Exploring the nuclear proteins, viral capsid protein, and early antigen protein using immunoinformatic and molecular modeling approaches to design a vaccine candidate against Epstein Barr virus.

Herpesvirus 4, Human / immunology Humans Capsid Proteins / immunology Epstein-Barr Virus Infections / immunology Epitopes, B-Lymphocyte / immunology Computational Biology / methods Epitopes, T-Lymphocyte / immunology Viral Vaccines / immunology Antigens, Viral / immunology Models, Molecular Molecular Docking Simulation
Bioinformatics EBNA-1 Epstein Barr virus Immunoinformatics Molecular modeling Vaccine

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
22 Jul 2024
Historique:
received: 29 01 2024
accepted: 04 07 2024
medline: 23 7 2024
pubmed: 23 7 2024
entrez: 22 7 2024
Statut: epublish

Résumé

The available Epstein Barr virus vaccine has tirelessly harnessed the gp350 glycoprotein as its target epitope, but the result has not been preventive. Right here, we designed a global multi-epitope vaccine for EBV; with special attention to making sure all strains and preventive antigens are covered. Using a robust computational vaccine design approach, our proposed vaccine is armed with 6-16 mers linear B-cell epitopes, 4-9 mer CTL epitopes, and 8-15 mer HTL epitopes which are verified to induce interleukin 4, 10 & IFN-gamma. We employed deep computational mining coupled with expert intelligence in designing the vaccine, using human Beta defensin-3-which has been reported to induce the same TLRs as EBV-as the adjuvant. The tendency of the vaccine to cause autoimmune disorder is quenched by the assurance that the construct contains no EBNA-1 homolog. The protein vaccine construct exhibited excellent physicochemical attributes such as Aliphatic index 59.55 and GRAVY - 0.710; and a ProsaWeb Z score of - 3.04. Further computational analysis revealed the vaccine docked favorably with EBV indicted TLR 1, 2, 4 & 9 with satisfactory interaction patterns. With global coverage of 85.75% and the stable molecular dynamics result obtained for the best two interactions, we are optimistic that our nontoxic, non-allergenic multi-epitope vaccine will help to ameliorate the EBV-associated diseases-which include various malignancies, tumors, and cancers-preventively.

Identifiants

DOI: 10.1038/s41598-024-66828-x PMID: 39039173
pubmed: 39039173
doi: 10.1038/s41598-024-66828-x
pii: 10.1038/s41598-024-66828-x
doi:

Substances chimiques

Capsid Proteins 0
Epitopes, B-Lymphocyte 0
Epitopes, T-Lymphocyte 0
Viral Vaccines 0
Antigens, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

16798

Informations de copyright

© 2024. The Author(s).

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Auteurs

Elijah Kolawole Oladipo (EK)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.
Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics, Adeleke University, Ede, 232104, Nigeria.

Taiwo Ooreoluwa Ojo (TO)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.
Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria.

Oluwabamise Emmanuel Elegbeleye (OE)

Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria.

Olawale Quadri Bolaji (OQ)

Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria.

Moyosoluwa Precious Oyewole (MP)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.
Department of Biochemistry, Bowen University, Iwo, 232101, Nigeria.

Abdeen Tunde Ogunlana (AT)

Institute of Advanced Medical Research and Training (IAMRAT), College of Medicine, University of Ibadan, Ibadan, 200005, Nigeria.

Emmanuel Obanijesu Olalekan (EO)

Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria.

Bamidele Abiodun (B)

Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria.

Daniel Adewole Adediran (DA)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.

Oluwaseun Adeola Obideyi (OA)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.

Seun Elijah Olufemi (SE)

Division of Vaccine Design and Development, Helix Biogen Institute, Ogbomoso, 210214, Nigeria.

Ahmad Mohammad Salamatullah (AM)

Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, 11, P.O. Box 2460, 11451, Riyadh, Saudi Arabia.

Mohammed Bourhia (M)

Laboratory of Therapeutic and Organic Chemistry, Faculty of Pharmacy, University of Montpellier, Montpellier, 34000, France.

Youssouf Ali Younous (YA)

Evangelical College, BP 1200, N'Djamena, Chad. scientifcresearcher@gmail.com.

Temitope Isaac Adelusi (TI)

Computational Biology and Drug Discovery Laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, (LAUTECH), Ogbomoso, 210214, Nigeria. tiadelusi@lautech.edu.ng.
Department of Surgery, School of Medicine, University of Connecticut Health, Farmington Ave, Farmington, CT, 06030, USA. tiadelusi@lautech.edu.ng.

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Classifications MeSH

questionsmedicales.fr › Virus › Virus oncogènes › Virus tumoraux à ADN › Gammaherpesvirinae › Lymphocryptovirus › Herpèsvirus humain de type 4 › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines de capside › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus oncogènes › Infections à virus Epstein-Barr › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Déterminants antigéniques des lymphocytes B › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Sciences de l'information › Informatique › Biologie informatique › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Déterminants antigéniques des lymphocytes T › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antiviraux › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes viraux › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Exploring the nuclear proteins, viral capsid...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Exploring the nuclear proteins, viral capsid...