Transcriptomic and proteomic profiling of bi-partite and tri-partite murine iPSC-derived neurospheroids under steady-state and inflammatory condition.

Inflammatory response Microglia Murine iPSC Neurospheroids Proteomics Stress granules Transcriptomics

Journal

Brain, behavior, and immunity
ISSN: 1090-2139
Titre abrégé: Brain Behav Immun
Pays: Netherlands
ID NLM: 8800478

Informations de publication

Date de publication:
11 Jul 2024
Historique:
received: 05 03 2024
revised: 24 05 2024
accepted: 06 07 2024
medline: 14 7 2024
pubmed: 14 7 2024
entrez: 13 7 2024
Statut: aheadofprint

Résumé

induced-pluripotent stem cell (iPSC)-derived neurospheroid (NSPH) models are an emerging in vitro toolkit to study the influence of inflammatory triggers on neurodegeneration and repair in a 3D neural environment. In contrast to their human counterpart, the absence of murine iPSC-derived NSPHs for profound characterisation and validation studies is a major experimental research gap, even though they offer the only possibility to truly compare or validate in vitro NSPH responses with in vivo brain responses. To contribute to these developments, we here describe the generation and characterisation of 5-week-old CX

Identifiants

DOI: 10.1016/j.bbi.2024.07.008 PMID: 39002812
pubmed: 39002812
pii: S0889-1591(24)00475-6
doi: 10.1016/j.bbi.2024.07.008
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2024 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Julia Di Stefano (J)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium; Bio-Imaging Lab, University of Antwerp, 2610 Wilrijk, Belgium.

Laura Garcia-Pupo (L)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium; Cell Death Signaling, Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610 Wilrijk, Belgium.

Federica Di Marco (FD)

Center for Advanced Studies and Technology (CAST), G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.

Helena Motaln (H)

Department of Biotechnology, Jozef Stefan Institute, SI-1000 Ljubljana, Slovenia.

Jonas Govaerts (J)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium.

Elise Van Breedam (EV)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium.

Ligia Monica Mateiu (LM)

Center for Medical Genetics, Faculty of Medicine and Health Sciences, Antwerp University Hospital, University of Antwerp, Edegem, Belgium.

Siebe Van Calster (SV)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium.

Leonardo Ricciardi (L)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium; Bio-Imaging Lab, University of Antwerp, 2610 Wilrijk, Belgium.

Alessandra Quarta (A)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium.

Peter Verstraelen (P)

Laboratory of Cell Biology and Histology and Antwerp Center for Advanced Microscopy, Department of Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium; µNEURO Research Centre of Excellence, University of Antwerp, 2610 Wilrijk, Belgium.

Winnok H De Vos (WH)

Laboratory of Cell Biology and Histology and Antwerp Center for Advanced Microscopy, Department of Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium; µNEURO Research Centre of Excellence, University of Antwerp, 2610 Wilrijk, Belgium.

Boris Rogelj (B)

Department of Biotechnology, Jozef Stefan Institute, SI-1000 Ljubljana, Slovenia.

Ilaria Cicalini (I)

Center for Advanced Studies and Technology (CAST), G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.

Vincenzo De Laurenzi (V)

Center for Advanced Studies and Technology (CAST), G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.

Piero Del Boccio (PD)

Center for Advanced Studies and Technology (CAST), G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy; Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.

Una FitzGerald (U)

CÚRAM, Centre for Research in Medical Devices, Biomedical Engineering, University of Galway, Ireland; Galway Neuroscience Centre, University of Galway, Ireland.

Wim Vanden Berghe (WV)

Cell Death Signaling, Integrated Personalized and Precision Oncology Network (IPPON), University of Antwerp, 2610 Wilrijk, Belgium.

Marleen Verhoye (M)

Bio-Imaging Lab, University of Antwerp, 2610 Wilrijk, Belgium.

Damiana Pieragostino (D)

Center for Advanced Studies and Technology (CAST), G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy.

Peter Ponsaerts (P)

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, 2610 Wilrijk, Belgium. Electronic address: peter.ponsaerts@uantwerpen.be.

Classifications MeSH