CITED1 expression in odontogenic cysts.
CITED1
Dentigerous cyst
Keratocyst
Odontogenic cyst
Radicular cyst
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
12 Jul 2024
12 Jul 2024
Historique:
received:
06
03
2024
accepted:
27
05
2024
medline:
13
7
2024
pubmed:
13
7
2024
entrez:
12
7
2024
Statut:
epublish
Résumé
Originating from odontogenic tissue, Odontogenic cysts are pathological cavities lined with epithelial cells and surrounded by fibrous connective tissue. This study investigated expression of CITED1 protein in different types of odontogenic cysts. 40 keratocysts, 40 radicular cysts, and 40 dentigerous cysts were excised and processed for routine paraffin wax embedding protocol. Macroscopic and panoramic radiographies images were used for diagnosis. Demographical properties and dental parameters were recorded. Cystic tissues were stained with hematoxylin-eosin dye and CITED1 antibody. Semi-quantitative analysis was performed for immune staining. The protein-protein interaction network, hub gene detection and KEGG analysis were conducted using Cytoscape software. Odontogenic keratocysts was imaged with 6-8 layered epithelial cells and fibrous cyst walls with inflammatory cells. Radicular cysts had stratified squamous epithelium with varying thickness, ciliated cells, and Rushton hyaline bodies. Dentigerous cysts presented hyperplastic non-keratinized epithelium, fibrous tissue, rete ridges, and inflammatory cells. CITED1 immunoexpression was highest in odontogenic keratocysts, followed by radicular cysts, and lowest in dentigerous cysts. Nuclear and cytoplasmic CITED1 expression was significantly elevated in odontogenic keratocysts compared to radicular and dentigerous cysts. The top five targets of CITED1 were identified, primarily showing enrichment in hormone and cancer related pathways. Positive CITED1 expression in all three types of odontogenic cysts suggest a potential role for CITED1 in the pathogenesis of odontogenic cysts, particularly in keratocysts. Further investigations are needed to elucidate the exact mechanisms underlying the differential expression of CITED1 and its implications for the development and progression of odontogenic cysts.
Sections du résumé
BACKGROUND
BACKGROUND
Originating from odontogenic tissue, Odontogenic cysts are pathological cavities lined with epithelial cells and surrounded by fibrous connective tissue. This study investigated expression of CITED1 protein in different types of odontogenic cysts.
MATERIAL AND METHOD
METHODS
40 keratocysts, 40 radicular cysts, and 40 dentigerous cysts were excised and processed for routine paraffin wax embedding protocol. Macroscopic and panoramic radiographies images were used for diagnosis. Demographical properties and dental parameters were recorded. Cystic tissues were stained with hematoxylin-eosin dye and CITED1 antibody. Semi-quantitative analysis was performed for immune staining. The protein-protein interaction network, hub gene detection and KEGG analysis were conducted using Cytoscape software.
RESULT
RESULTS
Odontogenic keratocysts was imaged with 6-8 layered epithelial cells and fibrous cyst walls with inflammatory cells. Radicular cysts had stratified squamous epithelium with varying thickness, ciliated cells, and Rushton hyaline bodies. Dentigerous cysts presented hyperplastic non-keratinized epithelium, fibrous tissue, rete ridges, and inflammatory cells. CITED1 immunoexpression was highest in odontogenic keratocysts, followed by radicular cysts, and lowest in dentigerous cysts. Nuclear and cytoplasmic CITED1 expression was significantly elevated in odontogenic keratocysts compared to radicular and dentigerous cysts. The top five targets of CITED1 were identified, primarily showing enrichment in hormone and cancer related pathways.
CONCLUSIONS
CONCLUSIONS
Positive CITED1 expression in all three types of odontogenic cysts suggest a potential role for CITED1 in the pathogenesis of odontogenic cysts, particularly in keratocysts. Further investigations are needed to elucidate the exact mechanisms underlying the differential expression of CITED1 and its implications for the development and progression of odontogenic cysts.
Identifiants
pubmed: 38997708
doi: 10.1186/s12903-024-04413-4
pii: 10.1186/s12903-024-04413-4
doi:
Substances chimiques
Trans-Activators
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
782Informations de copyright
© 2024. The Author(s).
Références
Ansari MK, Alam S, Meraj F, Ahmed SS, Munir SAK. Clinicopathological analysis of 847 odontogenic cysts in north Indian population examined over 10 years’ period: a retrospective study. Indian J Oral Health Res. 2020;6(2):50.
Soluk-Tekkesin M. JM Wright 2022 The World Health Organization Classification of Odontogenic Lesions: a Summary of the changes of the 2022 (5th) Edition. Turk Patoloji Derg 38 2 168–84.
pubmed: 35578902
pmcid: 9999699
Kammer PV, Mello FW, Rivero ERC. Comparative analysis between developmental and inflammatory odontogenic cysts: retrospective study and literature review. Oral Maxillofac Surg. 2020;24(1):73–84.
doi: 10.1007/s10006-019-00816-8
pubmed: 31858303
Rajendra Santosh AB. Odontogenic cysts. Dent Clin North Am. 2020;64(1):105–19.
doi: 10.1016/j.cden.2019.08.002
pubmed: 31735221
Brown SJ, Conn BI. Odontogenic cysts: classification, histological features and a practical approach to common diagnostic problems. Diagn Histopathology. 2022;28(5):253–66.
doi: 10.1016/j.mpdhp.2022.02.007
Jones AV, Craig GT, Franklin CD. Range and demographics of odontogenic cysts diagnosed in a UK population over a 30-year period. J Oral Pathol Med. 2006;35(8):500–7.
doi: 10.1111/j.1600-0714.2006.00455.x
pubmed: 16918602
Johnson NR, Gannon OM, Savage NW, Batstone MD. Frequency of odontogenic cysts and tumors: a systematic review. J Invest Clin Dent. 2014;5(1):9–14.
doi: 10.1111/jicd.12044
Gorlin RJ. Potentialities of oral epithelium manifest by mandibular dentigerous cysts. Oral Surg Oral Med Oral Pathol. 1957;10(3):271–84.
doi: 10.1016/0030-4220(57)90092-0
pubmed: 13407922
Robinson RA. Diagnosing the most common odontogenic cystic and osseous lesions of the jaws for the practicing pathologist. Mod Pathol. 2017;30(s1):S96–103.
doi: 10.1038/modpathol.2016.191
pubmed: 28060370
Yahata T, Shao W, Endoh H, Hur J, Coser KR, Sun H, Ueda Y, Kato S, Isselbacher KJ, Brown M, et al. Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein. Genes Dev. 2001;15(19):2598–612.
doi: 10.1101/gad.906301
pubmed: 11581164
pmcid: 312794
Prasad ML, Pellegata NS, Huang Y, Nagaraja HN, de la Chapelle A, Kloos RT. Galectin-3, fibronectin-1, CITED-1, HBME1 and cytokeratin-19 immunohistochemistry is useful for the differential diagnosis of thyroid tumors. Mod Pathol. 2005;18(1):48–57.
doi: 10.1038/modpathol.3800235
pubmed: 15272279
Rodriguez TA, Sparrow DB, Scott AN, Withington SL, Preis JI, Michalicek J, Clements M, Tsang TE, Shioda T, Beddington RS, et al. Cited1 is required in trophoblasts for placental development and for embryo growth and survival. Mol Cell Biol. 2004;24(1):228–44.
doi: 10.1128/MCB.24.1.228-244.2004
pubmed: 14673158
pmcid: 303371
Shioda T, Lechleider RJ, Dunwoodie SL, Li H, Yahata T, de Caestecker MP, Fenner MH, Roberts AB, Isselbacher KJ. Transcriptional activating activity of Smad4: roles of SMAD hetero-oligomerization and enhancement by an associating transactivator. Proc Natl Acad Sci U S A. 1998;95(17):9785–90.
doi: 10.1073/pnas.95.17.9785
pubmed: 9707553
pmcid: 21414
Dunwoodie SL, Rodriguez TA, Beddington RS. Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. Mech Dev. 1998;72(1–2):27–40.
doi: 10.1016/S0925-4773(98)00011-2
pubmed: 9533950
Sun Y, Guo HH, Guo DD, Jiang XY, Zou SM. Divergence of genes encoding CITED1 and CITED2 in Blunt Snout Bream (Megalobrama amblycephala) and their transcriptional responses to Hypoxia. Front Physiol. 2018;9:186.
doi: 10.3389/fphys.2018.00186
pubmed: 29559927
pmcid: 5845555
Gordeeff M, Clergeau-Guerithault S, Gordeeff A. [Histopathology of odontogenic cysts]. J Biol Buccale. 1989;17(3):131–46.
pubmed: 2681180
Nayyer NV, Macluskey M, Keys W. Odontogenic cysts - an overview. Dent Update. 2015;42(6):548–51. 553 – 545.
doi: 10.12968/denu.2015.42.6.548
pubmed: 26506810
Al Sheddi MA. Odontogenic cysts. A clinicopathological study. Saudi Med J. 2012;33(3):304–8.
pubmed: 22426912
Fatih T, Erdemci F, Fırat A, MARAŞLI M, Deveci E. Histopathological examination of the placenta after delivery in pregnant women with COVID-19. J Health Sci Med, 5(3):868–74.
Crowe AR, Yue W. Semi-quantitative determination of protein expression using immunohistochemistry staining and analysis: an Integrated Protocol. Bio Protoc. 2019;9(24):e3465.
doi: 10.21769/BioProtoc.3465
pubmed: 31867411
pmcid: 6924920
Aşır F, Oğlak SC, Ağaçayak E, Alabalık U. Homeobox a cluster 7 (HOXA7) protein expression increased in the placentas of patients with preterm delivery. Perinat J. 2023;31(3):213–8.
Keşim DA, Aşır F, Ayaz H, Korak T. The effects of Ellagic Acid on Experimental Corrosive Esophageal burn Injury. Curr Issues Mol Biol. 2024;46(2):1579–92.
doi: 10.3390/cimb46020102
pubmed: 38392220
pmcid: 10888482
Chin C-H, Chen S-H, Wu H-H, Ho C-W, Ko M-T, Lin C-Y. cytoHubba: identifying hub objects and sub-networks from complex interactome. BMC Syst Biol. 2014;8(4):S11.
doi: 10.1186/1752-0509-8-S4-S11
pubmed: 25521941
pmcid: 4290687
Murphy AJ, de Caestecker C, Pierce J, Boyle SC, Ayers GD, Zhao Z, Libes JM, Correa H, Walter T, Huppert SS, et al. CITED1 expression in liver development and hepatoblastoma. Neoplasia. 2012;14(12):1153–63.
doi: 10.1593/neo.12958
pubmed: 23308048
pmcid: 3540941
Gomes IP, Bastos VC, Guimarães LM, Gomes CC. The molecular basis of odontogenic cysts and tumours. J Oral Pathol Med. 2023;52(4):351–6.
doi: 10.1111/jop.13401
pubmed: 36629457
Tsuneki M, Yamazaki M, Cheng J, Maruyama S, Kobayashi T, Saku T. Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions: its practical use in surgical pathology. Histopathology. 2010;57(6):806–13.
doi: 10.1111/j.1365-2559.2010.03712.x
pubmed: 21166695
Wright JM, Odell EW, Speight PM, Takata T. Odontogenic tumors, WHO 2005: where do we go from here? Head Neck Pathol. 2014;8(4):373–82.
doi: 10.1007/s12105-014-0585-x
pubmed: 25409849
pmcid: 4245407
Dahlgren M, Lettiero B, Dalal H, Mårtensson K, Gaber A, Nodin B, Gruvberger-Saal SK, Saal LH, Howlin J. CITED1 as a marker of favourable outcome in anti-endocrine treated, estrogen-receptor positive, lymph-node negative breast cancer. BMC Res Notes. 2023;16(1):105.
doi: 10.1186/s13104-023-06376-1
pubmed: 37322548
pmcid: 10268435
Xu Y, Luo X, Fang Z, Zheng X, Zeng Y, Zhu C, Gu J, Tang F, Hu Y, Hu G, et al. Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling. Cell Death Dis. 2018;9(9):924.
doi: 10.1038/s41419-018-0991-1
pubmed: 30206204
pmcid: 6134011
Erkılınç G, Kulaçoğlu S. The expression of HBME1, PAX8, CD56 and CITED1 in Benign lesions, Benign and malignant tumors of thyroid. Acta Oncol Turc, 54(2):141–51.
Huang C, Sheng Y, Jia J, Chen L. Identification of melanoma biomarkers based on network modules by integrating the human signaling network with microarrays. J Cancer Res Ther. 2014;10(Suppl):C114–124.
pubmed: 25450268
Subramani A, Hite MEL, Garcia S, Maxwell J, Kondee H, Millican GE, McClelland EE, Seipelt-Thiemann RL, Nelson DE. Regulation of macrophage IFNγ-stimulated gene expression by the transcriptional coregulator CITED1. J Cell Sci 2023, 136(1).
Canassa B, Pavan A. Inflammatory odontogenic cysts: a brief literature review. J Surg Clin Dentistry-JSCD. 2014;2(1):20–8.
Hamied MA-S, Al-Shaikhani SM, Ali ZD. Inflammatory odontogenic cysts. AL-Kindy Coll Med J. 2021;17(3):135–44.
doi: 10.47723/kcmj.v17i3.260
Ibrahim IBM, Pidaparti R. Influence of pathogens and mechanical stimuli in inflammation. Bioeng (Basel) 2019, 6(2).
Lasa M, Contreras-Jurado C. Thyroid hormones act as modulators of inflammation through their nuclear receptors. Front Endocrinol (Lausanne). 2022;13:937099.
doi: 10.3389/fendo.2022.937099
pubmed: 36004343
Bowman-Colin C, Salazar LA, Martins JO. The Role of Endocrine System in the Inflammatory Process. Mediators Inflamm 2016, 2016:6081752.
Harding AT, Heaton NS. The impact of Estrogens and their receptors on immunity and inflammation during infection. Cancers (Basel) 2022, 14(4).
Greten FR, Grivennikov SI. Inflammation and Cancer: triggers, mechanisms, and consequences. Immunity. 2019;51(1):27–41.
doi: 10.1016/j.immuni.2019.06.025
pubmed: 31315034
pmcid: 6831096