Platelet-derived biomaterial controls aspergillus fumigatus keratitis by decreasing fungal burden: an in vivo study.
Aspergillus Fumigatus
Fungal keratitis
New treatment
Platelets-derived biomaterials
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
20 Jul 2024
20 Jul 2024
Historique:
received:
14
05
2024
accepted:
15
07
2024
revised:
30
06
2024
medline:
21
7
2024
pubmed:
21
7
2024
entrez:
20
7
2024
Statut:
epublish
Résumé
Fungal keratitis is a severe corneal infection characterized by suppurative and ulcerative lesions. Aspergillus fumigatus is a common cause of fungal keratitis. Antifungal drugs, such as natamycin, are currently the first-line treatment for fungal keratitis, but their ineffectiveness leads to blindness and perforation. Additionally, the development of fungal resistance makes treating fungal keratitis significantly more challenging. The present study used platelet-derived biomaterial (PDB) to manage A. fumigatus keratitis in the animal model. Freezing and thawing processes were used to prepare PDB, and then A. fumigatus keratitis was induced in the mice. Topical administration of PDB, natamycin, and plasma was performed; quantitative real-time PCR (qPCR) and histopathologic examination (HE) were used to assess the inhibitory effect of the mentioned compounds against fungal keratitis. The qPCR results showed that PDB significantly decreased the count of A. fumigatus compared to the control group (P-value ≤ 5). Natamycin also remarkably reduced the count of fungi in comparison to the untreated animal, but its inhibitory effect was not better than PDB (P-value > 5). The findings of HE also demonstrated that treatment with PDB and natamycin decreased the fungal loads in the corneal tissue. However, plasma did not show a significant inhibitory effect against A. fumigatus. PDB is intrinsically safe and free of any infections or allergic responses; additionally, this compound has a potential role in decreasing the burden of A. fumigatus and treating fungal keratitis. Therefore, scientists should consider PDB an applicable approach to managing fungal keratitis and an alternative to conventional antifungal agents.
Identifiants
pubmed: 39033220
doi: 10.1007/s00203-024-04084-3
pii: 10.1007/s00203-024-04084-3
doi:
Substances chimiques
Antifungal Agents
0
Biocompatible Materials
0
Natamycin
8O0C852CPO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
358Subventions
Organisme : Khomein University of Medical Sciences
ID : 4556
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Al-Hatmi AMS et al (2019) Epidemiology of aspergillus species causing keratitis in Mexico. Mycoses 62:144–151. https://doi.org/10.1111/myc.12855
doi: 10.1111/myc.12855
pubmed: 30256460
Alio JL, Pastor S, Ruiz-Colecha J, Rodriguez A, Artola A (2007) Treatment of ocular surface syndrome after LASIK with autologous platelet-rich plasma. J Refract Surg 23:617–619. https://doi.org/10.3928/1081-597x-20070601-13
doi: 10.3928/1081-597x-20070601-13
pubmed: 17598582
Anitua E et al (2022) Progress in the use of plasma rich in growth factors in ophthalmology: from ocular surface to ocular fundus. Expert Opin Biol Ther 22:31–45. https://doi.org/10.1080/14712598.2021.1945030
doi: 10.1080/14712598.2021.1945030
pubmed: 34275392
Ansari Z, Miller D, Galor A (2013) Current thoughts in fungal keratitis: diagnosis and treatment. Curr Fungal Infect Rep 7:209–218. https://doi.org/10.1007/s12281-013-0150-110.1007/s12281-013-0150-1
doi: 10.1007/s12281-013-0150-1
pubmed: 24040467
pmcid: 3768010
Azadi S et al (2024) Antifungal activity of Fe(3)O(4)@SiO(2)/Schiff-base/Cu(II) magnetic nanoparticles against pathogenic Candida species. Sci Rep 14:5855. https://doi.org/10.1038/s41598-024-56512-5
doi: 10.1038/s41598-024-56512-5
pubmed: 38467729
pmcid: 10928175
Borzini P, Mazzucco I (2007) Platelet-rich plasma (PRP) and platelet derivatives for topical therapy. What is true from the biologic view point? ISBT Sci Ser 2:272–281
doi: 10.1111/j.1751-2824.2007.00085.x
Costa C, Vidaud D, Olivi M, Bart-Delabesse E, Vidaud M, Bretagne S (2001) Development of two real-time quantitative TaqMan PCR assays to detect circulating aspergillus fumigatus DNA in serum. J Microbiol Methods 44:263–269. https://doi.org/10.1016/s0167-7012(01)00212-3
doi: 10.1016/s0167-7012(01)00212-3
pubmed: 11240049
Durand ML (2017) Bacterial and fungal endophthalmitis. Clin Microbiol Rev 30:597–613. https://doi.org/10.1128/cmr.00113-16
doi: 10.1128/cmr.00113-16
pubmed: 28356323
pmcid: 5475221
Gopinathan U, Garg P, Fernandes M, Sharma S, Athmanathan S, Rao GN (2002) The epidemiological features and laboratory results of fungal keratitis: a 10-year review at a referral eye care center in South India. Cornea 21:555–559. https://doi.org/10.1097/00003226-200208000-00004
doi: 10.1097/00003226-200208000-00004
pubmed: 12131029
Jangjou A et al (2022) Time to Conquer Fungal Infectious diseases: employing nanoparticles as powerful and versatile antifungal nanosystems against a wide Variety of Fungal species. Sustainability 14:12942
doi: 10.3390/su141912942
Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 85:638–646
Nadelmann JB, Bunya VY, Ying GS, Hua P, Massaro-Giordano M (2022) Effect of autologous platelet-rich plasma drops in the treatment of Ocular Surface Disease. Clin Ophthalmol 16:4207–4213. https://doi.org/10.2147/opth.s391536
doi: 10.2147/opth.s391536
pubmed: 36544899
pmcid: 9762402
Najafi E et al (2019) Application of platelet-rich plasma and platelet lysate in the treatment of experimental lymphocutaneous sporotrichosi. Curr Med Mycol 5:7–12. https://doi.org/10.18502/cmm.5.3.1740
doi: 10.18502/cmm.5.3.1740
pubmed: 31850390
pmcid: 6910710
Okumura Y et al (2022) Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing. Br J Ophthalmol. https://doi.org/10.1136/bjo-2022-322068
doi: 10.1136/bjo-2022-322068
Ozcelik U et al (2016) Effect of topical platelet-rich plasma on burn Healing after partial-thickness burn Injury. Med Sci Monit 22:1903–1909. https://doi.org/10.12659/msm.895395
doi: 10.12659/msm.895395
pubmed: 27262706
pmcid: 4913826
Pallua N, Wolter T, Markowicz M (2010) Platelet-rich plasma in burns. Burns 36:4–8
doi: 10.1016/j.burns.2009.05.002
pubmed: 19541423
Qiu WY et al (2005) Fungal spectrum identified by a new slide culture and in vitro drug susceptibility using Etest in fungal keratitis. Curr Eye Res 30:1113–1120. https://doi.org/10.1080/02713680500423671
doi: 10.1080/02713680500423671
pubmed: 16354625
Qiu S et al (2015) Natamycin in the treatment of fungal keratitis: a systematic review and Meta-analysis. Int J Ophthalmol 8:597–602. https://doi.org/10.3980/j.issn.2222-3959.2015.03.29
doi: 10.3980/j.issn.2222-3959.2015.03.29
pubmed: 26086015
pmcid: 4458670
Ratitong B, Pearlman E (2021) Pathogenic aspergillus and Fusarium as important causes of blinding corneal infections - the role of neutrophils in fungal killing, tissue damage and cytokine production. Curr Opin Microbiol 63:195–203. https://doi.org/10.1016/j.mib.2021.07.018
doi: 10.1016/j.mib.2021.07.018
pubmed: 34419783
pmcid: 10492570
Shariati A, Moradabadi A, Azimi T, Ghaznavi-Rad E (2020) Wound healing properties and antimicrobial activity of platelet-derived biomaterials. Sci Rep 10:1032. https://doi.org/10.1038/s41598-020-57559-w
doi: 10.1038/s41598-020-57559-w
pubmed: 31974417
pmcid: 6978467
Shariati A, Moradabadi A, Ghaznavi-Rad E, Dadmanesh M, Komijani M, Nojoomi F (2021a) Investigation into antibacterial and wound healing properties of platelets lysate against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections. Ann Clin Microbiol Antimicrob 20:40 https://doi.org/10.1186/s12941-021-00442-x
Shariati A et al (2021b) Association between colorectal cancer and Fusobacterium nucleatum and Bacteroides fragilis bacteria in Iranian patients: a preliminary study. Infect Agents Cancer 16:41. https://doi.org/10.1186/s13027-021-00381-4
doi: 10.1186/s13027-021-00381-4
Shariati A, Didehdar M, Razavi S, Heidary M, Soroush F, Chegini Z (2022) Natural compounds: a Hopeful Promise as an Antibiofilm Agent against Candida Species. Front Pharmacol 13:917787. https://doi.org/10.3389/fphar.2022.917787
doi: 10.3389/fphar.2022.917787
pubmed: 35899117
pmcid: 9309813
van Gils JM, Zwaginga JJ, Hordijk PL (2009) Molecular and functional interactions among monocytes, platelets, and endothelial cells and their relevance for cardiovascular diseases. J Leukoc Biol 85:195–204. https://doi.org/10.1189/jlb.0708400
doi: 10.1189/jlb.0708400
pubmed: 18948548
Vanathi M, Naik R, Sidhu N, Ahmed NH, Gupta N, Tandon R (2022) Evaluation of antifungal susceptibility and clinical characteristics in fungal keratitis in a tertiary care center in North India. Indian J Ophthalmol 70:4270–4283. https://doi.org/10.4103/ijo.IJO_855_22
doi: 10.4103/ijo.IJO_855_22
pubmed: 36453329
pmcid: 9940598
Viljoen KS, Dakshinamurthy A, Goldberg P, Blackburn JM (2015) Quantitative profiling of colorectal cancer-associated bacteria reveals associations between fusobacterium spp., enterotoxigenic Bacteroides fragilis (ETBF) and clinicopathological features of colorectal cancer. PLoS ONE 10:e0119462
doi: 10.1371/journal.pone.0119462
pubmed: 25751261
pmcid: 4353626
Wang L et al (2023) Thymol ameliorates aspergillus fumigatus keratitis by downregulating the TLR4/ MyD88/ NF-kB/ IL-1β Signal expression and reducing necroptosis and Pyroptosis. J Microbiol Biotechnol 33:43–50. https://doi.org/10.4014/jmb.2207.07017
doi: 10.4014/jmb.2207.07017
pubmed: 36517045
Wu TE, Chen CJ, Hu CC, Cheng CK (2015) Easy-to-prepare autologous platelet-rich plasma in the treatment of refractory corneal ulcers. Taiwan J Ophthalmol 5:132–135. https://doi.org/10.1016/j.tjo.2014.09.001
doi: 10.1016/j.tjo.2014.09.001
pubmed: 29018685
Yi W et al (2023) Benzyl isothiocyanate improves the prognosis of aspergillus fumigatus keratitis by reducing fungal load and inhibiting mincle signal pathway. Front Microbiol 14:1119568. https://doi.org/10.3389/fmicb.2023.1119568
doi: 10.3389/fmicb.2023.1119568
pubmed: 36876115
pmcid: 9978348
Yin M et al (2019) Expression and role of calcitonin gene-related peptide in mouse aspergillus fumigatus keratitis. Int J Ophthalmol 12:697–704. https://doi.org/10.18240/ijo.2019.05.01
doi: 10.18240/ijo.2019.05.01
pubmed: 31131225
pmcid: 6520267
Yin M et al (2023) Pseudolaric Acid B ameliorates fungal keratitis progression by suppressing inflammation and reducing fungal load. ACS Infect Dis. https://doi.org/10.1021/acsinfecdis.2c00536
doi: 10.1021/acsinfecdis.2c00536
pubmed: 37141176
Zhan L et al (2020) Honokiol reduces fungal load, toll-like Receptor-2, and Inflammatory cytokines in Aspergillus Fumigatus Keratitis. Investig Ophthalmol Vis Sci 61:48–48. https://doi.org/10.1167/iovs.61.4.48
doi: 10.1167/iovs.61.4.48
Zhan L et al (2023) Honokiol reduces fungal burden and ameliorate inflammation lesions of aspergillus fumigatus keratitis via Dectin-2 down-regulation. Int Immunopharmacol 118:109849. https://doi.org/10.1016/j.intimp.2023.109849
doi: 10.1016/j.intimp.2023.109849
pubmed: 36933490
Zhao W et al (2018) Fenretinide inhibits Neutrophil recruitment and IL-1β production in Aspergillus Fumigatus Keratitis. Cornea 37:1579–1585. https://doi.org/10.1097/ico.0000000000001756
doi: 10.1097/ico.0000000000001756
pubmed: 30211745
pmcid: 6221409