Potency and mechanism of p-glycoprotein chemosensitizers in rainbow trout (Oncorhynchus mykiss) hepatocytes.

Chemosensitizer Hepatocytes P-glycoprotein Rainbow trout

Journal

Fish physiology and biochemistry

ISSN: 1573-5168

Titre abrégé: Fish Physiol Biochem

Pays: Netherlands

ID NLM: 100955049

Informations de publication

Date de publication:
19 Jul 2024

Historique:

received: 31 08 2023

accepted: 02 07 2024

medline: 19 7 2024

pubmed: 19 7 2024

entrez: 18 7 2024

Statut: aheadofprint

Résumé

The membrane efflux transporter P-glycoprotein (P-gp, [ABCB1, MDR1]) exports a wide range of xenobiotic compounds, resulting in a continuous first line of defense against toxicant accumulation at basal expression levels, and contributing to the multixenobiotic resistance (MXR) phenotype at elevated expression levels. Relatively little information exists on P-gp inhibition in fish by chemosensitizers, compounds which lower toxicity thresholds for harmful P-gp substrates in complex mixtures. The effects of four known mammalian chemosensitizers (cyclosporin A [CsA], quinidine, valspodar [PSC833], and verapamil) on the P-gp-mediated transport of rhodamine 123 (R123) and cortisol in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes were examined. Competitive accumulation assays using 25 µM R123 or cortisol and varying concentrations of chemosensitizers (0-500 µM) were used. CsA, quinidine, and verapamil inhibited R123 export (IC50 values ± SE: 132 ± 60, 83.3 ± 27.2, and 43.2 ± 13.6 µM, respectively). CsA and valspodar inhibited cortisol export (IC50 values: 294 ± 106 and 92.2 ± 34.9 µM, respectively). In an ATP depletion assay, hepatocytes incubated with all four chemosensitizers resulted in lower free ATP concentrations, suggesting that they act via competitive inhibition. Chemosensitizers that inhibit MXR transporters are an important class of environmental pollutant, and these results show that rainbow trout transporters are inhibited by similar chemosensitizers (and mostly at similar concentrations) as seen in mammals and other fish species.

Identifiants

DOI: 10.1007/s10695-024-01376-9 PMID: 39026113

pubmed: 39026113

doi: 10.1007/s10695-024-01376-9

pii: 10.1007/s10695-024-01376-9

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Subventions

Organisme : Natural Sciences and Engineering Research Council of Canada

ID : R611305

Informations de copyright

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