Genomic surveillance of malaria parasites in an indigenous community in the Peruvian Amazon.
Peru
/ epidemiology
Humans
Plasmodium falciparum
/ genetics
Plasmodium vivax
/ genetics
Malaria, Falciparum
/ epidemiology
Malaria, Vivax
/ epidemiology
Protozoan Proteins
/ genetics
Female
Male
Child
Adult
Antimalarials
/ therapeutic use
Adolescent
Drug Resistance
/ genetics
Middle Aged
Indigenous Peoples
/ genetics
Young Adult
Child, Preschool
Genomics
/ methods
Genetic Variation
Antigens, Protozoan
/ genetics
Drug resistance
Genetic diversity
HRP2
Malaria elimination
Malaria persistence
Population genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 Jul 2024
15 Jul 2024
Historique:
received:
22
02
2024
accepted:
05
07
2024
medline:
16
7
2024
pubmed:
16
7
2024
entrez:
15
7
2024
Statut:
epublish
Résumé
Hard-to-reach communities represent Peru's main challenge for malaria elimination, but information about transmission in these areas is scarce. Here, we assessed Plasmodium vivax (Pv) and P. falciparum (Pf) transmission dynamics, resistance markers, and Pf hrp2/3 deletions in Nueva Jerusalén (NJ), a remote, indigenous community in the Peruvian Amazon with high population mobility. We collected samples from November 2019 to May 2020 by active (ACD) and passive case detection (PCD) in NJ. Parasites were identified with microscopy and PCR. Then, we analyzed a representative set of positive-PCR samples (Pv = 68, Pf = 58) using highly-multiplexed deep sequencing assays (AmpliSeq) and compared NJ parasites with ones from other remote Peruvian areas using population genetics indexes. The ACD intervention did not reduce malaria cases in the short term, and persistent malaria transmission was observed (at least one Pv infection was detected in 96% of the study days). In Nueva Jerusalen, the Pv population had modest genetic diversity (He = 0.27). Pf population had lower diversity (He = 0.08) and presented temporal clustering, one of these clusters linked to an outbreak in February 2020. Moreover, Pv and Pf parasites from NJ exhibited variable levels of differentiation (Pv Fst = 0.07-0.52 and Pf Fst = 0.11-0.58) with parasites from other remote areas. No artemisin resistance mutations but chloroquine (57%) and sulfadoxine-pyrimethamine (35-67%) were detected in NJ's Pf parasites. Moreover, pfhrp2/3 gene deletions were common (32-50% of parasites with one or both genes deleted). The persistent Pv transmission and the detection of a Pf outbreak with parasites genetically distinct from the local ones highlight the need for tailored interventions focusing on mobility patterns and imported infections in remote areas to eliminate malaria in the Peruvian Amazon.
Identifiants
pubmed: 39009685
doi: 10.1038/s41598-024-66925-x
pii: 10.1038/s41598-024-66925-x
doi:
Substances chimiques
Protozoan Proteins
0
Antimalarials
0
Antigens, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
16291Subventions
Organisme : Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica
ID : 165-2020-FONDECYT
Organisme : Belgium Development Cooperation (DGD)
ID : FA4 Peru (2017 - 2021)
Organisme : Belgium Development Cooperation (DGD)
ID : FA5 Peru (2022 - 2026)
Organisme : Armed Forces Health Surveillance Division (AFHSD-GEIS)
ID : P0134_23_N6
Organisme : NIH HHS
ID : 5U19AI089681-15
Pays : United States
Organisme : NIH HHS
ID : 5U19AI089681-15
Pays : United States
Organisme : VLIR-UOS, Belgium
ID : PE2018TEA470A102
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G.0A42.22N
Informations de copyright
© 2024. The Author(s).
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