The Bifidobacterium adolescentis BAD_1527 gene encodes GH43_22 α-L-arabinofuranosidase of AXH-m type.

Bifidobacterium adolescentis Arabinan Arabinoxylan Positional specificity Substrate specificity α-L-Arabinofuranosidase

Journal

AMB Express

ISSN: 2191-0855

Titre abrégé: AMB Express

Pays: Germany

ID NLM: 101561785

Informations de publication

Date de publication:
20 Jul 2024

Historique:

received: 21 04 2024

accepted: 25 06 2024

medline: 21 7 2024

pubmed: 21 7 2024

entrez: 20 7 2024

Statut: epublish

Résumé

Bifidobacterium adolescentis gene BAD_1527 has previously been suggested to code for a β-xylosidase (Kobayashi et al., Mar Drugs 18:174, 2020). Our detailed investigation of the substrate specificity of the GH43_22 protein using a wide spectrum of natural and artificial substrates showed that the enzyme hydrolyzed neither linear xylooligosaccharides nor glucuronoxylan. Xylose was released only from the artificial 4-nitrophenyl β-D-xylopyranoside (1.58 mU/mg). The corresponding α-L-arabinofuranoside was by three orders of magnitude better substrate (2.17 U/mg). Arabinose was the only monosaccharide liberated from arabinoxylan and α-1,3- or α-1,2-singly arabinosylated xylooligosaccharides. Moreover, the enzyme efficiently debranched sugar beet arabinan and singly arabinosylated α-1,5-L-arabinooligosaccharides, although short linear α-1,5-L-arabinooligosaccharides were also slowly degraded. On the other hand, debranched arabinan, arabinogalactan as well as 2,3-doubly arabinosylated main chain residues of arabinan and arabinoxylan did not serve as substrates. Thus, the enzyme encoded by the BAD_1527 gene is a typical α-L-arabinofuranosidase of AXH-m specificity.

Identifiants

DOI: 10.1186/s13568-024-01738-9 PMID: 39033088

pubmed: 39033088

doi: 10.1186/s13568-024-01738-9

pii: 10.1186/s13568-024-01738-9

doi:

Types de publication

Journal Article

Langues

eng

Pagination

Subventions

Organisme : Agentúra na Podporu Výskumu a Vývoja

ID : APVV-20-0591

Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV

ID : 2/0171/22

Informations de copyright

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