Investigation of a fluorescent reporter microenvironment niche labeling strategy in experimental brain metastasis.

Cancer Cell biology Microenvironment

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
19 Jul 2024
Historique:
received: 17 10 2023
revised: 14 12 2023
accepted: 13 06 2024
medline: 23 7 2024
pubmed: 23 7 2024
entrez: 23 7 2024
Statut: epublish

Résumé

Brain metastases are the most common brain tumors in patients and are associated with poor prognosis. Investigating the colonization and outgrowth of brain metastases is challenging given the complexity of the organ, tissue sampling difficulty, and limited experimental models. To address this challenge, we employed a strategy to analyze the metastatic niche in established lesions, based on the release of a cell-penetrating mCherry tag from labeled tumor cells to neighboring niche cells, using different brain metastasis mouse models. We found that CD206+ macrophages were the most abundant cells taking up the mCherry label in established metastases.

Identifiants

DOI: 10.1016/j.isci.2024.110284 PMID: 39040072 PMC: PMC11261144
pubmed: 39040072
doi: 10.1016/j.isci.2024.110284
pii: S2589-0042(24)01509-8
pmc: PMC11261144
doi:

Types de publication

Journal Article

Langues

eng

Pagination

110284

Informations de copyright

© 2024 The Author(s).

Déclaration de conflit d'intérêts

M.M. is currently affiliated to the Institute of Oncology Research (IOR), Bellinzona 6500, Switzerland, and V.W. to TRON-Mainz, Germany. J.A.J. has received an honorarium for speaking at a research symposium organized by Bristol Meyers Squibb and previously served on the scientific advisory board of Pionyr Immunotherapeutics (last 3 years disclosures).

Auteurs

Matteo Massara (M)

Department of Oncology, University of Lausanne, 1011 Lausanne, Switzerland.
Ludwig Institute for Cancer Research, University of Lausanne 1011 Lausanne, Switzerland.
Agora Cancer Research Centre Lausanne, 1011 Lausanne, Switzerland.
L. Lundin and Family Brain Tumor Research Center, Departments of Oncology and Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.

Bastien Dolfi (B)

Department of Oncology, University of Lausanne, 1011 Lausanne, Switzerland.
Ludwig Institute for Cancer Research, University of Lausanne 1011 Lausanne, Switzerland.
Agora Cancer Research Centre Lausanne, 1011 Lausanne, Switzerland.
L. Lundin and Family Brain Tumor Research Center, Departments of Oncology and Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.

Vladimir Wischnewski (V)

Department of Oncology, University of Lausanne, 1011 Lausanne, Switzerland.
Ludwig Institute for Cancer Research, University of Lausanne 1011 Lausanne, Switzerland.
Agora Cancer Research Centre Lausanne, 1011 Lausanne, Switzerland.
L. Lundin and Family Brain Tumor Research Center, Departments of Oncology and Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.

Emma Nolan (E)

Tumour-Host Interaction Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Werner Held (W)

Department of Oncology, University of Lausanne, 1011 Lausanne, Switzerland.

Ilaria Malanchi (I)

Tumour-Host Interaction Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Johanna A Joyce (JA)

Department of Oncology, University of Lausanne, 1011 Lausanne, Switzerland.
Ludwig Institute for Cancer Research, University of Lausanne 1011 Lausanne, Switzerland.
Agora Cancer Research Centre Lausanne, 1011 Lausanne, Switzerland.
L. Lundin and Family Brain Tumor Research Center, Departments of Oncology and Clinical Neurosciences, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.

Classifications MeSH