Rab10-CAV1 mediated intraluminal vesicle transport to migrasomes.
rab GTP-Binding Proteins
/ metabolism
Humans
Caveolin 1
/ metabolism
Cell Movement
Macrophages
/ metabolism
Phosphorylation
Adaptor Proteins, Signal Transducing
/ metabolism
Animals
Myosin Type V
/ metabolism
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
/ metabolism
Mice
Myosin Heavy Chains
/ metabolism
Biological Transport
Wound Healing
/ physiology
Organelles
/ metabolism
Caveolin 1
Rab GTPases
migrasome
vesicle trafficking
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
23 Jul 2024
23 Jul 2024
Historique:
medline:
15
7
2024
pubmed:
15
7
2024
entrez:
15
7
2024
Statut:
ppublish
Résumé
Migrasomes, vesicular organelles generated on the retraction fibers of migrating cells, play a crucial role in migracytosis, mediating intercellular communication. The cargoes determine the functional specificity of migrasomes. Migrasomes harbor numerous intraluminal vesicles, a pivotal component of their cargoes. The mechanism underlying the transportation of these intraluminal vesicles to the migrasomes remains enigmatic. In this study, we identified that Rab10 and Caveolin-1 (CAV1) mark the intraluminal vesicles in migrasomes. Transport of Rab10-CAV1 vesicles to migrasomes required the motor protein Myosin Va and adaptor proteins RILPL2. Notably, the phosphorylation of Rab10 by the kinase LRRK2 regulated this process. Moreover, CSF-1 can be transported to migrasomes through this mechanism, subsequently fostering monocyte-macrophage differentiation in skin wound healing, which served as a proof of the physiological importance of this transporting mechanism.
Identifiants
pubmed: 39008679
doi: 10.1073/pnas.2319267121
doi:
Substances chimiques
rab GTP-Binding Proteins
EC 3.6.5.2
Caveolin 1
0
Rab10 protein, human
EC 3.6.1.-
Adaptor Proteins, Signal Transducing
0
Myosin Type V
EC 3.6.1.-
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
EC 2.7.11.1
Myosin Heavy Chains
EC 3.6.4.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2319267121Subventions
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 32300570
Organisme : Ministry of Science and Technology of the People's Republic of China (MOST)
ID : 2022ZD0212900
Organisme : Ministry of Science and Technology of the People's Republic of China (MOST)
ID : 2023YFF0613402
Déclaration de conflit d'intérêts
Competing interests statement:The authors declare no competing interest.