Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway.

Food allergy IL-10 TERT dendritic cell immunotherapy

Journal

Immunologic research
ISSN: 1559-0755
Titre abrégé: Immunol Res
Pays: United States
ID NLM: 8611087

Informations de publication

Date de publication:
22 Jul 2024
Historique:
received: 23 03 2024
accepted: 03 06 2024
medline: 22 7 2024
pubmed: 22 7 2024
entrez: 21 7 2024
Statut: aheadofprint

Résumé

The cause of food allergy (FA) is still a mystery. Telomerases are involved in the regulation of immune responses. This study aims to gain an understanding of the contribution of telomerase reverse transcriptase (TERT) to the pathogenesis of FA. A murine FA model was established with ovalbumin as the specific antigen. The role of TERT in regulating dendritic cell (DC) immune tolerogenic functions was evaluated in this murine model. We observed that the Tert promoter was at demethylation status and the Tert expression was elevated in DCs of FA mice. The Tert expression in DCs had a positive correlation with the FA response. TERT prevented the induction of Il10 expression in DCs. The immune tolerogenic functions of DCs were diminished by TERT. The immune tolerogenic functions of DC were restored by CpG by boosting the Tert promoter methylation. Administration of CpG promoted the therapeutic effects of allergen specific immunotherapy in FA mice. Low levels of Il10 expression and high levels of Tert expression were observed in intestinal DCs of FA mice. CpG exposure restored the expression of Il10 and increased the therapeutic benefits of allergen-specific immunotherapy.

Sections du résumé

BACKGROUND BACKGROUND
The cause of food allergy (FA) is still a mystery. Telomerases are involved in the regulation of immune responses. This study aims to gain an understanding of the contribution of telomerase reverse transcriptase (TERT) to the pathogenesis of FA.
METHODS METHODS
A murine FA model was established with ovalbumin as the specific antigen. The role of TERT in regulating dendritic cell (DC) immune tolerogenic functions was evaluated in this murine model.
RESULTS RESULTS
We observed that the Tert promoter was at demethylation status and the Tert expression was elevated in DCs of FA mice. The Tert expression in DCs had a positive correlation with the FA response. TERT prevented the induction of Il10 expression in DCs. The immune tolerogenic functions of DCs were diminished by TERT. The immune tolerogenic functions of DC were restored by CpG by boosting the Tert promoter methylation. Administration of CpG promoted the therapeutic effects of allergen specific immunotherapy in FA mice.
CONCLUSIONS CONCLUSIONS
Low levels of Il10 expression and high levels of Tert expression were observed in intestinal DCs of FA mice. CpG exposure restored the expression of Il10 and increased the therapeutic benefits of allergen-specific immunotherapy.

Identifiants

DOI: 10.1007/s12026-024-09504-6 PMID: 39034374
pubmed: 39034374
doi: 10.1007/s12026-024-09504-6
pii: 10.1007/s12026-024-09504-6
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Haotao Zeng (H)

Department of Allergy, Longgang ENT Hospital & Shenzhen ENT Institute, Shenzhen, China.

Lingzhi Xu (L)

Department of Immunology, School of Basic Medical Sciences, Weifang Medical University, Weifang, China.

Jiangqi Liu (J)

Department of Allergy, Longgang ENT Hospital & Shenzhen ENT Institute, Shenzhen, China.

Lihua Mo (L)

State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen University and Institute of Allergy & Immunology of Shenzhen University, Room A7-509 at Lihu Campus of Shenzhen University. 1066 Xueyuan Blvd, Shenzhen, 518055, China.
Department of General Practice Medicine, Third Affiliated Hospital, Shenzhen University, Shenzhen, China.

Minyao Li (M)

State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen University and Institute of Allergy & Immunology of Shenzhen University, Room A7-509 at Lihu Campus of Shenzhen University. 1066 Xueyuan Blvd, Shenzhen, 518055, China.
Department of General Practice Medicine, Third Affiliated Hospital, Shenzhen University, Shenzhen, China.

Shuo Song (S)

State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen University and Institute of Allergy & Immunology of Shenzhen University, Room A7-509 at Lihu Campus of Shenzhen University. 1066 Xueyuan Blvd, Shenzhen, 518055, China.
Department of General Practice Medicine, Third Affiliated Hospital, Shenzhen University, Shenzhen, China.

Xuejie Xu (X)

State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen University and Institute of Allergy & Immunology of Shenzhen University, Room A7-509 at Lihu Campus of Shenzhen University. 1066 Xueyuan Blvd, Shenzhen, 518055, China.

Shihan Miao (S)

Shenzhen Senior High School Group, Shenzhen, China.

Miao Zhao (M)

Department of Allergy, Longgang ENT Hospital & Shenzhen ENT Institute, Shenzhen, China. miaorzhao@mailnesia.com.

Pingchang Yang (P)

State Key Laboratory of Respiratory Diseases Allergy Division, Shenzhen University and Institute of Allergy & Immunology of Shenzhen University, Room A7-509 at Lihu Campus of Shenzhen University. 1066 Xueyuan Blvd, Shenzhen, 518055, China. pcy2356@163.com.

Classifications MeSH