Oxidative and carbonyl stress induced AMD and Codonopsis lanceolata ameliorates AMD via controlling oxidative and carbonyl stress.

Codonopsis / chemistry Humans Macular Degeneration / drug therapy Oxidative Stress / drug effects Apoptosis / drug effects NF-E2-Related Factor 2 / metabolism Plant Extracts / pharmacology Antioxidants / pharmacology Kelch-Like ECH-Associated Protein 1 / metabolism Cell Line Aldehydes / pharmacology Retinal Pigment Epithelium / drug effects Light / adverse effects Proto-Oncogene Proteins c-bcl-2 / metabolism
Codonopsis lanceolata 4-HNE Age-related macular degeneration (AMD) Apoptosis Keap1/Nrf2/HO-1 pathway Oxidative/carbonyl stress

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
15 Jul 2024
Historique:
received: 26 02 2024
accepted: 08 07 2024
medline: 16 7 2024
pubmed: 16 7 2024
entrez: 15 7 2024
Statut: epublish

Résumé

Age-related macular degeneration (AMD) is one of the leading causes of blindness. AMD is currently incurable; the best solution is to prevent its occurrence. To develop drugs for AMD, it is crucial to have a model system that mimics the symptoms and mechanisms in patients. It is most important to develop safer and more effective anti-AMD drug. In this study, the dose of A2E and the intensity of blue light were evaluated to establish an appropriate atrophic in vitro model of AMD and anti-AMD effect and therapeutic mechanism of Codonopsis lanceolata. The experimental groups included a control group an AMD group treated with A2E and blue light, a lutein group treated with 25 μM lutein after AMD induction, and three groups treated with different doses of C. lanceolata (10, 20, and 50 μg/mL) after AMD induction. Intrinsic apoptotic pathway (Bcl-2 family), anti-oxidative system (Keap1/Nrf2/HO-1 antioxidant response element), and anti-carbonyl effect (4-hydroxynonenal [4-HNE]) were evaluated using immunofluorescence, MTT, TUNEL, FACS, and western blotting analyses. A2E accumulation in the cytoplasm of ARPE-19 cells depending on the dose of A2E. Cell viability of ARPE-19 cells according to the dose of A2E and/or blue light intensity. The population of apoptotic or necrotic cells increased based on the A2E dose and blue light intensity. Codonopsis lanceolata dose-dependently prevented cell death which was induced by A2E and blue light. The antiapoptotic effect of that was caused by activating Keap1/Nrf2/HO-1 pathway, suppressing 4-HNE, and modulating Bcl-2 family proteins like increase of antiapoptotic proteins such as Bcl-2 and Bcl-XL and decrease of proapoptotic protein such as Bim. Based on these findings, 30 μM A2E and 20 mW/cm

Identifiants

DOI: 10.1038/s41598-024-67044-3 PMID: 39009704
pubmed: 39009704
doi: 10.1038/s41598-024-67044-3
pii: 10.1038/s41598-024-67044-3
doi:

Substances chimiques

NF-E2-Related Factor 2 0
Plant Extracts 0
NFE2L2 protein, human 0
Antioxidants 0
Kelch-Like ECH-Associated Protein 1 0
Aldehydes 0
4-hydroxy-2-nonenal K1CVM13F96
KEAP1 protein, human 0
Proto-Oncogene Proteins c-bcl-2 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

16322

Subventions

Organisme : the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT)
ID : 2022R1A5A8033794

Informations de copyright

© 2024. The Author(s).

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Auteurs

Soon-Young Lee (SY)

College of Korean Medicine, Dongshin University, Naju, 58245, Jeonnam, Korea.

Yeon-Kyoung Cho (YK)

College of Health and Welfare, Dongshin University, Naju, 58245, Jeonnam, Korea.

Chun-Sik Bae (CS)

College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Korea.

Gyeyeop Kim (G)

College of Health and Welfare, Dongshin University, Naju, 58245, Jeonnam, Korea.

Min-Jae Lee (MJ)

College of Veterinary Medicine, Kangwon National University, Chuncheon, 24341, Gangwon, Korea.

Seung-Sik Cho (SS)

Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan, 58554, Jeonnam, Korea. sscho@mokpo.ac.kr.
Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, 58579, Jeonnam, Korea. sscho@mokpo.ac.kr.

In-Chul Jeon (IC)

College of Health and Welfare, Dongshin University, Naju, 58245, Jeonnam, Korea. icjeon@dsu.ac.kr.

Dae-Hun Park (DH)

College of Korean Medicine, Dongshin University, Naju, 58245, Jeonnam, Korea. dhj1221@hanmail.net.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Campanulaceae › Codonopsis › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Maladies de l'oeil › Rétinopathies › Dégénérescence de la rétine › Dégénérescence maculaire › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique et à glissière à leucines › Facteur-2 apparenté à NF-E2 › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Préparations pharmaceutiques › Extraits de plantes › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Protéine-1 de type kelch associée à ECH › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Organes des sens › Oeil › Rétine › Épithélium pigmentaire de la rétine › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Oxidative and carbonyl stress induced AMD and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-bcl-2 › Oxidative and carbonyl stress induced AMD and...