Direct Spectroscopic Ferrochelatase Assay.
Chelation
Ferrochelatase
Heme biosynthesis
Iron
Porphyrin
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
15
7
2024
pubmed:
15
7
2024
entrez:
15
7
2024
Statut:
ppublish
Résumé
Ferrochelatases (E.C. 4.99.1.1) catalyze the insertion of ferrous iron into either protoporphyrin IX to make protoheme IX or coproporphyrin III to make coproheme III. Ferrochelatase activity in extracts or purified protein can be measured via several assays. Here, we describe a rapid real-time direct spectroscopic ferrochelatase assay for both protoporphyrin and coproporphyrin ferrochelatases.
Identifiants
pubmed: 39008258
doi: 10.1007/978-1-0716-4043-2_14
doi:
Substances chimiques
Ferrochelatase
EC 4.99.1.1
Protoporphyrins
0
protoporphyrin IX
C2K325S808
Coproporphyrins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
243-247Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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