: 10.1007/s00234-024-03413-z
pii: 10.1007/s00234-024-03413-z
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s).

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Auteurs

Karl Jacobs (K)

Department of Orofacial Pain and Disfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands. K.jacobs@acta.nl.
Department of Medical Biology, Section Clinical Anatomy & Embryology, Amsterdam UMC location AMC, University of Amsterdam, Meibergdreef 15, Amsterdam, The Netherlands. K.jacobs@acta.nl.
Amsterdam Reproduction and Development Research Institute, Meibergdreef 9, Amsterdam, The Netherlands. K.jacobs@acta.nl.
ORCID: 0000-0002-7701-9370

Daniel Docter (D)

Department of Medical Biology, Section Clinical Anatomy & Embryology, Amsterdam UMC location AMC, University of Amsterdam, Meibergdreef 15, Amsterdam, The Netherlands.

Lotte de Smit (L)

Department of Orofacial Pain and Disfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.

Hans A M Korfage (HAM)

Department of Orofacial Pain and Disfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.

Sophie C Visser (SC)

Department of Medical Biology, Section Clinical Anatomy & Embryology, Amsterdam UMC location AMC, University of Amsterdam, Meibergdreef 15, Amsterdam, The Netherlands.

Frank Lobbezoo (F)

Department of Orofacial Pain and Disfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.

Ruslan Hlushchuk (R)

Micro-CT Research Group, Institute of Anatomy, University of Bern, Baltzerstrasse 2, CH-3012, Bern, Switzerland.

Bernadette S de Bakker (BS)

Amsterdam Reproduction and Development Research Institute, Meibergdreef 9, Amsterdam, The Netherlands.
Department of Obstetrics and Gynecology, Amsterdam UMC location AMC, University of Amsterdam, Amsterdam Reproduction & Development Research Institute, Meibergdreef 9, Amsterdam, The Netherlands.
Erasmus MC - Sophia Children's Hospital, University Medical Center Rotterdam, Department of Pediatric Surgery, Rotterdam, The Netherlands.

Classifications MeSH