The potential role of collagen type VII in breast cancer proliferation.
Breast cancer
Collagen type VII
Extracellular matrix
Mesenchymal stem cell
Journal
Cancer cell international
ISSN: 1475-2867
Titre abrégé: Cancer Cell Int
Pays: England
ID NLM: 101139795
Informations de publication
Date de publication:
20 Jul 2024
20 Jul 2024
Historique:
received:
22
04
2024
accepted:
12
07
2024
medline:
20
7
2024
pubmed:
20
7
2024
entrez:
19
7
2024
Statut:
epublish
Résumé
Breast cancer is the most common cancer in women. Cancer cells can persist in a prolonged dormant state for years without any clinical evidence of disease creating an urgent need to better understand the molecular mechanisms leading to relapse. This study aimed to identify extracellular matrix (ECM) components associated with hypoxia-induced breast cancer dormancy. The effects of selected ECM proteins on breast cancer cell proliferation were analyzed, along with their correlation with established prognostic markers in human breast cancer tissue. Screening of extracellular matrix proteins was performed in hypoxia-induced dormant MCF-7 breast cancer cells. Proliferation of MCF-7 cells in vitro was subsequently determined in the presence of recombinant ColVII. Adipose tissue-derived mesenchymal stem cells (AdMSCs) subpopulation overexpressing ColVII were indirectly isolated by ColVII receptor integrin-α6 specific antibodies. AdMSCs- MCF-7 3D spheroid cultures were generated to model solid tumour conditions. In addition, the association between ColVII and various prognostic markers was evaluated in clinical samples of human breast cancer tissue. Dormant MCF-7 cells showed an elevated expression of ColVII while MCF-7 cells cultured on ColVII exhibited reduced proliferation in vitro. In AdMSCs-MCF-7 3D spheroids, a reduced proliferation of MCF-7 cells was observed in Int-α6 ColVII is associated with reduced proliferation of breast cancer cells in vitro. ColVII is strongly expressed in myoepithelial cells and in breast cancer tissue the high ColVII expression correlates with several well-known positive prognostic markers, highlighting its potential as a prognostic marker in breast cancer.
Sections du résumé
BACKGROUND
BACKGROUND
Breast cancer is the most common cancer in women. Cancer cells can persist in a prolonged dormant state for years without any clinical evidence of disease creating an urgent need to better understand the molecular mechanisms leading to relapse. This study aimed to identify extracellular matrix (ECM) components associated with hypoxia-induced breast cancer dormancy. The effects of selected ECM proteins on breast cancer cell proliferation were analyzed, along with their correlation with established prognostic markers in human breast cancer tissue.
MATERIALS AND METHODS
METHODS
Screening of extracellular matrix proteins was performed in hypoxia-induced dormant MCF-7 breast cancer cells. Proliferation of MCF-7 cells in vitro was subsequently determined in the presence of recombinant ColVII. Adipose tissue-derived mesenchymal stem cells (AdMSCs) subpopulation overexpressing ColVII were indirectly isolated by ColVII receptor integrin-α6 specific antibodies. AdMSCs- MCF-7 3D spheroid cultures were generated to model solid tumour conditions. In addition, the association between ColVII and various prognostic markers was evaluated in clinical samples of human breast cancer tissue.
RESULTS
RESULTS
Dormant MCF-7 cells showed an elevated expression of ColVII while MCF-7 cells cultured on ColVII exhibited reduced proliferation in vitro. In AdMSCs-MCF-7 3D spheroids, a reduced proliferation of MCF-7 cells was observed in Int-α6
CONCLUSION
CONCLUSIONS
ColVII is associated with reduced proliferation of breast cancer cells in vitro. ColVII is strongly expressed in myoepithelial cells and in breast cancer tissue the high ColVII expression correlates with several well-known positive prognostic markers, highlighting its potential as a prognostic marker in breast cancer.
Identifiants
pubmed: 39030607
doi: 10.1186/s12935-024-03449-4
pii: 10.1186/s12935-024-03449-4
doi:
Types de publication
Journal Article
Langues
eng
Pagination
254Informations de copyright
© 2024. The Author(s).
Références
Sung H, Global Cancer Statistics. 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries, CA Cancer J Clin, 2021;71(3):209–249. https://doi.org/10.3322/CAAC.21660 .
Valachis A, et al. Use of classifiers to optimise the identification and characterisation of metastatic breast cancer in a nationwide administrative registry. Acta Oncol (Madr). 2021;60(12):1604–10. https://doi.org/10.1080/0284186X.2021.1979645 .
doi: 10.1080/0284186X.2021.1979645
Ono M, et al. Exosomes from bone marrow mesenchymal stem cells contain a microRNA that promotes dormancy in metastatic breast cancer cells. Sci Signal. 2014;7(332):ra63–63. https://doi.org/10.1126/scisignal.2005231 .
doi: 10.1126/scisignal.2005231
pubmed: 24985346
Seltzer S, Corrigan M, O’Reilly S. The clinicomolecular landscape of de novo versus relapsed stage IV metastatic breast cancer. Exp Mol Pathol. Jun. 2020;114:104404. https://doi.org/10.1016/J.YEXMP.2020.104404 .
Bütepage G, Carlqvist P, Jacob J, Toft Hornemann A, Vertuani S. Overall survival of individuals with metastatic cancer in Sweden: a nationwide study. BMC Public Health. Dec. 2022;22(1):1–9. https://doi.org/10.1186/S12889-022-14255-W/TABLES/3 .
Polyak K, Kalluri R. The role of the microenvironment in mammary gland development and cancer., Cold Spring Harbor perspectives in biology. 11 Cold Spring Harb Perspect Biol. 2010;2. https://doi.org/10.1101/cshperspect.a003244 .
Ritter A, Kreis NN, Hoock SC, Solbach C, Louwen F, Yuan J. Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells, Obesity and the Tumor Microenvironment of Breast Cancer, Cancers, 2022;14(16):MDPI. https://doi.org/10.3390/cancers14163908 .
Alexander J, Cukierman E. Cancer associated fibroblast: Mediators of tumorigenesis, Matrix Biol, 2020;91–92:19–34. https://doi.org/10.1016/J.MATBIO.2020.05.004 .
Miyazaki Y et al. Feb., Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model, Scientific Reports 2021 11:1. 2021;11(1):1–12. https://doi.org/10.1038/s41598-021-84058-3 .
Insua-Rodríguez J, Oskarsson T. The extracellular matrix in breast cancer. Adv Drug Deliv Rev. 2016;97:41–55. https://doi.org/10.1016/J.ADDR.2015.12.017 .
Egeblad M, Rasch MG, Weaver VM. Dynamic interplay between the collagen scaffold and tumor evolution. Curr Opin Cell Biol. 2010;22(5):697. https://doi.org/10.1016/J.CEB.2010.08.015 .
Lepucki A, Orlińska K, Mielczarek-Palacz A, Kabut J, Olczyk P, Komosińska-Vassev K. The Role of Extracellular Matrix Proteins in Breast Cancer, Journal of Clinical Medicine. 2022;11(5):MDPI. https://doi.org/10.3390/jcm11051250 .
Gelse K, Pöschl E, Aigner T. Collagens—structure, function, and biosynthesis, Adv Drug Deliv Rev, 2002;55(12):1531–1546. https://doi.org/10.1016/J.ADDR.2003.08.002 .
Marinkovich MP, Tang JY, Elsevier BV. 2019;1221–6. https://doi.org/10.1016/j.jid.2018.11.036 .
Hong Y, Kim N, Li C, Jeong E, Yoon S. Patient sample-oriented analysis of gene expression highlights extracellular signatures in breast cancer progression. Biochem Biophys Res Commun. May 2017;487(2):307–12. https://doi.org/10.1016/J.BBRC.2017.04.055 .
Lauvrud AT et al. Oct., Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential, J Plast Reconstr Aesthet Surg. 2021;74(10):2759–2767. https://doi.org/10.1016/J.BJPS.2021.03.025 .
Lee HR, Leslie F, Azarin SM. A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl2. J Biol Eng. Aug. 2018;12(1). https://doi.org/10.1186/s13036-018-0106-7 .
Liebert M, Washington R, Stein J, Wedemeyer G, Grossman HB. Expression of the VLA beta 1 integrin family in bladder cancer., Am J Pathol. 1994;144(5):1016. Accessed: Nov. 10, 2023. [Online]. Available: /pmc/articles/PMC1887346/?report = abstract .
Bankhead P, et al. QuPath: open source software for digital pathology image analysis. Sci Rep. Dec. 2017;7(1). https://doi.org/10.1038/S41598-017-17204-5 .
Hubbi ME, Semenza GL. Regulation of cell proliferation by hypoxia-inducible factors, Am J Physiol Cell Physiol. 2015;309(12):C775. https://doi.org/10.1152/AJPCELL.00279.2015 .
Phan TG, Croucher PI. The dormant cancer cell life cycle, Nature Reviews Cancer, 2020;20(7). Nature Research, pp. 398–411. https://doi.org/10.1038/s41568-020-0263-0 .
Karlsson M, et al. A single-cell type transcriptomics map of human tissues. Sci Adv. 2021;7(31). https://doi.org/10.1126/SCIADV.ABH2169 .
Pérez-Díaz S et al. Sep., Obesity impairs skeletal muscle repair through NID-1 mediated extracellular matrix remodeling by mesenchymal progenitors, Matrix Biol. 2022;112:90–115. https://doi.org/10.1016/J.MATBIO.2022.08.006 .
Mortensen JH, Karsdal MA, Collagen TVII. Biochemistry of Collagens, Laminins and Elastin: Structure, Function and Biomarkers. 2016, pp. 57–60. https://doi.org/10.1016/B978-0-12-809847-9.00007-6 .
Kleiser S, Nyström A. Interplay between Cell-Surface Receptors and Extracellular Matrix in Skin, Biomolecules 2020, Vol. 10, Page 1170, 2020;10(8):1170. https://doi.org/10.3390/BIOM10081170 .
Commons JD, Brittingham R, Uitto J, Fertala A. High-Affinity Binding of the NC1 Domain of Collagen VII to Laminin 5 and Collagen IV, 2006, Accessed: Feb. 20, 2024. [Online]. Available: http://jdc.jefferson.edu/dcbfp/2 .
Natali PG, Nicotra MR, Botti C, Mottolese M, Bigotti A, Segatto O. Changes in expression of alpha 6/beta 4 integrin heterodimer in primary and metastatic breast cancer., Br J Cancer, 1992;66(2):318–322. https://doi.org/10.1038/BJC.1992.263 .
Karlsson M, et al. A single–cell type transcriptomics map of human tissues. Sci Adv. 2021;7(31). https://doi.org/10.1126/SCIADV.ABH2169/ . SUPPL_FILE/SCIADV.ABH2169_SM.PDF.
Deugnier MA, Teulière J, Faraldo MM, Thiery JP, Glukhova MA. The importance of being a myoepithelial cell. Breast Cancer Res. 2002;4:224–30. https://doi.org/10.1186/bcr459 . no. 6.
doi: 10.1186/bcr459
pubmed: 12473168
pmcid: 137933
Duivenvoorden HM, Brockwell NK, Nowell CJ, Simpson KJ, Parker BS. High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins. Sci Data. Dec. 2021;8(1). https://doi.org/10.1038/S41597-021-00924-9 .
Fine JD. Inherited epidermolysis bullosa. Orphanet J Rare Dis. 2010;5(1). https://doi.org/10.1186/1750-1172-5-12 .
Martins VL et al. Jun., Increased invasive behaviour in cutaneous squamous cell carcinoma with loss of basement-membrane type VII collagen, J Cell Sci. 2009;122(11):1788–1799. https://doi.org/10.1242/jcs.042895 .
Oh SE, et al. Prognostic value of highly expressed type VII collagen (COL7A1) in patients with gastric Cancer. Pathol Oncol Res. Aug. 2021;27. https://doi.org/10.3389/pore.2021.1609860 .
Kita Y, et al. Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma. Eur J Surg Oncol. Jan. 2009;35(1):52–8. https://doi.org/10.1016/J.EJSO.2008.01.025 .