Sequential Double Immunoblotting with Peptide Antibodies.
Calreticulin
Double immunoblotting
Peptide antibodies
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
13
7
2024
pubmed:
13
7
2024
entrez:
12
7
2024
Statut:
ppublish
Résumé
Immunoblotting, also termed western blotting, is a powerful method for detection and characterization of proteins separated by various electrophoretic techniques. The combination of sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE), having high separating power, immunoblotting to synthetic membranes, and detection with highly specific peptide antibodies, is especially useful for studying individual proteins in relation to cellular processes, disease mechanisms, etc. Here, we describe a protocol for the sequential detection of various forms of an individual protein using peptide antibodies, exemplified by the characterization of antibody specificity for different forms of the protein calreticulin by double SDS-PAGE immunoblotting.
Identifiants
pubmed: 38997494
doi: 10.1007/978-1-0716-3914-6_19
doi:
Substances chimiques
Peptides
0
Antibodies
0
Calreticulin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
237-248Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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