Immunological assay using a solid-state pore with a low limit of detection.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 Jul 2024
19 Jul 2024
Historique:
received:
27
12
2023
accepted:
08
07
2024
medline:
20
7
2024
pubmed:
20
7
2024
entrez:
19
7
2024
Statut:
epublish
Résumé
Emerging infectious diseases, cancer, and other diseases are quickly tested mainly via immune reactions based on specific molecular recognition between antigens and antibodies. By changing the diameter of solid-state pores, biomolecules of various sizes can be rapidly detected at the single-molecule level. The combination of immunoreactions and solid-state pores paves the way for an efficient testing method with high specificity and sensitivity. The challenge in developing this method is achieving quantitative analysis using solid-state pores. Here, we demonstrate a method with a low limit of detection for testing tumor markers using a combination of immunoreactions and solid-state pore technology. Quantitative analysis of the mixing ratio of two and three beads with different diameters was achieved with an error rate of up to 4.7%. The hybrid solid-state pore and immunoreaction methods with prostate-specific antigen (PSA) and anti-PSA antibody-modified beads achieved a detection limit of 24.9 fM PSA in 30 min. The hybrid solid-state pore and immunoreaction enabled the rapid development of easy-to-use tests with lower limit of detection and greater throughput than commercially available immunoassay for point-of-care testing.
Identifiants
pubmed: 39030274
doi: 10.1038/s41598-024-67112-8
pii: 10.1038/s41598-024-67112-8
doi:
Substances chimiques
Prostate-Specific Antigen
EC 3.4.21.77
Biomarkers, Tumor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
16686Subventions
Organisme : Japan Agency for Medical Research and Development
ID : #JP21zf0127004
Informations de copyright
© 2024. The Author(s).
Références
Branton, D. et al. The potential and challenges of nanopore sequencing. Nat. Biotech. 26, 1146–1153 (2008).
doi: 10.1038/nbt.1495
Dekker, C. Solid-state nanopores. Nat. Nanotech. 2, 209–215 (2007).
doi: 10.1038/nnano.2007.27
Howorka, S. & Siwy, Z. Nanopore analytics: sensing of single molecules. Chem. Soc. Rev. 38, 2360–2384 (2009).
pubmed: 19623355
doi: 10.1039/b813796j
Li, J. et al. Ion-beam sculpting at nanometre length scales. Nature 412, 166–169 (2001).
pubmed: 11449268
doi: 10.1038/35084037
Storm, A. J., Chen, J. H., Ling, X. S., Zandbergen, H. W. & Dekker, C. Fabrication of solid-state nanopores with single-nanometre precision. Nat. Mater. 2, 537–540 (2003).
pubmed: 12858166
doi: 10.1038/nmat941
Merchant, C. A. et al. DNA translocation through graphene nanopores. Nano Lett. 10, 2915–2921 (2010).
pubmed: 20698604
doi: 10.1021/nl101046t
Schneider, G. F. et al. DNA translocation through graphene nanopores. Nano Lett. 10, 3163–3167 (2010).
pubmed: 20608744
doi: 10.1021/nl102069z
Feng, J. D. et al. Identification of single nucleotides in MoS
doi: 10.1038/nnano.2015.219
Fologea, D. et al. Detecting single stranded DNA with a solid state nanopore. Nano Lett. 5, 1905–1909 (2005).
pubmed: 16218707
pmcid: 2543124
doi: 10.1021/nl051199m
Li, J. L., Gershow, M., Stein, D., Brandin, E. & Golovchenko, J. A. DNA molecules and configurations in a solid-state nanopore microscope. Nat. Mater. 2, 611–615 (2003).
pubmed: 12942073
doi: 10.1038/nmat965
Wanunu, M. et al. Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors. Nat. Nanotech. 5, 807–814 (2010).
doi: 10.1038/nnano.2010.202
Zahid, O. K., Wang, F., Ruzicka, J. A., Taylor, E. W. & Hall, A. R. Sequence-specific recognition of microRNAs and other short nucleic acids with solid-state nanopores. Nano Lett. 16, 2033–2039 (2016).
pubmed: 26824296
pmcid: 5367926
doi: 10.1021/acs.nanolett.6b00001
Talaga, D. S. & Li, J. L. Single-molecule protein unfolding in solid state nanopores. J. Am. Chem. Soc. 131, 9287–9297 (2009).
pubmed: 19530678
pmcid: 2717167
doi: 10.1021/ja901088b
Kowalczyk, S. W., Hall, A. R. & Dekker, C. Detection of local protein structures along DNA using solid-state nanopores. Nano Lett. 10, 324–328 (2010).
pubmed: 19902919
doi: 10.1021/nl903631m
Plesa, C. et al. Fast Translocation of proteins through solid state nanopores. Nano Lett. 13, 658–663 (2013).
pubmed: 23343345
pmcid: 4151282
doi: 10.1021/nl3042678
Wei, R. S., Gatterdam, V., Wieneke, R., Tampe, R. & Rant, U. Stochastic sensing of proteins with receptor-modified solid-state nanopores. Nat. Nanotech. 7, 257–263 (2012).
doi: 10.1038/nnano.2012.24
McMullen, A., de Haan, H. W., Tang, J. X. & Stein, D. Stiff filamentous virus translocations through solid-state nanopores. Nat. Commun. 5, 4171 (2014).
pubmed: 24932700
doi: 10.1038/ncomms5171
Arima, A. et al. Identifying single viruses using biorecognition solid-state nanopores. J. Am. Chem. Soc. 140, 16834–16841 (2018).
pubmed: 30475615
doi: 10.1021/jacs.8b10854
Arima, A. et al. Selective detections of single-viruses using solid-state nanopores. Sci. Rep. 8, 16305 (2018).
pubmed: 30390013
pmcid: 6214978
doi: 10.1038/s41598-018-34665-4
Taniguchi, M. et al. Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection. Nat. Commun. 12, 3726 (2021).
pubmed: 34140500
pmcid: 8211865
doi: 10.1038/s41467-021-24001-2
Wu, H. W. et al. Translocation of rigid rod-shaped virus through various solid-state nanopores. Anal. Chem. 88, 2502–2510 (2016).
pubmed: 26790522
doi: 10.1021/acs.analchem.5b04905
Tsutsui, M. et al. Discriminating single-bacterial shape using low-aspect-ratio pores. Sci. Rep. 7, 17371 (2017).
pubmed: 29234023
pmcid: 5727063
doi: 10.1038/s41598-017-17443-6
Tsutsui, M. et al. Identification of individual bacterial cells through the intermolecular interactions with peptide-functionalized solid-state pores. Anal. Chem. 90, 1511–1515 (2018).
pubmed: 29350898
doi: 10.1021/acs.analchem.7b04950
Jia, C. P. et al. Nano-ELISA for highly sensitive protein detection. Biosens. Bioelectron. 24, 2836–2841 (2009).
pubmed: 19339168
doi: 10.1016/j.bios.2009.02.024
Dixit, C. K., Vashist, S. K., MacCraith, B. D. & O’Kennedy, R. Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays. Nat. Protoc. 6, 439–445 (2011).
pubmed: 21412272
doi: 10.1038/nprot.2011.304
Cheng, C. M. et al. Paper-based ELISA. Angew. Chem. Int. Ed Engl. 49, 4771–4774 (2010).
pubmed: 20512830
doi: 10.1002/anie.201001005
Han, A. et al. Label-free detection of single protein molecules and protein-protein interactions using synthetic nanopores. Anal. Chem. 80, 4651–4658 (2008).
pubmed: 18470996
doi: 10.1021/ac7025207
Ren, R. et al. Single-molecule binding assay using nanopores and dimeric NP conjugates. Adv. Mater. 33, 2103067 (2021).
doi: 10.1002/adma.202103067
Venkatesan, B. M. & Bashir, R. Nanopore sensors for nucleic acid analysis. Nat. Nanotechnol. 6, 615–624 (2011).
pubmed: 21926981
doi: 10.1038/nnano.2011.129
Daiguji, H. Ion transport in nanofluidic channels. Chem. Soc. Rev. 39, 901–911 (2010).
pubmed: 20179813
doi: 10.1039/B820556F
Wen, C. Y. & Zhang, S. L. Fundamentals and potentials of solid-state nanopores: A review. J. Phys. D Appl. Phys. 54, 023001 (2021).
doi: 10.1088/1361-6463/ababce
Lee, K. et al. Recent progress in solid-state nanopores. Adv. Mater. 30, 1704680 (2018).
doi: 10.1002/adma.201704680
Fragasso, A., Schmid, S. & Dekker, C. Comparing current noise in biological and solid-state nanopores. ACS Nano 14, 1338–1349 (2020).
pubmed: 32049492
pmcid: 7045697
doi: 10.1021/acsnano.9b09353
Rosenstein, J. K., Wanunu, M., Merchant, C. A., Drndic, M. & Shepard, K. L. Integrated nanopore sensing platform with sub-microsecond temporal resolution. Nat. Methods 9, 487–492 (2012).
pubmed: 22426489
doi: 10.1038/nmeth.1932
Fologea, D., Uplinger, J., Thomas, B., McNabb, D. S. & Li, J. L. Slowing DNA translocation in a solid-state nanopore. Nano Lett. 5, 1734–1737 (2005).
pubmed: 16159215
pmcid: 3037730
doi: 10.1021/nl051063o
Kowalczyk, S. W., Wells, D. B., Aksimentiev, A. & Dekker, C. Slowing down DNA translocation through a nanopore in lithium chloride. Nano Lett. 12, 1038–1044 (2012).
pubmed: 22229707
pmcid: 3349906
doi: 10.1021/nl204273h
Lu, B. et al. Pressure-controlled motion of single polymers through solid-state nanopores. Nano Lett. 13, 3048–3052 (2013).
pubmed: 23802688
pmcid: 3864131
doi: 10.1021/nl402052v
Keyser, U. F. et al. Direct force measurements on DNA in a solid-state nanopore. Nat. Phys. 2, 473–477 (2006).
doi: 10.1038/nphys344
Davenport, M. et al. The role of pore geometry in single nanoparticle detection. ACS Nano 6, 8366–8380 (2012).
pubmed: 22913710
doi: 10.1021/nn303126n
Taniguchi, M., Takei, H., Tomiyasu, K., Sakamoto, O. & Naono, N. Sensing the performance of artificially intelligent nanopores developed by integrating solid-state nanopores with machine learning methods. J. Phys. Chem. C 126, 12197–12209 (2022).
doi: 10.1021/acs.jpcc.2c02674
Tsutsui, M. et al. Particle trajectory-dependent ionic current blockade in low-aspect-ratio pores. AS Nano 10, 803–809 (2016).
doi: 10.1021/acsnano.5b05906
Qin, Z. P., Zhe, J. A. & Wang, G. X. Effects of particle’s off-axis position, shape, orientation and entry position on resistance changes of micro coulter counting devices. Meas. Sci. Technol. 22, 045804 (2011).
doi: 10.1088/0957-0233/22/4/045804
Saleh, O. A. & Sohn, L. L. Correcting off-axis effects in an on-chip resistive-pulse analyzer. Rev. Sci. Instrum. 73, 4396–4398 (2002).
doi: 10.1063/1.1519932
Prensner, J. R., Rubin, M. A., Wei, J. T. & Chinnaiyan, A. M. Beyond PSA: The next generation of prostate cancer biomarkers. Sci. Transl. Med. 4, 27rv3 (2012).
doi: 10.1126/scitranslmed.3003180
Duffy, M. J. Biomarkers for prostate cancer: Prostate-specific antigen and beyond. Clin. Chem. Lab. Med. 58, 326–339 (2020).
pubmed: 31714881
doi: 10.1515/cclm-2019-0693
Kim, S. H. et al. Large-scale femtoliter droplet array for digital counting of single biomolecules. Lab Chip 12, 4986–4991 (2012).
pubmed: 22961607
doi: 10.1039/c2lc40632b
Rissin, D. M. et al. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations. Nat. Biotech. 28, 595–599 (2010).
doi: 10.1038/nbt.1641
Platt, M., Willmott, G. R. & Lee, G. U. Resistive pulse sensing of analyte-induced multicomponent rod aggregation using tunable pores. Small 8, 2436–2444 (2012).
pubmed: 22570187
doi: 10.1002/smll.201200058
Chuah, K. et al. Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples. Nat. Commun. 10, 2109 (2019).
pubmed: 31068594
pmcid: 6506515
doi: 10.1038/s41467-019-10147-7
Wu, Y. F., Yao, Y., Cheong, S., Tilley, R. D. & Gooding, J. J. Selectively detecting attomolar concentrations of proteins using gold lined nanopores in a nanopore blockade sensor. Chem. Sci. 11, 12570–12579 (2020).
pubmed: 34094456
pmcid: 8163308
doi: 10.1039/D0SC04552G
Liu, A., Zhao, F., Zhao, Y., Shangguan, L. & Liu, S. A portable chemiluminescence imaging immunoassay for simultaneous detection of different isoforms of prostate specific antigen in serum. Biosens. Bioelectron. 81, 97–102 (2016).
pubmed: 26922048
doi: 10.1016/j.bios.2016.02.049