Apple Pomace Extract Induces Cell Proliferation and Increases Type I Collagen and Hyaluronan Production in Human Skin Fibroblasts In Vitro.
Apple pomace extract
Collagen
Fibroblast
Hyaluronan
Skin
Journal
Plant foods for human nutrition (Dordrecht, Netherlands)
ISSN: 1573-9104
Titre abrégé: Plant Foods Hum Nutr
Pays: Netherlands
ID NLM: 8803554
Informations de publication
Date de publication:
13 Jul 2024
13 Jul 2024
Historique:
accepted:
27
06
2024
medline:
14
7
2024
pubmed:
14
7
2024
entrez:
13
7
2024
Statut:
aheadofprint
Résumé
Apple pomace is the residue left after apples are squeezed. The majority of pomace produced worldwide is produced by the apple manufacturing industry, however, most of the pomace produced by the industry is discarded. Apple pomace contains functional ingredients, such as polyphenols and triterpenoids, and exerts several beneficial effects on human health; however, studies on its cosmetic effects on the skin are lacking. Therefore, herein, we investigated the effects of apple pomace extract (APE) on human skin fibroblasts (HSFs) in vitro. When HSFs were cultured with the extract for 72 h, the number of HSFs increased at concentrations of 10 and 20 µg/mL. Transcriptome analysis and reverse transcription-quantitative PCR results revealed that the extract upregulated the expression of hyaluronan synthase (HAS) 1, HAS2, and HAS3 and downregulated the expression of HYAL1, a gene encoding the hyaluronan-degrading enzyme, in HSFs. Additionally, enzyme-linked immunosorbent assay revealed increased amounts of factors related to skin extracellular matrix, such as type I collagen and hyaluronic acid, secreted in the culture supernatant. The western blotting results suggested that the extract induced extracellular signal-regulated kinase and protein kinase B phosphorylation in HSFs. Additionally, several GO_Terms related to mitosis were detected in the Gene Ontology analysis. This is the first study to show that APE induces the proliferation of HSFs and production of factors related to skin anti-aging.
Identifiants
pubmed: 39001986
doi: 10.1007/s11130-024-01210-w
pii: 10.1007/s11130-024-01210-w
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Asif M, Javaid T, Razzaq ZU, Khan MKI, Maan AA, Yousaf S et al (2023) Sustainable utilization of apple pomace and its emerging potential for development of functional foods. Environ Sci Pollut Res Int. https://doi.org/10.1007/s11356-023-28479-9
doi: 10.1007/s11356-023-28479-9
pubmed: 37848790
Lyu F, Luiz SF, Azeredo DRP, Cruz AG, Ajlouni S, Ranadheera CS (2020) Apple Pomace as a functional and healthy ingredient in Food products: a review. Processes 8:319. https://doi.org/10.3390/pr8030319
doi: 10.3390/pr8030319
Waldbauer K, McKinnon R, Kopp B (2017) Apple Pomace as potential source of natural active compounds. Planta Med 83(12–13):994–1010. https://doi.org/10.1055/s-0043-111898
doi: 10.1055/s-0043-111898
pubmed: 28701021
Cole MA, Quan T, Voorhees JJ, Fisher GJ (2018) Extracellular matrix regulation of fibroblast function: redefining our perspective on skin aging. J Cell Commun Signal 12(1):35–43. https://doi.org/10.1007/s12079-018-0459-1
doi: 10.1007/s12079-018-0459-1
pubmed: 29455303
pmcid: 5842211
Kawada C, Yoshida T, Yoshida H, Matsuoka R, Sakamoto W, Odanaka W et al (2014) Ingested hyaluronan moisturizes dry skin. Nutr J 13:70. https://doi.org/10.1186/1475-2891-13-70
doi: 10.1186/1475-2891-13-70
pubmed: 25014997
pmcid: 4110621
Bylka W, Znajdek-Awizen P, Studzinska-Sroka E, Brzezinska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30(1):46–49. https://doi.org/10.5114/pdia.2013.33378
doi: 10.5114/pdia.2013.33378
pubmed: 24278045
pmcid: 3834700
Lee JH, Park J, Shin DW (2022) The molecular mechanism of polyphenols with anti-aging activity in aged human dermal fibroblasts. Molecules 27(14). https://doi.org/10.3390/molecules27144351
Nanashima N, Horie K, Maeda H, Tomisawa T, Kitajima M, Nakamura T (2018) Blackcurrant anthocyanins increase the Levels of Collagen, Elastin, and Hyaluronic Acid in Human skin fibroblasts and ovariectomized rats. Nutrients 10(4). https://doi.org/10.3390/nu10040495
Lee EH, Park HJ, Kim HH, Jung HY, Kang IK, Cho YJ (2021) Isolated isoquercitrin from Green ball apple peel inhibits photoaging in CCD-986Sk fibroblasts cells via modulation of the MMPs signaling. J Cosmet Dermatol 20(9):2932–2939. https://doi.org/10.1111/jocd.13903
doi: 10.1111/jocd.13903
pubmed: 33356000
Stojiljkovic D, Nesic I, Tadic V, Najman S, Stojanovic S (2022) Standardized wild apple fruit extract as a bioactive agent in dermocosmetic products for efficacy skin hydration-In vitro and in vivo evaluation. J Cosmet Dermatol 21(10):4788–4795. https://doi.org/10.1111/jocd.14787
doi: 10.1111/jocd.14787
pubmed: 35048513
Trentini M, Zanolla I, Zanotti F, Tiengo E, Licastro D, Dal Monego S et al (2022) Apple Derived exosomes improve collagen type I production and decrease MMPs during aging of the skin through downregulation of the NF-kappaB pathway as Mode of Action. Cells 11(24). https://doi.org/10.3390/cells11243950
Samina Kauser MAM, Ashiq Hussain M, Imran K, Kabir A, Najam (2024) Qurat Ul An, Saima Akram, Haya Fatima, Syeda Ayesha Batool, Anjum Shehzad, Shazia Yaqub. Apple Pomace, a bioresource of functional and nutritional components with potential of utilization in different food formulations: a review. Food Chem Adv 4:100598. https://doi.org/10.1016/j.focha.2023.100598
doi: 10.1016/j.focha.2023.100598
Watanabe A, Shimada M, Maeda H, Narumi T, Ichita J, Itoku K, Nakajima A (2024) Apple Pomace Extract improves MK-801-Induced Memory Impairment in mice. Nutrients 16(2). https://doi.org/10.3390/nu16020194
Ogawa T, Migita H, Shimada S, Ichida J, Osada K (2014) The structure and level of Glucosylceramide in Apple Pomace. Nippon Shokuhin Kagaku Kogaku Kaishi 61:251–257. https://doi.org/10.3136/nskkk.61.251
doi: 10.3136/nskkk.61.251
Fukunaga S, Wada S, Yamashita M, Morita M, Aoi W, Naito Y, Higashi A (2019) Torula yeast (Candida utilis)-derived glucosylceramide contributes to dermal elasticity in vitro. J Food Biochem 43(7):e12847. https://doi.org/10.1111/jfbc.12847
doi: 10.1111/jfbc.12847
pubmed: 31353719
Wojciak-Kosior M, Paduch R, Matysik-Wozniak A, Niedziela P, Donica H (2011) The effect of ursolic and oleanolic acids on human skin fibroblast cells. Folia Histochem Cytobiol 49(4):664–669. https://doi.org/10.5603/fhc.2011.0050
doi: 10.5603/fhc.2011.0050
pubmed: 22252762
Murphy G (2011) Tissue inhibitors of metalloproteinases. Genome Biol 12(11):233. https://doi.org/10.1186/gb-2011-12-11-233
doi: 10.1186/gb-2011-12-11-233
pubmed: 22078297
pmcid: 3334591
Cen R, Wang L, He Y, Yue C, Tan Y, Li L, Lei X (2021) Dermal fibroblast Migration and Proliferation upon Wounding or Lipopolysaccharide exposure is mediated by Stathmin. Front Pharmacol 12:781282. https://doi.org/10.3389/fphar.2021.781282
doi: 10.3389/fphar.2021.781282
pubmed: 35153746
Lavoie H, Gagnon J, Therrien M (2020) ERK signalling: a master regulator of cell behaviour, life and fate. Nat Rev Mol Cell Biol 21(10):607–632. https://doi.org/10.1038/s41580-020-0255-7
doi: 10.1038/s41580-020-0255-7
pubmed: 32576977
Manning BD, Toker A (2017) AKT/PKB signaling: navigating the network. Cell 169(3):381–405. https://doi.org/10.1016/j.cell.2017.04.001
doi: 10.1016/j.cell.2017.04.001
pubmed: 28431241
pmcid: 5546324
David-Raoudi M, Deschrevel B, Leclercq S, Galera P, Boumediene K, Pujol JP (2009) Chondroitin sulfate increases hyaluronan production by human synoviocytes through differential regulation of hyaluronan synthases: role of p38 and Akt. Arthritis Rheum 60(3):760–770. https://doi.org/10.1002/art.24302
doi: 10.1002/art.24302
pubmed: 19248106
Suto M, Masutomi H, Ishihara K, Masaki H (2019) A Potato Peel Extract stimulates type I collagen synthesis via akt and ERK Signaling in Normal Human dermal fibroblasts. Biol Pharm Bull 42(9):1510–1516. https://doi.org/10.1248/bpb.b19-00193
doi: 10.1248/bpb.b19-00193
pubmed: 31474711
Cutroneo KR (2007) TGF-beta-induced fibrosis and SMAD signaling: oligo decoys as natural therapeutics for inhibition of tissue fibrosis and scarring. Wound Repair Regen 15(Suppl 1):S54–60. https://doi.org/10.1111/j.1524-475X.2007.00226.x
doi: 10.1111/j.1524-475X.2007.00226.x
pubmed: 17727468
Vestri A, Pierucci F, Frati A, Monaco L, Meacci E (2017) Sphingosine 1-Phosphate receptors: do they have a therapeutic potential in Cardiac Fibrosis? Front Pharmacol 8:296. https://doi.org/10.3389/fphar.2017.00296
doi: 10.3389/fphar.2017.00296
pubmed: 28626422
pmcid: 5454082
Chrissouli S, Pratsinis H, Velissariou V, Anastasiou A, Kletsas D (2010) Human amniotic fluid stimulates the proliferation of human fetal and adult skin fibroblasts: the roles of bFGF and PDGF and of the ERK and akt signaling pathways. Wound Repair Regen 18(6):643–654. https://doi.org/10.1111/j.1524-475X.2010.00626.x
doi: 10.1111/j.1524-475X.2010.00626.x
pubmed: 20946138