Polar metabolomics using trichloroacetic acid extraction and porous graphitic carbon stationary phase.
HILIC
LC–MS
Polar metabolites
Porous graphitic carbon
Trichloroacetic acid
Untargeted metabolomics
Journal
Metabolomics : Official journal of the Metabolomic Society
ISSN: 1573-3890
Titre abrégé: Metabolomics
Pays: United States
ID NLM: 101274889
Informations de publication
Date de publication:
16 Jul 2024
16 Jul 2024
Historique:
received:
21
12
2023
accepted:
24
06
2024
medline:
17
7
2024
pubmed:
17
7
2024
entrez:
16
7
2024
Statut:
epublish
Résumé
Accurately identifying and quantifying polar metabolites using untargeted metabolomics has proven challenging in comparison to mid to non-polar metabolites. Hydrophilic interaction chromatography and gas chromatography-mass spectrometry are predominantly used to target polar metabolites. This study aims to demonstrate a simple one-step extraction combined with liquid chromatography-mass spectrometry (LC-MS) that reliably retains polar metabolites. The method involves a MilliQ + 10% trichloroacetic acid extraction from 6 healthy individuals serum, combined with porous graphitic carbon liquid chromatography-mass spectrometry (LC-MS). The coefficient of variation (CV) assessed retention reliability of polar metabolites with logP as low as - 9. QreSS (Quantification, Retention, and System Suitability) internal standards determined the method's consistency and recovery efficiency. The method demonstrated reliable retention (CV < 0.30) of polar metabolites within a logP range of - 9.1 to 5.6. QreSS internal standards confirmed consistent performance (CV < 0.16) and effective recovery (70-130%) of polar to mid-polar metabolites. Quality control dilution series demonstrated that ~ 80% of annotated metabolites could be accurately quantified (Pearson's correlation coefficient > 0.80) within their concentration range. Repeatability was demonstrated through clustering of repeated extractions from a single sample. This LC-MS method is better suited to covering the polar segment of the metabolome than current methods, offering a reliable and efficient approach for accurate quantification of polar metabolites in untargeted metabolomics.
Identifiants
pubmed: 39014104
doi: 10.1007/s11306-024-02146-7
pii: 10.1007/s11306-024-02146-7
doi:
Substances chimiques
Trichloroacetic Acid
5V2JDO056X
Graphite
7782-42-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
77Subventions
Organisme : High-Value Nutrition Ko Ngā Kai Whai Painga National Science Challenge
ID : HVN1917
Organisme : High-Value Nutrition Ko Ngā Kai Whai Painga National Science Challenge
ID : HVN1917
Informations de copyright
© 2024. The Author(s).
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